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Nucleic Acids Research Volume 16 Number 17 1988 Nudeotide sequence of chicken transforming growth factor-beta 1 (TGF-0 1) Sonia B.Jakowlew*, Pamela J.Dillard, Michael B.Sporn and Anita B.Roberts Laboratory of Chemoprevemjon, National Cancer Institute, Bethesda, MD 20892, USA Submitted July 13, 1988 Accession no. XI2373 Two 1800 base pair long clones representing full-length cDNA copies of chicken TGF-B 1 were obtained and seguenced using methods described previously (1). Chicken TGF-B 1 cDNA codes for a primary gene product of 391 amino acids (Fig. 1) with one additional alanine residue at position 238 of the precursor compared to the 390 amino acid human TGF-B 1 precursor (2). Like mammalian TGF-B 1, the predicted chicken TGF-B 1 precursor protein contains a potential signal peptide (residues 1-21), three potential N-linked glycosylation sites, an arg-gly-asp fibronectin cellular recognition signal (residues 245-247) and a tetrabasic arg-his-arg-arg cleavage site (residues 276-279) . Chicken TGF-B 1 shows 100%, 71%, 78% and 8 0% identity with the amino acid sequences of processed human TGF-Bs 1 and 2 and chicken TGF-Bs 3 and 4, respectively. cccccc<xccccccccccccTACCciGccc«ATTCCGGGCCTCcccTCTCCAGCctTAAArrTGACTcm;^^ 110 CACACTTCCCCACTCTrjGCCTCTCTGCTGAMrfcTg^tcCAAGGACCJTTCTCCGATCCCCGACACGW^ 229 C1CT(XTACCA£MTCTCGCCCATCTCCCTTTTTTCCCTAC»IACCCWUCCCACCCATCJU^U^TCCCTTCCACCTCTCCTCCTCCCTTCTCCCTCACACCCTCAACTTCCCTCCC 348 t r r r r t r ^ T r r T i T T i r f T T T T r i r x j - ^ t r j r r r r T i - i i r i i rrirA^jttm-xttTrrriwirnATTrrir^rrj-j^(^strTr1rrJxir.irj^'rjttttrr:rrrxTirrrrc: 1 _ 25 H t T p Y o | J r o " K m r y V r o " £ T y T « i - L » u T r o * ' l » u r « u ~ l e u l > r o l t u L t u " t r p f e u l t u "vil L « J Thr Pro Cly Arg Pro Ala Ala Gly l a u 467 ATG CCC CCr TCG GGG CCT GGG CTC TIC CCC CTC CTG CTO CCS CTC C1G TGC CTG CTA GTG CIG ACC CCI GGC CGG CCG GCC GCC GGA CTG 50 Sar Thr Cys l y s Thr l l a Asp H«t Glu Lau Val Lys Arg l y s Arg 1 1 * Glu Ala [ l a Arg Gly Gin l l a Lau Sar Lys l e u Arg Lau Ala 557 TCC ACC TGC AAG ACC ATC GAC ATG GAG CTO GTG AAG CGG AAG COC ATC CAG GCC ATT CGC GGC CAG ATT CTt TCC AAG CTT CGG CTC CCC 75 Sar Pro Pro t a r Gin Gly Asp Val Pro Pro C l y Pro Lau Pro Glu Ala Val Lau Ala Lau Tyr Asn Sar Thr Arg Asp Arg Val Ala Gly 6*7 ASC CCC CCC AGC CAG GGG CAC CTG CCS CCC GCC CCS CTC CCT C M GCC GTA CTG GCt CTT TAC AAC ACT ACC CCC GAC CGG CTA CCC GGG Clu t a r Val Clu Pro Glu 737 GAA ACT CTC CM CCG GAG 125 Tyr Asp Lys Ph* Lys G l y 0 2 7 TAT GAT AAA TTC AAG GGC 100 Pro Glu Pro Glu Ala Asp Tyr Tyr Ala Lys Clu Val CCC GAC CCA GAG GCG GAC TAC TAC GCC AAC GAG GTC _ ^ ^ _ Thr Pro His S«r l t u Tyr Hat Lau Ptit Asn Thr Sar ACC CCC CAC ACC TTA TAT ATG CTG 1TC AAC ACG TCG Thr Arg v a l Lau Kat Val Glu f a r Gly Asn Gin l l a ACC CGC GTG CTA ATG GTG GAA ACC GCC AAC CAA ATC 150 Glu L t u Arg C l u Ala Val Pro G l u Pro Val Lau Leu GAG CTC CGG GAA GCC GTC CCC GAA CCT CTA TTG CTC 175 Sar A r t A l a C l u L t u Arg Lau l a u Arg l a u Lys Lau l y s v a l C l u G i n U s v a l C l u l a u Tyr Gin l y s Tyr Sar Asn Asp Sar Trp c l y 9 1 7 ICT CCC CCA CAC CTG CGC CTG CTC ACG CTC AAC TTA AAA GTG GAC CAC CAC GTG GAG CTA TAC CAG AAA TAC AGC AAT CAT TCC TGG GGC 200 Tyr Lau l * t Aon Arg L t u Lffu A l a Pro Sar Asp Sar Pro C l u Trp l t u Sac Pha Asp Vsl Thr G l y v a l v a l Arg Gin T r p L t u Thr Arg 1007 TAC CTC ACC AAC CGG CTC CIG CCC CCC AST GAC TCA CCG GAG 1GG CTG TCC TTT GAT CTC ACC CCA GTT GTG CGG CAC TGG CTG ACC CCC 225 Arg Glu A l a I I * Glu Gly Ptit Arg L t u Str A l a H i s Cys S t r Cys Asp Sar l y s Asp Asn Thr L t u His Val G l u l l a A s n l A l s l G l y Pba 1197 ACA CAG CCT ATA GAC GGT TTT CSC CTC ACT GCC β^ββ 25 CAC TGT TCC TGI GAC AGC AAA GAT AAC ACA CTC CAC CTG GAA ATT AAC|GCA|GGC TTC ° Asn t a r C l y Arg A r f c l y Asp L t u Alo Thr l i e His G l y Hat Asn Arg Pro Pba Lau l t u Lau Hot Ato Thr p r o Lau G l u Arg A l a C l n 12*7 AAT TCI GCC CCC CGG CCT GAC CIG GCC ACC ATT CAC CGC ATG AAC CCG CCC TTC CTG CTC CIC ATG GCC ACC CCG CTC GAG AGG CCC CAC v t a r Ser A r g rtis A r g A r g Ala LSU Asp Thr Asn Tyr C y s Pba Sar S t r Thr Gtu Lys Asn C y s C y s * ' Arg C l n l t u Tyr 275 MS. 1377 CAC CTG CAC ACC TCC CCG CAC CSC CCAGCC £TG GAT ACC AAC TAC TGC TIC AGC TCC ACG GAG AAC AAC TGC TGC GTG CGG CAG CTC TAC 325 1 l a Asp P h t Arg Lys Asp l t u c l y Trp l y s T r p I l a U s G l u Pro l y s C l y Tyr ><s A l a Asn Pfca C y » l t u G l y Pro C y s Pro Tyr I l a ATI CAC TIC CCG AAC GAC CIG GGC TCC AAC TCC ATT CAT GAA CCC A M CCC TAC CAT CCC AAT 1IC ICC CTC CCC CCC TGI CCC TAC AIC 350 Trp S t r L t u Asp Tbr Gin Tyr s t r Lys vsl Lau Ala Lau Tyr Asn C l n U s Asn Pro Gly A l a Sar A l a A l a P r o C v s C y a Val Pro Gin TCG ASC CTA SAC ACI CAG IAC AGC AAG CIC CTC CCT CIC TAC AAC CAG CAC AAC CCC CSC GCG ICC CCC CCS CCC TGC TGC GIG CCS CAS 375 A l a l a u G l u Pro L t u Pro [ I t v a l Tyr Tyr V a l C l y Arg Lys Pro Lys Val G l u Gin L t u Sar Asn Hat l l a Val Arg t a r C y a L y * C y a GCG CIS GAC CCA CTG CCC ATC CIG TAC TAC CTG GGC CCC AAC CCC AAG CIG GAC CAC CTC ICC AAC ATC AIC GIG CCT TCC TCC AAC TGC 191 strlAscha XTCACCCCGCCTGTATTTAAGCACATCCTC! TAAAGGTCCACAGAGG p o l y C " ) » C β’To whom correspondence should be addressed REFERENCES 2,747-755. 8730 1. Jakowlew, S.B. e_fc al. (1988) Mol. Endocrinol. 2. Derynck, R. e£ al. (1985) Nature 316.701-705. © IRL Press Limited, Oxford, England.