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RNA Polymerases Enzyme Promotor T7 RNA Polymerase T7 Promoter E. coli RNA Polymerase Core Enzyme Poly(A) Polymerase Tailing Kit no Activity Applications T7 RNA Polymerase produces defined Synthesis of RNA for nucleic acid RNA by in vitro transcription of dsDNA amplification methods or gene expression that is downstream of the specific RNA studies polymerase promoter SP6 RNA SP6 Promoter SP6 RNA Polymerase produces defined Synthesis of RNA for nucleic acid Polymerase RNA by in vitro transcription of dsDNA amplification methods or gene expression that is downstream of the specific RNA studies polymerase promoter T7 R&DNA™ T7 Promoter IVT: Enzyme is able to incorporate certain Synthesis of "RNA" transcripts of mixed Polymerase 2´-modified-dNTPs (including but not rNMP/2´-dNMP or rNMP/2´-modifiedlimited to dNTPs having a 2´-fluorine or NMP composition, Synthesis of modified 2´-amino group) "RNA" transcripts that are resistant to RNase A-type RNases Core Enzyme will generate a variety of nonspecific transcripts Studying mechanisms of transcription initiation Poly(A) Polymerase uses ATP as a Poly(A) tailing of RNA for cloning or substrate for template-independent affinity purification, Enhances translation addition of adenosine monophosphate to of RNA transferred into eukaryotic cells the 3´-hydroxyl termini of RNA molecules Artikel Nummern Art. Nr. Bezeichnung Verpackung 140010 E. coli RNA Polymerase Core Enzyme 100 Units 140050 E. coli RNA Polymerase Core Enzyme 500 Units 145102 SP6 RNA Polymerase, 200 U/µl 10000 Units 147101 T7 RNA Polymerase, 200 U/µl 10000 Units 147201 T7 RNA Polymerase, 1000 U/µl 50000 Units 147202 T7 RNA Polymerase, 1000 U/µl 50000 Units 150490 Poly(A) Polymerase Tailing Kit, 4 U/µl 400 Units 150491 A-Plus Poly(A) Polymerase Tailing Kit, 4 U/µl 50 Reactions (400 Units) 150520 T7 R&DNA Polymerase, 50 U/µl 1000 Units 150521 T7 R&DNA Polymerase, 50 U/µl 5000 Units Biozym Scientific GmbH Steinbrinksweg 27 ● 31840 Hessisch Oldendorf ● Tel.: 0 51 52 / 90 20 ● Fax: 0 51 52 / 20 70 ● E-Mail: [email protected] Biozym Biotech Trading GmbH Wehlistraße 27b ● A-1200 Wien ● Tel.: 01 / 334 0156 0 ● Fax: 01 / 334 0156 88 ● E-Mail: [email protected]
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