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NBE-E4120, Cellular Electrophysiology Exercise 1 Solving instructions 1. An example solution: Using trial and error method, we first select a 10M resistor to limit the currents. Current i divides into two branches, and iin should be ~50 pA. We assume that the current iin will be small compared to other currents -> i β i2 Letβs try selecting a 100 M resistor to the other branch and then calculating the resistance for R. Kirchhoff: 4,5π β π β 10π β ππ = 0 βπ β 10π ππ β πππ π ππ β πππ β 100π = 0 i 10M iin 100M i2 R Rin 2. a) See exercise slides. b) The difference between the recorded voltage and the membrane voltage less than 1%: Combine with the voltage division equation: π ππ > 0,99 π π π = π +π ππ π π β π π β 50 πΊΞ© c) Now we start considering signals that change over time. Therefore, we must apply impedances. Voltage division for the membrane potential and the measured potential (similar than in purely resistive circuit): π= ππΆ π ππ +ππΆ π 1 The impedance of a capacitive component: ππΆ = The impedance of a resistive component: ππ = π π πππΆ The equation that describes the dampening of the signal amplitude as a function of 1 the angular frequency Ο of the signal: π π΄=| |= ππ β(ππΆ)2 1 β(ππΆ)2 +π 2 π The dampening is given in dB: β3 ππ΅ = 20 log10 π΄ By combining two previous equations, the angular frequency that is dampened by 3 dB can be solved -> 99,8 1/s, which corresponds to 16 Hz. Does this have an influence on action potential recording? 3. Noninverting amplifier βππ = π ππ ππ π β ππ = (1 + π π ) ππ ππ ππ β ππ = π π ππ Inverting amplifier ππ = π ππ ππ π β ππ = β π π ππ ππ = π π ππ ππ 4. Familiarize yourself with the extra material of lecture 1 (especially chapter βMeasuring biological signalsβ) and exercise task 2, where you can an example sketches of recording setups. What have you learned (based on task 2 and extra material) about e.g. input resistance of a measurement device or the effect of a micropipette on recording high frequency signals?