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Prokaryotic and Eukaryotic Cells Two Main Classes of Cells • Prokaryotic (Bacteria and Archaea) – Pro = “Before”; Karyon = “Kernel” – No nucleus, DNA coiled up inside cell • Eukaryotic (Everything else) – Eu = “True” – DNA inside membrane bound organelle inside cell, the nucleus 3 Domains of Life Eukaryotic Cell (non bacterial) Prokaryotic Cell (bacteria) Cell Size: Prokaryotes and eukaryotic cell Size Differences 1 Prokaryotic Diversity: K. Monera Prokaryotic Cell • 4000 described species – Estimated range 400,0004 million sp. • Minimal structural diversity • Tremendous metabolic diversity • Ecological roles and pathogenesis Bacterial Size, Shape and Arrangement Coccus Bacillus Spiral The Capsule: Frederick Griffith, 1928 Prokaryotic Cell Gram staining: A clinical tool for distinguishing cell wall composition (Gram-positive and gram-negative bacteria) • Enhances virulence: attachment and resistance • Forms the foundation of “biofilms” 2 Common bacterial CELL WALL structures as revealed by Gram staining: Bacterial Motility: Flagella Gram positive: thick peptidoglycan cell wall Gram negative: thin peptido. wall + outer memb. Bacterial Growth: Binary fission a simple division process Ecological Importance Bacteria and Cyanobacteria • Nutrient Cycling: – – – – Decomposers Carbon fixation Nitrogen fixation Others • Symbioses: – Mutualistic: +/+ – Commensalistic: +/o – Parasitic: +/- Movie 1 • Foreign pathogens • Opportunistic pathogens Movie 2 Prokaryotes • Low morphological diversity (how they looks) • HUGE metabolic diversity! All organisms can be divided into either of two major groups based upon their pattern of cell structure: Characteristic Prokaryotic Cell size ave. size 1-10 µm nucleus nucleoid (no membrane) single circ. loop of naked DNA absent chromosomes organelles ribosomes flagella cell wall cell reproduction present as smaller 70S form Solid core made of flagellin present in most sp. as peptidoglycan binary fission Eukaryotic Cell ave. size 10-100 µm membrane bound linear, arranged with histones in pairs present, vary with cell function present as larger 80S form 9+2 arrangement of microtubules absent or different in composition mitosis and sexual reproduction 3 Animal Cell Plant Cell Evolution of Eukaryotic Cells Eukaryotic Cell Structure: The Factory • Limits of the P.M. – Surface area revisited • Differentiation of P.M. • Formation of Organelles – – – – Compartmentalized Reaction centers Increase Membrane Surface area More efficient Metabolism Increase DNA…which means? • Leads to Eukaryotic cells… • Plant and Animal Cell Structure – Nucleus: the control center – Cytoplasm and Ribosomes: site of protein synthesis – Endoplasmic Reticulum: plumbing, lipid and protein synthesis – Golgi Apparatus and the secretory pathway – Lysosomes: digestive activity – Chloroplasts and Mitochondria: food and ATP energy – Vacuoles: storage/digestion – Cytoskeleton: structure and movement – Plant Cell Wall: structure, osmosis and turgor pressure • Family Photos-- structural and functional specializations Animal Cell The Nucleus •Chromatin= DNA + proteins •Nucleolus= site of ribosome production •Endomembrane system •Interrelated membrane “network” inside of cell 4 The Nucleus and Protein Synthesis Ribosomes The “real” Ribosomes • Protein Synthesis • Free- endogenous proteins • Bound- secretory and membrane bound proteins The Endoplasmic Reticulum How the Rough ER Works • Huge Surface Area • General functions • Rough endoplasmic reticulum (RER) – Makes more membranes, – Modifies proteins • Smooth endoplasmic reticulum (SER) – Makes lipids, steroids, hormones, etc – Detox in the liver – Storage 5 The Golgi Apparatus ER and Golgi complex Storage, refinement, and shipping of ER products Works in partnership with ER Lysosomes Figure 4.10 Lysosome formation •Sacks of digestive enzymes which break down molecules and organelles in the cells •Digest food, fight invaders, clean house •pH of 5 •Apoptosis •Disorders Vacuoles Plant Cell • Sacks which can store enzymes, proteins, water, and cellular byproducts, waste. • Plants cells often have large Central Vacuole which holds water and other stuff • Vacuoles also transport things from one organelle to another within the cell 6 Vacuoles Mitochondria and Chloroplasts • Role in Energy Transformation – Photosynthesis – Cellular respiration • Evolution – Endosymbiosis Theory The Chloroplast •Most of the living world depends on chloroplasts for its energy! The Mighty Mitochondria Sites of cellular respiration and ATP synthesis •Two membranes on outside •Complex membrane structure on inside The Cytoskeleton Cilia and Flagella The “skeleton, muscles and highway” of a cell 7 A comparison of the beating of flagella and cilia Support and Joining of Cells Support and Joining of Cells 8