Download Medical Microbiology, practical protocol

Medical Microbiology, practical protocol
P5 Microbiological diagnosis of STIs 16-20.11.2015
by: Magdalena M-Polanowska 
Name and surname
1. Note your result from urine culture
Number of bacteria / per 1 ml
Types of bacteria
Final result (mark): negative result / contamination / nessesary repetition of urine culture / significant bacteriuria
2. Laboratory diagnosis of gonorrhoea
Tips for success:
(+) single cells in field of view; (++) up to 10 cells in field of view; (+++) above 10 cells in the field of view
A) Direct smear
Components
Qualitative
analysis
Semiquantitative
analysis (+,++,+++,-)
Epithelial cells
Draw the picture from microscope
Lactobacillus
Leucocytes
Yeasts
Other bacteria
Gram (+,-), cocci/rods
B) Culture and identification
Culture media:
Oxidase test (mark true) : negative/ positive result
3.
Laboratory diagnosis of syphilis – observation of FTA - ABS treponemal antibody test
Note the result (mark true): detection of Ag/ detection of Ab
Draw the picture from fluorescent microscope
4. Laboratory diagnosis of Chlamydia trachomatis - observation of DIF test (Ag detection)
Note the result (mark true): detection of Ag/ detection of Ab
Draw the picture from fluorescent microscope
1
5. Laboratory diagnosis of Mycoplasma/Ureaplasma - cultivation in specific liquid medium
Note the result (mark true) : quantitative, semiquantitative/ quantitative analysis
6. Laboratory diagnosis of HPV –laboratory test results
Analyse at least one lab result
Sample:
Method:
Result (HPV oncogenic type/s)
LR
pHR
HR
Interpretation:
7. Crossword about STIs
A) This STI may disseminate when the infected individual has deficiency of late -components of complement
B) Drug of choice (DOC) for treatment of gonorrhoea
C) Oxidase test is a key test for identifying the bacterium (write down a genus name of the bacterium)
D) Symptomatic chlamydial infection acquired by adult, non-pregnant women during sexual intercourse
E) The 1 st most common cause of bacterial STI - write down the species name
F) This provide effective protection against majority of STIs
G) The STI bacterial agent that cannot be cultivated in vitvo – write down species name
H) Which stage of lab processing is a sufficient and reliable diagnosis of urethritis in men with purulent urethral discharge.
I)
This vaccine protects host before genital complications of STI and is available for ….
J)
Which STI results in copious yellowish foul-smelling discharge
K) They are seen as bright green dots under ultraviolet microscope during lab diagnosis of chlamydial STI
L)
Local complication of gonorrhea in women
M) This STI responds to penicillin.
N) Gonococcal infection in males
O) Since it is well known that STIs are not mutually exclusive, which of them is concurrently diagnosed with genital
chlamydial infection
P) Other name of soft chancre caused by Gram-negative bacterium.
Q) What causes vaginitis, that is detected on the basis of wet-mount microscopy (write the genus name)
R) Which specimen is appropriate one for antenatal screening for rubella and syphilis
S) Which sample is recommended for microbiological diagnosis when septic arthritis is suspected
T) This complication is characterized by ocular sticky discharge and is a result of non-venereal transmission.
U) There is systemic disease caused by L1 serotype of Ch. trachomatis
V) Culture-mediated diagnosis is necessary to confirm gonorrhea in..….
W) Drug recommended for pregnant women and babies born to Chlamydia trachomatis- infected mothers
X) The 1st most common cause of viral STI
Y) Which bacterium is investigated in mother’s vaginal and anal swabs before baby delivery- write the species name
Z) Which vaginal disorder cannot be cured with vaginal supplementation of lactobacilli
2
A
4
B
9
C
5
1
D
8
2
E
F
G
3
20
H
I
J
16
K
6
L
18
M
12
N
14
O
P
Q
R
10
S
13
T
15
19
U
V
W
21
X
17
Y
11
Z
7
The particular letters of the password are hidden in the follow ing numerical values from 1 to 21
Write down password:
3