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Anatomy of Skeletal Muscle (Text Pg 36 - 37) Three Layers of Muscle Connective Tissue 1. Epimysium (outermost layer) • Surrounds the entire muscle and separates it from other tissues • Binds the fascicle together • Becomes one with the tendon at either end of the muscle 2. Perimysium (central Layer) • A connective tissue that groups muscle fibres into a Fascicle (fasciculi), and binds fascicles to its neighboring fascicle. • Contains numerous blood vessels and nerves that supply each fascicle. 3. Endomysium (inner Layer) • Surrounds each muscle fiber. • Binds each muscle fiber with its neighbour The Organization Within a Muscle Fibre (Muscle Cell) 1. Sarcolemma • The plasma membrane beneath the endomysium • Contains the muscles cell’s cytoplasm (sarcoplasm) 2. Myofibril • Thread like structures that run the length of the fibre. • ~1-2 μm in diameter • Within these are finer 'thick and "thin" filaments 3. Myofilaments • There are 2 types of myofilaments (Cellular proteins) within the myofibril. – Actin: Thin Filaments – Myosin: Thick Filaments • Each Myosin Filament is surrounded by 6 Actin Filaments • The interaction between actin and myosin allow for muscular contractions to take place. The Sarcomere • A sarcomere is defined as the segment between two neighboring Z-lines • The smallest functional unit of muscular contraction. • Consists of repeating units of Actin & Myosin along the length of the muscle fibre which gives skeletal muscle its stripes. Let’s Draw a Sarcomere!! • M line – Links thick filaments • Z line – Links thin filaments • A band – A-band contains the entire length of a single thick filament (dark band). • H zone – Area of A band that contains only thick filaments. • I band – I-band is the zone of thin filaments that is not superimposed by thick filaments.