Download Nucleotide sequence of the cDNA encoding L

Nucleic Acids Research, Vol. 18, No. 19 5891
© 7990 Oxford University Press
Nucleotide sequence of the cDNA encoding L-histidine
decarboxylase derived from human basophilic leukemia
cell line, KU-812-F
Kohei Yamauchi, Ruriko Sato, Yasuo Tanno, Yuichi Ohkawara, Kazutaka Maeyama1, Takehiko
Watanabe1, Ken Satoh2, Miki Yoshizawa3, Shigeki Shibahara3 and Tamotsu Takishima*
First Department of Internal Medicine, 1 First Department of Pharmacology, institute of Tuberculosis
and Cancer and 3Department of Applied Physiology and Molecular Biology, Tohoku University,
School of Medicine, Sendai 980, Japan
Submitted August 23, 1990
EMBL accession no. X54297
Histamine is a chemical mediator released from basophils or mast
cells and plays an important role in allergic reactions. The
formation of histamine from L-histidine is catalyzed by L-histidine
decarboxylase (HDC, EC4.1.1.22) which is localized in various
tissues including brain, stomach and fetal liver as well as in
basophils and mast cells (1). Recently Joseph et al. isolated HDC
cDNA from rat fetal liver cDNA library and determined its
nucleotide sequence (2). Here we present the nucleotide and
deduced amino acid sequence of the cDNA coding for human
HDC. A cDNA library of human basophilic leukemia cell,
KU-812-F (3), was constructed in the Okayama-Berg expression
vector (4) and screened with the synthetic oligonucleotides
complementary to nucleotide sequence of the rat HDC cDNA
(nucleotide residues 547-596 and 970-1019) (2). One clone
1 AOC CCA COG TTT COC TCT OTT AAA CTC TGC TTC OTO ATT
• far A n Art Fha A n far " • ' Lra Lau Trp Fba Val I U
1200
COO TCC TTC OOO GTG AM AAT CTT CAA OCA CAT OTC ACA CAT OCT ACT CAA ATO OCT AAA
An tar Fba Olr Val Lra Aaa Lau Ola Ala III Val A n ' l a Clr Thr Gla Nat Ala Lra
lltO
M*
ATC CAC TOS CM ATC I
Hat Rla Tr* Ola l l a I
TAT TTT OAA TCT CTO OTCAOAAMOACCCTTCCTTTCAAATTCCTCCCAMAOO CM CTT
Trr Fba Olu t a r Lau Val A n Aao Aap Fro far Fba Glo I l a Fro Ala Lra A n • l l Lau
OOC CTO 0T0 v i i TTT COT CTA AAO OOT CCT AAT TCT CTC ACA CAA AAT CTO TTA A M OAA
oia
Clr L n Val Val Fba A n Lau Lra Olr Fro Aaa Cra Lau Thr Clu Aaa Val Lau in
IMO
ATA CCT AAA OCT OOC COTCTCTTCCTCATCCCCOCCACTATCCMGMAMTTA ATC ATC
l l a Ala Lra Ala o l r A n Lau Fba Lou I l a Fra Ala Thr I l a Ola Aap Lra Lau l l a I l a
14*0
AM
CCT TTC ACT 0T0 ACA TCC CM TTT ACC ACT AOO OAT GM ATC CTC ACA GM TOG AAT CTC
Art Fha Thr Val Thr far Cla Fha Thr Thr A n Aap Aap l l a Lau A n Aap Trp A M L«U
IWO
AM
ATT COA OAT OCT OCC ACTCTCATCCTCAOTCMCMTOTMTTCCCAACCCAOCCCTCOG
IMO
l l a A n Aap . l a Ala Thr Lau I l a Lau far Ola l l a Cra Thr far Cla Fro far Fro A n
lotO
Wt
CM TCT CTC ACT CCC CCA CCA CAT CAT CCA «TC CM OCC MO AM ATC ATC AM CM CCT
Ola t a r Val t a r Clr Ala Clr Aap Aap Fro Val Gla Ala A n Lra l l a I l a Lra Ola Pro
IMO
12*
CM COT OTO OOA OCCaOTCCCATCAAAACGCAAAATaaCCTCCATCTTOAAACCCTOCTC
Ola A n Val Olr Ala Olr Fro Nat Lra Art Cla Aaa Olr •••» " ' • Lau Gla Thr Lau Lau
1740
CAC CCA CTT CAT CAC TCCTTTTCAaAAaMOCCCCAOATOCCACCAMCMAMCTaTCC
Aap Fro Val Aap Aap Cra Fha far Glu Clo Ala Fro Aap Ala Thr Lra l i t Lra lau t o r
CCTGCCTCTCCATCTCTCACAACCACCCMACCCCA ACC CAC ATCi ATC
,
CAC CCT CAC CAC
: Hat Olu Pro Olu Clu
120
a
GOO OOC TCC TCC ACC CTCACAATCTTTTCCAOOTTTCCACAACMATOATOATOCTOAM
Olr Olr tar tar Art Val A n l l a Fba far Arg Fha Fro Ola Aap Nat Nat Nat Lau L»a
TAC ACA CAC ACA COO ACA OAC ATC CTC CAT TAC ATC TCC CAC TAC CTC ACC ACT CTC CCC
Trr A n Clu A n Clr Arg Glo Hat V.I Aap Trr I U Cra Clo Trr Lau Sar Thr Val Arg
ltO
26
AAA ACT CCC TTC AAA AAACTCATCAAATTCTACAOCCrCCCCAOCTTTCCTCAATOCASC
Lra t a r Ala Pbt Lra Lra Lau l l a Lra Fba Trr far Val Fro far Fba Fro Glu Cra t o r
aMAOACCTCTGACCCCACACCTCCMCCTCCCTACCTCCCAGCC CAC CTC CCT CAC ACT
Glu Arf Art Val Thr Fro Ajp Val Ola Fro Clr Tyr Lau Arg Ala Cln Lau Fro Clu t a r
CCTCCTCACCACCCCCACACCTCCCACACCATCTTTCCCCACATT CAA CCA ATC ATC ATC
Ala Fro Clu Aas Fro Aap S«r Trp Aap Sar I I * Fba Glr Ajp I l « Clu Arg H a l l a Hat
MO
M
CCT CCC CTO OTA CAT TCC CAC ACC CCC CAT ATC CAC OCC TAC TAC CCA OCC CTC ACC TCT
rro ClT Val Val Rla Trp Cla far Fro Bla Mac i l a Ala Trr TrrFro Ala Lau Thr f a r
UO
TOCCCCTCCCTCCTAOCAGMATGCTGOCTOATCCCATCAACTGC TTO OCA TTC ACC TOG
Trp Fro S«r Lau Lau Clr Aap Hat Lati Ala AJP Ala l i t Aan Cra Lau Olr Fba Thr Trp
«20
1M
•a
CCATCCAOCCCTCCCTCTACACMCTCCMATGAACGTCATCCAC TOC TTO OCA AAA ATG
Ala S«r S«r Fro Ala Cra Thr Clu Lau Cla Hat Aaa Val Hat Aap Trp Lau Ala Lra Hat
Clr Glr cir Val Lau
M0
1AA
CAOCASACOOTCAGTOAATCCACTTTCATTCCCCTGCTCCCACCA AGO AM AAC AAA ATC
Glo Ola Thr Val far Clu f a r Thr Lau l l a Ala Lau Lao Ala Ala Arg Lra Aaa Lra l l a
MO
114
OTC CTC
CTaaAAATGAAAACOTCTGACCCCaATCCTGATCAOTCCTOCCTA AAT CCC CCA CTC OTC
Lau Glu Nat Lra Thr f a r Olu Fro Aap Ala Aap Clo f a r Cra LauAaa Ala Arg Lau Val
IU
GCCTATGCCTCTCACCAOOCTCACTCCTCTOTCCAAAMGCTGCT TTO ATT TCC CTT CTO
AJa Trr Ala t a r Aap Cla Ala 111 f a r f a r Val Clu Lra Ala C l r Lau l l a f a r Lau Val
720
2M
AACATOAAATTTCTOCCTaTOSATGACAACTTCTCACTCCCACCC CAA CCT CTT C M AAC
L?B Mat Lra Fba Lav Fro Val Aap Aap Aaa Fha f a r Lao Arg Glr Glu Ala Lau C l o Lra
7(0
22a
eCCATCCACCAACACAMCACCCCCCCTTCCTCCCCOTCTTTCTC TCT CCA ACA CTA CCC
Ala l l a Clu Clu Aap Lra Ola Arg Olr Lau Val Fro Val Fha Val Cr* Ala Tar Lau C l r
ACCACTGCSOTCTCTGCATTTGACTCCCnTCACAGCTCOCCCCC ATC TCT CCC CCT C M
Tor Thr C l r Val Cra Ala Fha Aap Cra Lau f a r Glu Lau Glr Fro l l a Cra Ala Arg C l »
NO
2M
CCCCTGTOGCTCCACATCGATCCTCCTTATaCACCCACTCCCTTC CTG TOC CCC OM TTC
Clr La* Trp La* l l a l l a Aap Ala Ala Trr Ala Glr Thr Ala Fha Lau Cra Fro « • Fbt
MO
CCCGOOTTTCTCAACCCCATTCACTATOCCCACTCCTTCACCTTT AAT
Arg C l r Fba Lau Lra Glr l l a C l o Trr Ala Aap Sar Fba Thr Fha Aaa
ATG ATC CTC CAT TTT OAC TOT ACT GOO TTC TOC OTC AM CAC AM TAC
Hat Hat Val I I I Fba Aap Cra Thr Olr Fha Trp Val Lra Aap Lra Trr
CCT
Fro
AM
Lra
TCC AM
f a r Lra
CTC CM
Lau Cla
ACCTTCACTCTCAATCCCATCTACCTCACCCATOCCAACTCACOC OT0 OCC ACC CAC
Thr Fha f a r Val A n Fro l l a Trr Lau A n H a Ala A*a f a r Glr Val Ala Thr Aap
• To whom correspondence should be addressed
IMO
Mt
TOC AM ACT OTO CCA aTOAOTOCTCMAACCCACTCCCCACACMOCCTCTGTOAMWT
Cra Aao t a r Val Fro Val far Ala Ola Lra Fro Lou Fro Thr Gla Ala far Val Lra Ala
ACT GCC CAG CAC TCC CAA CAC CCA ACC CCC CCT CCTCCACGCCTTTCACCCCTTCCACCT
CCC CCA OOC
tit
CTT OCC AM CTC ATC TCCCAAATCAaOOaTOCCAOAOCCTOGCCCnTOCAACOTCC CTT
Val Clr Aaa Lau I l a far Ola I l a A n Olr Ala A n Ala Trp Ala Cra Olr Thr far Lau
TCC TTC CTO TTC MT TMTTCTCTCTCCMACTAMAMAMMOOTGCOCTCCCTCMT
t a r Fba Lau Fha t a r Trr Laa far Val Cln Thr Lra Lra Lra Thr Val A n far Lau t o r
CTCCCACTTCCACACCACTTCTTCCACCACCACCCCACCACCCAC
U u Glr Lau Fro Glu l l a Pha Lau M a l l a l l a Fro S«r f a r Clo
Mi
TCC
Trp
CM
Clo
1020
Ma
TTC
Fba
11M
IMO
tit
TCT CAA TCT OOA CTC CM CTG CCC TOT TCC CCT CTO CM CCC ATC CTT T M ACA C M CCC
t a r Oln Cra Olr Lau Gla Lau Fro Cra Cra Fro Lau Ola Ala Mat Val
1100
Ml
CTT CM CM ACT CTO AOO ATA TAC TTC AOC CAC TCT CTO AAC CCC TCA CAA TTC TAT CCC
lltO
AAC TTT CTC TOC TTA TOT CTA CAT CCA TTT TTC TTO OOO COA OTT CAT AAT TTT AAT CAA
1120
ATT CTC ATA CCC CCT CAT Qtc CCA CAA TAC CAT ACA AAC CAA OM TTT A M CCA OCA TCA
2 2W
TCC AGA TCC CTT CAT C M T C T C C T C M T C A C A A A C C C C C C M C O T A C A C M OCA OCT TCT
IMO
CTC OTT CM CTT CTC ACA TCA TAT ATA ACA CAG AAA TAA ATT ATO CTT CTC CCT OAA AAA
2400
pTN-2, containing an insert of 2.4 kb, is confirmed to encode
human HDC by transient expression of HDC activity in COS
cells (data not shown). Human HDC consists of 662 amino acid
residues (Mr 74,178) and shares 85% homology in amino acid
sequence with rat HDC.
REFERENCES
1. WatanabeJ., Taguchi.Y., Maeyama.K. and Wada.H. (1991) Handbook of
Experimental Pharmacology Vol. 97 (Uvnas.B. ed.), in press, Springer,
Berlin.
2. Joseph.D.R., Sullivan.P.M., Wang,Y.-M., Kozak.C, Fenstermacher.D.A.,
Behrendsen.M.E. and Zahnow.C.A. (1990) Proc. NalL Acad Sri. USA 87,
733-737.
3. Fukuda.T., Kishi.K., Ohnishi.Y. and Shibata,A. (1987) Blood70, 612-619.
4. Okayama.H. and Berg.P. (1982) Mol. Cell. Biol. 2, 161-170.