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Cheese Production Using Recombinant Chymosin 1. Gene for chymosin isolated from cow DNA Cow Plasmid DNA 2. Plasmid DNA is cut open with restriction enzymes + 3. Combine chymosin DNA with plasmid DNA Ligase 4. Put recombinant DNA into bacteria Bacteria Bacterial Chromosome Transformation 5. Chymosin protein is expressed in bacterial cells Bacteria Chymosin Chymosin 6. Isolate chymosin protein 7. Isolated chymosin is used to make cheese Cheese © 2010 WGBH Educational Foundation. All Rights Reserved.