* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download HiSeq 2500 Applications Brochure
Eukaryotic transcription wikipedia , lookup
Transcriptional regulation wikipedia , lookup
Silencer (genetics) wikipedia , lookup
Non-coding DNA wikipedia , lookup
Molecular ecology wikipedia , lookup
Epitranscriptome wikipedia , lookup
RNA silencing wikipedia , lookup
Gene expression wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Non-coding RNA wikipedia , lookup
Personalized medicine wikipedia , lookup
Molecular evolution wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Genome evolution wikipedia , lookup
Community fingerprinting wikipedia , lookup
DNA sequencing wikipedia , lookup
Whole genome sequencing wikipedia , lookup
www.illumina.com/hiseq www.illumina.com FOR RESEARCH USE ONLY © 2012–2014 Illumina, Inc. All rights reserved. Illumina, BaseSpace, cBot, CSPro, Genetic Energy, HiSeq, Nextera, TruSeq, the pumpkin orange color, and the Genetic Energy streaming bases design are trademarks or registered trademarks of Illumina, Inc. All other brands and names contained herein are the property of their respective owners. Pub. No. 770-2012-003 Current as of 20 December 2013 HiSeq 2500 applications ® Production power. Power and efficiency for large-scale genomics across a wide array of applications. The advent of next-generation sequencing (NGS) has accelerated genomics at a spectacular pace. The first complete human genome was sequenced in 2003—a feat requiring over 10 years, hundreds of sequencers, and the collaboration of dozens of laboratories around the world. Now NGS has taken us from a genome in a decade—to a genome in a day on the HiSeq 2500 System. Our dedication to making sequencing accessible to everyone has helped drive innovation forward. The HiSeq 2500 System gives you the flexibility to go from multiple genomes in a run, to the fastest whole genome yet. The HiSeq System provides a high-powered, cost-effective solution for large, production-scale projects. Empower your research like never before. Any application. Any study. Any sample size. From sample prep through data analysis, we focus on making applications easy—so you can stay focused on your research. Targeted resequencing, gene expression, whole-genome sequencing, epigenetics, and more—we’ve designed simple, end-to-end solutions for any study. No matter your lab size, research goal, or scale of study. Simplest workflow for all applications. Integrated solutions. Streamlined operation. From sample preparation to data analysis, the HiSeq 2500 is a proven and efficient powerhouse for large-scale genomics. Empowering labs to rapidly and cost-effectively take on a broad range of projects. Easy sample preparation and enrichment Access a family of reagent kits that provide the industry’s fastest and simplest workflows for preparing DNA or RNA samples. Powerful sequencing flexibility Scale the instrument output to fit your immediate sequencing needs. Choose between rapid-run mode for fast results or highoutput mode for more samples. Proven data analysis solutions Go from raw data to meaningful results. Plug into integrated data analysis tools. With our cloud-based computing environment, BaseSpace®, or onsite data processing system, IlluminaCompute™, labs can become fully operational in weeks rather than months—without pre-existing IT infrastructure. Sample Preparation Sequence Analyze The HiSeq System offers a complete, integrated workflow from sample preparation though data analysis. Whole-genome sequencing. Quickly and cost-effectively sequence genomes right in your lab with the HiSeq 2500. Accelerate turnaround time with rapid run mode or, stay in high output mode and sequence multiple genomes in a single run. Both modes produce the industry’s highest data quality, for the most accurate and complete whole-genome sequencing results. Targeted resequencing. From whole exome analysis to custom enrichment panels, choose from a RNA RNA comprehensive suite of targetedSmall resequencing solutions to support16S any study Sequencing Sequencing Metagenomics design. Scalable discovery and cost-effective validation or screening are now at your fingertips. Library QC Whole-transcriptome sequencing. From high-throughput gene expression profiling to deep transcriptional analysis, HiSeq offers unprecedented RNA analysis solutions. Measure abundance of individual transcripts and isoforms, discover new genes, and identify regulatory non-coding RNAs. De novo sequencing and metagenomics. With a unique mix of high output, long reads, and paired-end sequencing, theSmall HiSeq is a powerful tool for de novo sequencing of any size Clone genome. Plasmid Checking Genome Perform true metagenomic analyses by sequencing entire microbial communities to discover important taxonomic and functional information. Regulation RNA-Seq de Sequ ChIP-Seq Tar Reseq Epigenetics and gene regulation. Get a more complete biological story by assessing multiple forms of epigenetic regulation in a single HiSeq run. Study DNA-protein interactions and gene regulation using ChIP-Seq. Quantify DNA methylation down to single base resolution. Sequencing RNA-Seq Amplicon ChIP-Seq Targeted Resequencing ChIP-Seq RNA-Seq de novo Sequencing RNA-Seq Resequencing de novo Sequencing RNA Sequencing Resequencing de novo Sequencing ChIP-Seq RNA Targeted Resequencing Small Genome Resequencing RNA Sequencing Targeted Resequencing Custom Enrichment Small Genome Sm Se RNA Sequencing Custom Small RNA Enrichment Sequencing Small Genome R Clone Checking Small RNA Sequencing Small Genome Custom Enrichment Custom Enrichment Targeted Resequencing 16S Metagenomics Plasmid RNA-Seq Targeted Resequencing Resequencing Small RNA Sequencing Sequencing Custom Enrichment Sequencing Regulation de novo Sequencing Regulation Resequencing Proven Illumina SBS technology. Highest accuracy at any coverage level. Illumina sequencers are the most trusted and widely adopted worldwide for a reason: proven Illumina SBS technology delivers the highest data quality in the industry. Illumina SBS technology supports an unprecedented range of genomic applications, with exceptional data accuracy. It ensures the highest standard of quality for any research project—empowering you to drive your research forward with the maximum confidence. Highest quality data—when answers matter. 16000 HiSeq 2500 generates the highest percentage and yield of data > Q30. Millions of Bases 14000 12000 10000 8000 6000 4000 2000 10 20 30 40 Q Score Quality scores from a human genome, single flow-cell run sequenced in 27 hours, in rapid-run mode, with > 92% of bases above Q30, and 99.9% accuracy. The broadest range of sequencing applications. From whole-genome sequencing to small RNA sequencing, the HiSeq 2500 offers accurate, fast, and cost-effective solutions for any NGS application. Application Recommended Read Length Optimized Kits Whole-Genome Sequencing TruSeq DNA PCR-Free Sample Prep Kit Resequencing 2 × 100–125 TruSeq Nano DNA Sample Prep Kit Nextera DNA Sample Prep Kit TruSeq DNA PCR-Free Sample Prep Kit De Novo 2 × 100–125 TruSeq Nano DNA Sample Prep Kit Nextera Mate Pair Sample Prep Kit Targeted DNA Sequencing Nextera Rapid Capture Exome Kit Exome Enrichment 2 × 75–100 Nextera Rapid Capture Expanded Exome Kit Custom Enrichment 2 × 75 Nextera Rapid Capture Custom Kit RNA-Seq (Expression Profiling) 1 × 50 TruSeq Stranded mRNA Sample Prep Kit RNA-Seq (Whole Transcriptome) 2 × 75 RNA Sequencing TruSeq Stranded mRNA Sample Prep Kit TruSeq Stranded Total RNA Sample Prep Kit Regulation Applications ChIP-Seq 1 × 50 TruSeq ChIP Sample Prep Kit Methylation Analysis 2 × 75 Epicentre EpiGnome Methyl-Seq Kit HiSeq 2500 applications Empower your research with HiSeq. Learn more at www.illumina.com/hiseq