Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Jason Kenealey, PhD Brigham Young University, ESC S-127 ESC, Provo, UT, 84602. 301-503-2482. [email protected]. Faculty Position Brigham Young University, Assistant Professor, Department of Nutrition, Dietetics and Food Science. (April 2014-present) Research Experience The National Eye Institute, Laboratory of S. Patricia Becerra (August 2011-April 2014) Project Description: Deriving neuroprotective peptides from PEDF Identified neuroprotective PEDF peptides in cell-based assay Identified putative therapeutic PEDF peptides based on binding affinity to the neuroprotective receptor, PEDF-R Used biophysical techniques to characterized the binding interface Established collaborations to model PEDF binding to receptor and high-throughput screening of putative neuroprotective peptides Supervised high school and undergraduate students University of Wisconsin-Madison, Laboratory of Arthur Polans (January 2006July 2011) Project Description: Identification of non-toxic natural products as chemotherapeutic agents Identified apoptotic effect of the natural product-derived calcium signal in cancer cells Demonstrated natural products elicit a unique calcium signal using live-cell imaging Measured uptake of natural products into cancer cells using live-cell imaging Presented data at conferences and in publications Contributed data and ideas for grants Brigham Young University, Laboratory of Gerald Watt (November 2002-June 2005) Project Description: Fe3+ release kinetics of horse spleen ferritin Purified ferritin from horse spleen Demonstrated that ferritin releases Fe3+ from through its 3fold channel. Determined Fe3+ release kinetics Identified the mechanism for Fe3+ release from ferritin Technical Skills: Live-cell calcium imaging (both FRET and small molecule indicators), microscopy, TUNEL staining, tissue culture (primary and cell lines), ELISA, ligand blot, fluorescence polarization, pull-down, protein purification, enzyme kinetics (phospholipase), protein binding, flow cytometry, SDS-PAGE and western blotting, spectrofluorometry, cell fractionation, spectrophotometry Software Skills: Excel, Powerpoint, Graphpad prism, Imagej, Photoshop, Illustrator, FlowJo, Pymol Education B.S. Ph.D. Biochemistry, 2005 Minor in Business Administration Brigham Young University Advisor: Gerald Watt, Ph.D. Biomolecular Chemistry, 2011 University of Wisconsin-Madison Advisor: Arthur S. Polans, Ph.D. Publications Peterson J**, Oblad R**, Mecham J**, Kenealey JD. Resveratrol inhibits plasma membrane Ca2+ ATPase inducing an increase in [Ca2+]I Under Review. *van Ginkel PR, Yan MB, Bhattacharya S, Polans AS, Kenealey JD. Natural products induce a G protein-mediated calcium pathway activating p53 in cancer cells. Toxicol Appl Pharmacol. 2015 288(3):453-62. *Kenealey JD, Subramanian P, Comitato A, Bullock J, Keehan L, Polato F, Hoover D, Marigo V, Becerra SP. Small Retinoprotective Peptides Reveal a Receptor-binding Region on Pigment Epithelium-derived Factor. J Biol Chem. 2015 290(42):25241-53. *Kanavi M, Darjatmoko S, Wang S, Azari A, Farnoodian M, Kenealey JD, van Ginkel P, Albert D, Sheibani N, Polans AS. The Sustained Delivery of Resveratrol or a Defined Grape Powder Inhibits New Blood Vessel Formation in a Mouse Model of Choroidal Neovascularization. 2014 Molecules. 19 (11), 17578-603. Subramanian P, Locatelli-Hoops S, Kenealey JD, DesJardin J, Notari L, Becerra SP. Pigment Epithelium-derived Factor (PEDF) Prevents Retinal Cell Death via Pigment Epithelium-derived Factor-R (PEDF-R): Identification of a Functional Ligand Binding Site. 2013. The Journal of Biological Chemistry. 288 (33): 2392842. Kenealey JD, Colley NJ, Polans AS. Calcium in Vision. Encyclopedia of Metalloproteins. Springer Reference, 2011. Kenealey JD, Subramanian L, Van Ginkel PR, Darjatmoko S, Lindstrom MJ, Ghosh SK, Song Z, Hsung RP, Kwon GS, Eliceiri KW, Albert DM, Polans AS. Resveratrol Metabolites Do Not Elicit Early Pro-apoptotic Mechanisms in Neuroblastoma Cells 2011. J Agric Food Chem. 59(9): 4979-86. Johnson JL, Kenealey JD, Hilton RJ, Brosnahan D, Watt RK, Watt GD. Nonreductive iron release from horse spleen ferritin using desferoxamine chelation. 2011 Journal of Inorganic Biochemistry 105 (2): 202-207. Subramanian L, Youssef S, Bhattacharya S, Kenealey JD, Polans AS, van Ginkel PR. Resveratrol: challenges in translation to the clinic -- a critical discussion. 2010 Clin Cancer Res.16(24): 5942-8. Wilson PE, Nyborg AC, Kenealey JD, Lowery TJ, Crawford K, King CR, Engan AJ, Johnson JL, Watt GD. Evidence for a synergistic salt-protein interaction -complex patterns of activation vs. inhibition of nitrogenase by salt. 2006. Biophys Chem. 122(3):184-94. *Published since coming to BYU **BYU Undergraduates Posters Kenealey JD, Petersen J**, Oblad R**. Resveratrol Inhibits PMCA activity in Breast Cancer Cells. Experimental Biology. Boston, MA, 2015. Kenealey JD, Subramanian P, Hoover D, Becerra SP. Mapping the Receptor Binding Site on PEDF: Implications for a Neurotrophic Role of PEDF-R. ARVO. Seattle, WA, 2013. Kenealey JD, Hoover D, Becerra SP. Mapping the Putative Neurotrophic Receptor Binding Domain on Pigment Epithelium-derived Factor. 15th International Symposium on Retinal Degeneration. Bad Goegging, Germany, 2012. Kenealey JD, Subramanian L, Sareen D, Polans AS. Resveratrol: Discovering Chemotherapy by Fast Chemistry. MathBio: Image Symposium. Madison, WI, 2009. Kenealey JD, van Ginkel PR, Darjatmoko S, Polans AS. Resveratrol Modulates Intracellular Calcium by Opening the 1,4,5 triphosphate Inositol Receptor in Endothelial Cells. 10th meeting of the European Calcium Society. Leuven, Belgium, 2008. Patent Becerra SP, Kenealey JD, Polato FP, Subramanian PP (submitted in 2014) Novel Second-generation PEDF Related Peptides And Methods Of Uses For Treating Ocular Conditions. Tracking number: E-157-2014/0-US-01 (US application). References Dr. S. Patricia Becerra Section Chief National Eye Institute 301-496-6514 [email protected] Dr. Arthur S. Polans M.D. Matthews Professor Departments of Ophthalmology and Visual Sciences UW-Madison 608-265-4423 [email protected]