Download Exploring the function of Ypt7-dependent contact sites of the vacuole

S009
Exploring the function of Ypt7-dependent contact sites of
the vacuole membrane
Ayelén González Montoro, Pedro Carpio Malia and
Christian Ungermann
University of Osnabrück, Osnabrück, Germany
Cells require continuous supply of nutrients such as amino
acids, lipids and ions. A central recycling organelle is the yeast
lysosome-like vacuole, which also hosts a major regulator of cell
growth, the TOR kinase complex 1 (TORC1) and its activating
complex, the EGO/LamTOR complex. Apart from continuous
fusion with endosomes and other vesicles, vacuoles form
contact sites with other organelles, whose function is still poorly
understood. We concentrate on the role of yeast Rab7-like
GTPase Ypt7 in signaling processes and contact sites on the
vacuole membrane. We recently identified on a novel vacuolemitochondrial contacts site, called vCLAMP, which is formed by
the HOPS subunit Vps39 and Ypt7. Furthermore, we characterized the IBAR protein Ivy1 as a novel Ypt7 effector on vacuoles
that localizes to vacuolar membrane domains and affects vacuole
biogenesis. Within this presentation, I will provide evidence how
the Rab Ypt7 affects the different contact sites of vacuoles and
how these Ypt7-dependent sites interconnect with other contact
sites of the vacuole.