Download 27 de febrero de 2012

Recombinant Human Soluble Interleukin 6 receptor
(sIL-6Rα) 6xHis.
Catalogue # /Size:
HIL6RT-05 / 5 μg
IMMUNOSTEP S.L. Registro mercantil de Salamanca, Tomo 259, Libro 0, Folio 206, Sección 8, Hoja SA-7489 – C.I.F. B-37373784
HIL6RT-20 / 20 μg
Source: Full recombinant human protein produced in E. Coli with an aminoterminal hexahistidine tag.
Molecular Weight: 42.25 kDa.
Purity: >95 % as determined by SDS-PAGE analysis.
Amino Acid Sequence: 338 aa
Description: Interleukin 6 receptor (IL6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine
receptor. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an
important role in immune response. Dysregulated production of IL6 and this receptor are implicated in the
pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. In melanocytic
cells IL6R gene expression may be regulated by MITF. The IL-6 receptor system consists of two chains, IL-6R alpha
and gp130. The IL-6R alpha is the binding component specific to the IL-6, but does not transduce a signal alone. On
the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11
and CT-1. The low affinity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble
IL-6R alpha acts as an agonist of IL-6 activity.
Oyher names: IL6R-alpha, Interleukin 6 receptor, Interleukin 6 receptor alpha subunit, B cell stimulatory factor-2,
Membrane glycoprotein 80.
Endotoxin: < 1 EU/µg determined by LAL method.
Formulation: 20mM Tris-HCl, 0.8M Urea & 50% glycerol, pH 8.0.
Storage:. Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
Applications: ELISA, WB, Antibody production.
References:

Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA
microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x.
PMID 19067971.

"Entrez Gene: IL6R interleukin 6 receptor".

Schwantner, Andreas; Dingley Andrew J, Ozbek Suat, Rose-John Stefan, Grötzinger Joachim (Jan. 2004).
"Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR
spectroscopy". J. Biol. Chem. (United States) 279 (1): 571–6. doi:10.1074/jbc.M311019200. ISSN 00219258. PMID 14557255.

Schuster, Björn; Kovaleva Marina, Sun Yi, Regenhard Petra, Matthews Vance, Grötzinger Joachim, RoseJohn Stefan, Kallen Karl-Josef (Mar. 2003). "Signaling of human ciliary neurotrophic factor (CNTF)
revisited. The interleukin-6 receptor can serve as an alpha-receptor for CTNF". J. Biol. Chem. (United
States) 278 (11): 9528–35. doi:10.1074/jbc.M210044200. ISSN 0021-9258. PMID 12643274.

Taga, T; Hibi M, Hirata Y, Yamasaki K, Yasukawa K, Matsuda T, Hirano T, Kishimoto T (Aug. 1989).
"Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130". Cell
(UNITED STATES) 58 (3): 573–81. doi:10.1016/0092-8674(89)90438-8. ISSN 0092-8674. PMID
2788034.
REVISION Nº 2
EMISSION DATE 21-11-2013
HIL6RT

Schooltink, H; Stoyan T, Roeb E, Heinrich P C, Rose-John S (Dec. 1992). "Ciliary neurotrophic factor
induces acute-phase protein expression in hepatocytes". FEBS Lett. (NETHERLANDS) 314 (3): 280–4.
doi:10.1016/0014-5793(92)81489-9. ISSN 0014-5793. PMID 1281789.
*Note: For research use only. Not for use in diagnostic or therapeutic procedures.
REVISION Nº 2
EMISSION DATE 21-11-2013
HIL6RT