Download Genome Editing with engineered nucleases poster

GENOME
EDITING
WITH
ENGINEERED NUCLEASES
FOUR FAMILIES
ENGINEERED
MEGA-NUCLEASE
OF DESIGNER RE-ENGINEERED
HOMING
ENDONUCLEASES
ENGINEERED
NUCLEASES
GENOME EDITING
IN WHICH DNA IS
INSERTED, REPLACED, OR REMOVED
USING ARTIFICIALLY
ENGINEERED NUCLEASES,
OR “MOLECULAR SCISSORS”
LEFT TALEN
TRANSCRIPTION
ACTIVATOR-LIKE EFFECTOR
NUCLEASES (TAL EFFECTOR
NUCLEASES)
FOKI
RIGHT TALEN
RIGHT ZFN
FOKI
ZINC FINGER
NUCLEASES (ZFNS)
CAS9 PROTEIN
CRISPR-CAS SYSTEM
(CLUSTERED REGULARLY
INTERSPACED SHORT
PALINDROMIC REPEATS)
GUIDE
RNA
LEFT ZFN
NUCLEASE-MEDIATED
GENOME EDITING
CHROMOSOME &
ENGINEERED NUCLEASE
GENE
DISRUPTION
GENE
INSERTION
GENE
DELETION
INVERSION
SPECIES
DNA-SPECIFIC
DOUBLE-STRANDED BREAK
GENE
GENE
ADDITION CORRECTION
APPLIED IN A DIVERSE
SET OF SPECIES
Human cell lines
& stem cells
Drosophila, insects
C. elegans
Zebrafish
Mice, rats
Pigs
Cows
Plants
Monkeys
HOMOLOGY-DIRECTED REPAIR (HR)
With added homologous DNA:
insert/replace DNA
NON-HOMOLOGOUS END JOINING (NHEJ)
Without added homologous DNA:
repairs with indels
THERE ARE OVER
21,000 PUBLICATIONS
IN THE GENOME EDITING SPACE
# of publications
PUBLICATIONS
≥3,000
≥2,500
≥2,000
≥1,500
≥1,000
≥500
1969
1970
1971
1972
1973
1974
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2007
2008
2009
2010
2011
2012
2013
0
ENTRY OF ZFN
ENTRY OF
TAL EFFECTOR
POTENTIAL AND THE FUTURE
STEM CELL
ENGINEERING
& GENE THERAPY
DISEASE-RESISTANT
TRANSGENIC
PLANTS
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For Research Use Only. Not for use in diagnostic procedures. © 2014 Thermo Fisher Scientific Inc. All rights reserved.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
TISSUE & ANIMAL
DISEASE MODELS
ENTRY OF
CRISPR