Download CORE Ottawa - North American Millers` Association

Collaborative Oat Research
March 7-8, Ottawa
Sponsored by the
North American Millers’ Association
The link between grain and goodness.
What makes oat varieties different ?
How can we use this information
to make better oats?
The CORE advantage
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Engaged stakeholders
Public and collaborative researchers
Broad and deep knowledge diversity
Germplasm diversity
A modern toolbox
The CORE challenge
• Discovery  Transformational research
• Data  publications and solutions
• Recipe book for molecular breeding
• Shared kitchen ?
• Fair information and germplasm exchange
• Toward allele/haplotype sharing ?
• What would you do with your breeding if
– you could automate everything ?
– early generation ? multi-trait index ? ....
Meeting objectives
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Understand what we have done
Understand what we can do with it
Ensure availability
Devise a plan
Share additional foundational research
Share experience with data analysis
CORE
Collaborative Oat
Research Enterprise
Going forward:
Enterprise
or
Engagement ?
First principles (truths/assumptions)
 Traits are genetic (really!)
 Breeders improve based on genetics
 Breeders do better with diversity
 Molecular breeding CAN work
 Will require informatics
Will require teamwork, collaboration, and
TRUST
Warning: there is DNA in your breakfast !
12 billion (12x109) bases
• A single change at one of these bases
could increase fibre....
• But, an adapted oat variety requires
the right DNA at thousands of genes,
millions of bases.
Fun DNA facts
Typing 60 words per minute, eight
hours a day, it would take 50 years
to type ONE COPY of your genome
The DNA in all your cells would
reach the moon 6000 times.
The oat genome is 4 times longer
than the human genome.
Some DNA: genes  proteins
But, most DNA has no known function
?
This is what we were looking for
Allele
A
Functional
Single Nucleotide Polymorphism
= SNP
gene difference
T
SNP
= Marker
= Locus
How we find a useful SNP
Number of varieties
...CGTTGGCGAGA...
120
...CGTTGGCCAGA...
2
...CGTTGGCGAGA...
5
...CGTTGGCCAGA...
300
But we need many SNPs to find this
SNP discovery and GBS
in three easy slides....
Variety 1
Variety 2
Variety 3
SNP
No SNP
SNP
Design SNP assays (Illumina)
.......... Or direct genotype by sequencing (GBS)
Introducing…. 44 great minds
Jim Bair
Aaron Beattie
Åsmund Bjørnstad
Shiaoman Chao
Pete Eckstein
Jason Fradette
Judith Frégeau-Reid
Dennis Galbraith
Steve Harrison
Biniam Hizbai
Catherine Howarth
Gongshe Hu
Yung-Fen Huang
Emir Islamovic
Eric Jackson
Eric Jellen
Dave Kendra
Tim Langdon
Gerard Lazo
Sherri Lehman
Joseph Lutz
Benazir Marquez
Dave Matthews
Michael McMullen
Shea Miller
Steve Molnar
Paul Murphy
Gnanesh Nanjappa
Itamar Nava
Mark Newell
Barbara Podhorodeski
Jesse Poland
Michael Pritchard
Ian Puddephat
Bruce Roskens
Rick Schwein
Nick Tinker
Pernilla Vallenback
Charlene Wight
Weikai Yan
Nancy Ames
Jennifer Mitchell Fetch
Marty Carson
Howard Rines
Housekeeping
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Cell phones, email, wi-fi
Coffee
USB keys, Program
ftp://aowc.ca user: u52591244-core
All eyes on ………
Eric Jackson