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Name______________________________ Class __________________ Chapter 12 DNA and RNA Date ______________ Chapter Test A Multiple Choice Write the letter that best answers the question or completes the statement on the line provided. ____ 1. What did Griffith observe when he injected into mice a mixture of heat-killed disease-causing bacteria and live harmless bacteria? a. The disease-causing bacteria changed into harmless bacteria. b. The mice developed pneumonia. c. The harmless bacteria died. d. The mice were unaffected. ____ 2. Which of the following is a nucleotide found in DNA? a. ribose + phosphate group + thymine b. ribose + phosphate group + uracil c. deoxyribose + phosphate group + uracil d. deoxyribose + phosphate group + cytosine © Pearson Education, Inc. All rights reserved. ____ 3. DNA replication results in two DNA molecules, a. each with two new strands. b. one with two new strands and the other with two original strands. c. each with one new strand and one original strand. d. each with two original strands. ____ 4. During mitosis, the a. DNA molecules unwind. b. histones and DNA molecules separate. c. DNA molecules become more tightly coiled. d. nucleosomes become less tightly packed. ____ 5. Unlike DNA, RNA contains a. adenine. c. phosphate groups. b. uracil. d. thymine. ____ 6. Which type(s) of RNA is(are) involved in protein synthesis? a. transfer RNA only b. messenger RNA only c. ribosomal RNA and transfer RNA only d. messenger RNA, ribosomal RNA, and transfer RNA ____ 7. During transcription, an RNA molecule is formed a. that is complementary to both strands of DNA. b. that is complementary to neither strand of DNA. c. that is double-stranded. d. inside the nucleus. Chapter 12 Test A 147 Name______________________________ Class __________________ Date ______________ ____ 8. How many codons are needed to specify three amino acids? a. 3 c. 9 b. 6 d. 12 ____ 9. Which of the following terms is LEAST closely related to the others? a. intron c. polypeptide b. tRNA d. anticodon ____10. Which type of RNA functions as a blueprint of the genetic code? a. rRNA c. mRNA b. tRNA d. RNA polymerase ____11. Which of the following is NOT a gene mutation? a. inversion c. deletion b. insertion d. substitution ____12. Which of the following statements is true? a. A promoter determines whether a gene is expressed. b. An expressed gene is turned off. c. Proteins that bind to regulatory sites on DNA determine whether a gene is expressed. d. RNA polymerase regulates gene expression. ____13. A lac repressor turns off the lac genes by binding to a. the promoter. b. tRNA. c. the operator. d. the lac genes. ____15. Which of the following statements is NOT true? a. Mutations do not occur in hox genes. b. Hox genes that are found in different animals are very different from each other. c. Hox genes control the normal development of an animal. d. Hox genes occur in clusters. X A C G A T G C Completion Complete each statement on the line provided. C G 16. The structure labeled X in Figure 12-1 is a(an) ___________________ . T A Figure 12-1 148 Chapter 12 Test A © Pearson Education, Inc. All rights reserved. ____14. Gene regulation in eukaryotes a. usually involves operons. b. is simpler than in prokaryotes. c. allows for cell specialization. d. includes the action of DNA polymerase. Name______________________________ Class __________________ Date ______________ 17. The order of nitrogenous bases in DNA determines the order of ___________________ in proteins. 18. There is no ___________________ that is specified by a stop codon on an mRNA molecule. 19. The lac repressor releases the operator in the presence of ___________________ . 20. In eukaryotes, proteins that attract RNA polymerase bind to ___________________ sequences in DNA. Short Answer In complete sentences, write the answers to the questions on the lines provided. 21. At the beginning of DNA replication, what two processes “unzip” the two strands of a DNA molecule? _________________________________________________________________________________ _________________________________________________________________________________ A B C ine Se rin e Leuc G UC A G UC A A G U C U G U A C top C A S U G teine U Cys G C Valine A C A Stop U G Tryptophan G U e A Arginin C A Leucine C G ne U Seri G U A C A ine C Lys G Pro U lin G e ine CU A ag C A r a U G p G ACU G A C U As nin e C U G A G U A C C U G A A G U C e ionin Meth ine Isoleucine e nin reo e din Th sti e ____________________________ tam ____________________________ Arginin ____________________________ ine os r Ty G Hi ____________________________ C UC A AG Glu © Pearson Education, Inc. All rights reserved. ____________________________ Ala e 23. According to Figure 12-3, what codons specify the amino acid arginine? Glycin ____________________________ cid ____________________________ ic tam cid a ____________________________ ca ____________________________ ____________________________ Glu r ti pa As 22. In Figure 12-2, which molecule is tRNA, and what is its function? Phenylalanine Figure 12-2 Figure 12-3 Chapter 12 Test A 149 Name______________________________ Class __________________ Date ______________ 24. What happens to lac repressors in E. coli when lactose is present? _________________________________________________________________________________ _________________________________________________________________________________ 25. Why are hox genes that are found in different animals very similar to one another? _________________________________________________________________________________ _________________________________________________________________________________ Using Science Skills Use the diagram below to answer the following questions on the lines provided. A A A A A U G U U C Phenylalanine B D Methionine U U U C U A C A A G E A U G U U C A A A G F Figure 12-4 26. Interpreting Graphics What process is illustrated in Figure 12-4? 27. Interpreting Graphics Identify structure C in Figure 12-4. _________________________________________________________________________________ 28. Interpreting Graphics Which labeled structure in Figure 12-4 is a codon? _________________________________________________________________________________ 29. Inferring What is the relationship between the codons and anticodons in Figure 12-4? How is this relationship important? _________________________________________________________________________________ _________________________________________________________________________________ _________________________________________________________________________________ _________________________________________________________________________________ _________________________________________________________________________________ 150 Chapter 12 Test A © Pearson Education, Inc. All rights reserved. _________________________________________________________________________________ Name______________________________ Class __________________ Date ______________ 30. Predicting In Figure 12-4, what will happen after the ribosome joins the methionine and phenylalanine? _________________________________________________________________________________ _________________________________________________________________________________ _________________________________________________________________________________ _________________________________________________________________________________ _________________________________________________________________________________ Essay Write the answer to each question in the space provided. 31. Describe the Hershey-Chase experiment. Why were the results important? © Pearson Education, Inc. All rights reserved. 32. Describe the structure of a DNA molecule. Chapter 12 Test A 151 Name______________________________ Class __________________ Date ______________ 33. Contrast the functions of the three main types of RNA. 34. Mendel might have been surprised to learn that genes simply contain the instructions for assembling proteins. What do proteins have to do with the phenotype of an organism? 35. Why do some kinds of point mutations cause greater changes in proteins than others? © Pearson Education, Inc. All rights reserved. 152 Chapter 12 Test A