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Mendel’s Pea Plants Life Science Unit 7 Lesson 1 Attendance link: http://goo.gl/forms/bOZWikMCIc EXPECTATIONS Required Class Connects Tuesday, Wednesday, & Thursday 1:30-2:30 pm Be active and participate in class. Be respectful to your classmates Be positive in the chat box and use it correctly. Have a working microphone! You will need it during the lessons and break out rooms. If you have a question, please place it in the chat box and repost it if I don’t see it. 2 Objectives • Identify traits as genetically determined characteristics and give examples of traits (for example, eye color, leaf shape). • Distinguish between dominant and recessive traits. • Summarize Mendel's contributions to the field of genetics. • Describe how genetic information is passed from parents to offspring. The study of characteristics transferred from both parents Organisms that produce sexually, two parents Genetics Coded in DNA Examples: Hair color, eye color Things we inherit Where it all started… • Over 100 years ago in a monastery. • A curious Austrian monk named Gregor Mendel started to wonder why pea plants have different colored flowers. Studied the inheritance of traits in pea plants Developed the laws of inheritance Mendel's work was not recognized until the turn of the 20th century (1900’s) Between 1856 and 1863, Mendel cultivated and tested some 28,000 pea plants He found that the plants' offspring retained traits of the parents Called the “Father of Genetics" Mendel stated that physical traits are inherited as “particles” Mendel did not know that the “particles” were actually Chromosomes & DNA Mendel kept detail accounts of his observations and experiments. This helped later scientist confirm his findings. Alleles • Two forms of a gene (dominant & recessive); you get one allele from each parent. Dominant • Stronger of two alleles expressed in the hybrid; represented by a capital letter; only need one copy for it to show. Recessive • Allele that show up less often in a cross, must have two copies for it to show up; represented by a lowercase letter. Genotype Gene combination for a trait • This is the allele make up of a gene/trait. Must show both alleles. • Example: RR, Rr, or rr Phenotype The physical feature resulting from a genotype • This is the trait that is expresses. That is the trait that you see. • Example: Brown hair, blue eyes, two ears, etc. Genotypes Homozygous The gene combination involving 2 dominant or 2 recessive alleles for a gene. Label genes as homozygous recessive or homozygous dominant Heterozygous The gene combination of one dominant and one recessive allele for a gene. Don’t use recessive or dominant Called hybrid Called Pure or true breeders Why peas? Can be grown in a small area Produce lots of offspring Produce pure plants when allowed to self-pollinate several generations Can be artificially crosspollinated Reproduction in Flowering Plants •Pollen contains sperm •Produced by the stamen •Ovary contains eggs •Found inside the flower Pollen carries sperm to the eggs for fertilization Self-fertilization can occur in the same flower Cross-fertilization can occur between flowers copyright cmassengale 12 • Mendel’s Experimental Methods •Mendel hand-pollinated flowers using a paintbrush •He could snip the stamens to prevent self-pollination •Covered each flower with a cloth bag •He traced traits through the several generations How Mendel Began Mendel produced pure strains by allowing the plants to selfpollinate for several generations copyright cmassengale 14 Eight Pea Plant Traits • Seed shape --- Round (R) or Wrinkled (r) • Seed Color ---- Yellow (Y) or Green (y) • Pod Shape --- Smooth (S) or wrinkled (s) • Pod Color --- Green (G) or Yellow (g) • Seed Coat Color ---Gray (G) or White (g) • Flower position---Axial (A) or Terminal (a) • Plant Height --- Tall (T) or Short (t) • Flower color --- Purple (P) or white (p) copyright cmassengale 15 copyright cmassengale 16 copyright cmassengale 17 Mendel’s Experimental Results 18 Generation “Gap” • Parental P1 Generation = the parental generation in a breeding experiment. • F1 generation = the first-generation offspring in a breeding experiment. (1st filial generation) • From breeding individuals from the P1 generation • F2 generation = the second-generation offspring in a breeding experiment. (2nd filial generation) • From breeding individuals from the F1 generation copyright cmassengale 19 Following the Generations Cross 2 Pure Plants TT x tt Results in all Hybrids Tt Cross 2 Hybrids get 3 Tall & 1 Short TT, Tt, tt copyright cmassengale 20 Mendel’s hybridization experiments… Monohybrid crosses: Parental Generation F1 generation True-breeding purple flower x True-breeding white flower All purple flowers (the hybrids) Allowed F1 offspring to self-fertilize F2 generation 705 purple 224 white • Mendel crossed two true breed plants. The first generation of a cross between a true-breeding plant with yellow seeds and a true-breeding plant with green seeds, all the offspring had yellow seeds. The yellow-seed trait was the same trait that only one of the parents had. Where did the green seeds go? The parent with yellow seeds must have been homozygous dominant, YY. The parent with green seed must have been homozygous recessive, yy. Therefore, with each offspring getting one allele from each parent, their genotype must be Yy. Because yellow is dominant it only needs one allele for that phenotype to show up. How can we see if this is true? • Mendel then crossed the offspring of this cross to each other (remember, they all have yellow seeds). In the second generation of plants, Mendel noticed an odd pattern. In each cross, three-fourths of the offspring had yellow seeds, and about one-fourth had green seeds. The green seeds came back! • In the second generation, a cross between two plants grown from first- generation yellow seeds will produce three-fourths yellow seeds and onefourth green seeds. Breakout rooms: Answer this question in complete sentences. • The trait for height in pea plants can be expressed as tall or short. Tall is the dominant expression of the height trait. Short is the recessive expression. A true-breeding tall plant is crossed with a true-breeding short plant, and the first-generation plants are crossed with each other. How will height be expressed in the first- and second-generation plants? Breakout rooms: Answer this question in complete sentences. • The trait for height in pea plants can be expressed as tall or short. Tall is the dominant expression of the height trait. Short is the recessive expression. A true-breeding tall plant is crossed with a true-breeding short plant, and the first-generation plants are crossed with each other. How will height be expressed in the first- and second-generation plants? • In the first generation, all the plants will be tall, but they will all carry one dominant and one recessive allele. In the second generation, three-fourths of the plants will be tall and one-fourth will be short. Breakout rooms (if time) Characteristics You have red hair Soccer is your favorite sport You can roll your tongue You speak Spanish fluently You are colorblind Your hair is dyed brown You have pierced ears You have freckles You prefer sweet food over sour. You can play the saxophone Your blood type is O You have dimples when you smile You are tall for your age Your nails are painted red You enjoy writing poetry Genes/ Inherited Environment Breakout rooms (if time) Characteristics You have red hair Soccer is your favorite sport You can roll your tongue You speak Spanish fluently You are colorblind Your hair is dyed brown You have pierced ears You have freckles You prefer sweet food over sour. You can play the saxophone Your blood type is O You have dimples when you smile You are tall for your age Your nails are painted red You enjoy writing poetry Genes/ Inherited Environment A true-breeding pea plant with greed seeds is crossed with another true-breeding pea plant with green seeds. What kind of seeds will the offspring have? • The offspring will have 100% green seeds. • Green seed color is recessive so the parent generation must both be homozygous recessive and therefore can only pass on the recessive allele. Which statement best summarizes Gregor Mendel’s contribution to science A. Factors for traits in pea plants cannot be controlled. B. Wrinkled seeds grow faster than smooth seeds. C. Garden pea plants can be grown in a variety of colors. D. Factors for some traits are inherited from parents. Assignment • OLS • Part 1 online – 2 questions • Part 2 Offline – 3 questions • Make sure that you are mastering your lessons (80%+). They do not count as part of your progress if they are not mastered. • If you have any questions, please let me know.