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Tear Film Structure and Function Rachel Redfern, OD, PhD, FAAO September 29, 2016 Tear Film • What is the tear film? • What are its characteristics? • What are its components/structure? • Why is it important? • What are some methods for clinical examination? What is the tear film? • Fluid secreted to protect the ocular surface from stress: • Chemical • Physical • Microbial • Serves as the first refractive surface of the eye • Lubrication Normal Tear Film Modified from Pflugfelder, Dry Eye and Ocular Surface Disorders, Elaine Kurie Illustrations Tear Film: Why is it important? • Essential for maintaining: • • • health of the cornea and conjunctiva optical quality of the cornea Disruption leads to: • • compromised corneal and conjunctival physiology reduced visual performance What is the tear film? Normal Tear Film • Exceedingly complex • Composition is dynamic to maintain homeostasis • Components interact to create a hydrated gel Modified from Pflugfelder, Dry Eye and Ocular Surface Disorders, Elaine Kurie Illustrations Tear Film Structure • The anterior lipid layer provides stability by interacting with the mucin-aqueous phase • Secretory mucins mix with the aqueous layer • Membrane-associated mucins on the microplicae of the epithelium form the glycocalyx From Gipson IK, Argueso Tear Film Structure: Lipid Component Meibomian Glands visible through conjunctiva ducts on lid margin Tear Film Structure: Lipid Component Length • Follows the tarsus (4-5mm) Number • Upper lid: 30-40 • Lower lid: 20-30 Volume • Higher in upper lid (26µl vs. 13µl) Relative functional contribution (upper vs. lower) to the tear film lipid layer is unknown… Tear Film: Lipid Component Summary • Outmost, superficial layer of the tear film • Function (s): 1) Reduces evaporation 2) Stabilize the tear film 3) Barrier to pathogens • Complex mixture Tear Film: Aqueous Component • Functions: Protection (e.g., immune defense) Wound healing Stability promoting Enzymes (catalyze other reactions) Metabolic Structural/mechanical Cell signaling Cell adhesion Transport Aqueous! – – – – – – – – – Tear Film Summary • Fluid secreted to protect the ocular surface from stress • Chemical, microbial, physical, hypoxia • Lipid layer = meibomian glands • Aqueous = lacrimal gland • Mucin = goblet cells • Mostly parasympathetic control • Hormonal influences Ocular Surface Lab Tear Osmolarity • • Tear lab Tear ferning Tear Secretion • • Schirmer’s Test Phenol Red Thread Tear Film Stability (Keratograph) • NITBUT Lipid Layer Evaluation • • Meibography (Keratograph) Interferometry (Lipiview) Tear Ferning • Ions in the Tear Film + Sodium and Potassium Chloride and Bicarbonate • Useful in the detection of hyperosmolarity • The shift in the salt-tomacromolecule • • ↓ Proteins & Mucins ↑ Ions TearLab TearLabTM TearLab DEFINITION: number of osmoles (Osm) of solute per litre (L) of solution NaCl dissociates Na+ and Cl− ions 1mole of NaCl becomes two osmoles in solution Tear Production Tear Secretion • • Schirmer Strip Phenol Red Thread Tear Stability • TBUT (SLE optional) • NITBUT Keratograph • Meibography (Keratograph) • IR light at 8001400nm • Increased contrast between the glands and surrounding tissue LipiView Lipid Layer Evaluation • interferometry to measure the lipid layer’s thickness • color assessment of the tear film by specular reflection • interferometric color units (ICU, nm) Questions?