Download Rabbit anti-14-3-3 Protein Rabbit anti-14-3

Qty: 100 µL
Rabbit anti-14-3-3 Protein
Catalog No. 42-8300
Lot No.
Rabbit anti-14-3-3 Protein
FORM
This affinity-purified rabbit polyclonal antibody is supplied as a 100 µL aliquot in 10 mM HEPES (pH 7.5), 150 mM NaCl
containing BSA and 50% glycerol.
PAD:
ZMD.610
IMMUNOGEN
Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein, conjugated to keyhole limpet
hemocyanin (KLH).
SPECIFICITY
Specific for the ~29 kDa 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption
of the antibody with the C-terminal peptide that was used to generate the antibody.
REACTIVITY
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will
react with bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that
these species have 100% homology with the amino acid sequence used as antigen.
Sample
Rat
Western
Blotting
+++
(Excellent +++, Good ++, Poor +, No reactivity 0, Not applicable N/A, Not determined ND)
Western blot of rat hippocampal (Hipp) lysate showing immunolabeling
of the ~29 kDa 14-3-3 protein.
.
(cont’d)
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected]
PI428300
(Rev 10/08) DCC-08-1089
Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website,
www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
(42-8300 cont’d)
USAGE
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be
affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method,
temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has
not been determined. The following concentration ranges are recommended starting points for this product.
Western Blotting: 1:1000
STORAGE
Store at 2-8°C for up to one month. Store at –20°C for long-term storage. Avoid repeated freezing and thawing.
BACKGROUND
1
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling . The proteins
are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different
neurological disorders2. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues
and regulate the functions of these binding partners by a number of different mechanisms3,4. The 14-3-3 proteins affect a
diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.
REFERENCES
1. Bridges D, Moorhead GB, Sci STKE 2005:re10, 2005.
2. Berg D, et al., Nat Rev Neurosci 4:752-762, 2003.
3. Silhan J, et al. J Biol Chem 279:49113-49119, 2004.
4. Dougherty MK, Morrison DK, J Cell Sci 117:1875-1884, 2004.
RELATED PRODUCTS
Product
Protein A
rec-Protein G
ZyMAX™ Goat anti-rabbit IgG
ZyMAX™ Goat anti-mouse IgG
Secondary antibody conjugates.
Goat anti-rabbit IgG
Conjugate
(H+L)
Alexa Fluor® 488
A11008
Alexa Fluor® 555
A21428
Alexa Fluor® 594
A11012
Alexa Fluor® 647
A21244
HRP
81-6120
AP
81-6122
Biotin
B2770
*Excitation/emission (nm); **Not applicable
Conjugate
Sepharose 4B
Sepharose 4B
Unconjugated
Unconjugated
Cat. No.
10-1041
10-1241
81-6100
81-6500
Goat anti-mouse IgG
(H+L)
A11001
A21422
A11005
A21235
81-6520
81-6522
B2763
Ex/Em*
495/519
555/565
590/617
650/668
NA**
NA
NA
Fluorescence
similar to-FITC
Cy3
Texas Red
Cy5
NA
NA
NA
For additional secondary antibody conjugates, visit www.invitrogen.com/antibodies
For Research Use Only
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected]
PI428300
(Rev 10/08) DCC-08-1089
Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website,
www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.