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SBI3C Mendel’s First Law: The Law of Segregation Two alleles of one gene segregate and recombine independently during the cross. Mendel used 2 purebred organisms: tall and dwarf pea plants. P1: F1: F2: tall x dwarf cross-pollination all tall self-pollination ¾ tall, ¼ dwarf Mendel made a conclusion that tall was dominant, while dwarf was recessive. Letters are used to represent different alleles. A purebred tall plant: TT. A purebred dwarf plant: tt. Homozygous organism: when it has two identical alleles for a given trait. Eg: TT or tt Heterozygous organism: when it has two different alleles for a given trait. Eg: Tt. The tall allele is dominant and is therefore expressed. Dominant allele can be both homozygous (TT) and heterozygous (Tt). ATT: Recessive allele can be only homozygous (tt)!!! Genotype: the genetic make-up of an organism. Phenotype: appearance of the trait in an organism. PUNNETT SQUARE - explains Mendel’s laws - allows predicting the results of a cross. - Was designed for F2 generation, but can be used for other ones. In Punnett Square: - Each off spring will receive one allele from each parent. - Each allele represents a gamete. - Each gamete should have one member of a pair. 1 of 2 SBI3C Using the Punnett Square, you can determine both the genotypes and phenotypes of the offspring. E.g. 1: PxP YY x yy (Yellow x Green) Y y Yy (Yellow) Gametes: F1: F2: Y y Y YY yellow Yy yellow y Yy yellow Yy green This is a monohybrid cross: cross between two heterozygous individuals that differ in one trait. 2 of 2