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Transcript 
SERUM CONTAINING
MEDIA AND ITS
COMPONENTS
INTRODUCTION
MEANING OF THE TERMS:
1.MEDIA
2.SERUM
MEDIA
Two types:
*Natural Media
*Artificial Media
NATURAL MEDIA
Extracted from biological resources
*CLOTS: Plasma clots
*BIOLOGICAL FLUID: Amniotic fluid.
Pleural fluid.aqueous humour from
eye,serum
*TISSUE EXTRACT: Extract from
lymph,spleen,bone marrow,chick
embryo extract
ARTIFICIAL MEDIA
It is grouped into four classes
*Serum containing media
*Serum free media
*Chemically defined media
*Protein free media
COMPOSITIONS
PROTEINS:
1.FIBRONECTIN: *Attachment
*spreading factors
2.TRANSFERIN: binding protein
GROWTH FACTORS:
1.PGDF: Platelets Derived
Growth Factor
2.EGF: Epithelial Growth factor
3.FGF:Fibroblast Growth Factor
4.IGF: Insulin like Growth Factor
GROWTH HORMONES
Insulin promotes the uptake of
glucose n amino acids
Growth hormones may be present
in serum particularly foetal serum
SERUM MEDIA
ADVANTAGEs
1. complete media
2. Increases the viscosity
3. It acts as a buffher
4. no charged substrate is required
DISADVANTAGES
1.Serum contains growth inhibitor
components
2.targe variation in serum quality from
one batch to other
3.it enhances the chances of
contamination
4.Contains cytotoxic components.
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