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Biotech 2C: Manufacturing Technology: Cell Culture, Microbial
Fermentation
Topic
Typical Activity
Overview of Cell Culture
Media preparations
Small Scale Cell Culture
Initiate cultures
Evaluation of Cultures
Morphology/Confluency
Microscopic evaluation
Cell enumeration/Viability
Sterility and Contamination
Quality Control Testing
Subculture by division
Cryopreservation
Freeze cells for storage
Large Scale Media Prep
Preparation and filtration
of media
Mammalian Fermentation
Bioreactor operation
Product Harvest
Cell removal by filtration
Fermentation of Recombinant E. coli
Molecular Overview
Media Preparation
Microbial Media Design
Bacterial growth lab
Microbial Fermentation
OAR, pH, O.D., plasmid retention
Scale-up
Protein Expression/Induction
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