Download X-MAN® Cell Lines HAP1 Knockout on Demand

GENOMICS
X-MAN® Cell Lines
HAP1 Knockout on Demand
X-MAN Cell Lines – knockout on demand
10 weeks - $1,980!*
On demand X-MAN Knockout Cell Lines are the most effective and rapid way to
access human endogenous, loss-of-function models for your research. Your cell line
is manufactured by combining the flexibility of CRISPR-Cas9 and the efficiency of
haploid allele modification.
X-MAN®
*Academic pricing
HAP1 Knockout on demand are supplied with a wild type control and a second knockout clone from the off the
shelf collection.
t + 44 (0)1223 655 580 (UK) or +1 (844) 655-7800 (USA)
f + 44 (0)1223 655581
[email protected]
w www.horizondiscovery.com
Horizon Discovery, 7100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom
Key benefits
•
Verified: Frameshift mutation confirmed by Sanger sequencing
•
Fast: Delivered within 8 to 10 weeks
•
Affordable: Knockout cell lines from $1,980* (academic pricing)
•
Proven: Knockout cell lines for more than 1,000 human genes in stock
Brightfield image
Which cells are used for genome editing?
We use a unique comprehensively characterized human cell line known as HAP1 this
cell is particularly suited for genome engineering because it is almost fully haploid.
As a consequence, most human genes are present in a single copy
(see spectral karyotype).
HAP1 – a haploidimage
human cell line
Brightfield
HAP1 can be used to study a great variety of biological processes, such as signal
transduction, cellular trafficking, DNA repair, cell cycle and host-pathogen
interactions.
Spectral karyotype
How are the knockout cell lines generated?
Knockout cell lines are created using
CRISPR-Cas technology leading to
the introduction of small insertions or
deletions that disrupt the reading frame
of the gene.
Clonal HAP1 cell lines are verified by
Sanger sequencing and in many cases,
more than one independent knockout
clone is available.
Exon
1
Exon 2
Exon 3
Cas9-induced double-strand break
Exon
1
Exon 2
Exon 3
Non-homologous end joining
Frameshift mutation
Exon 1
Exon 2
Exon 3
CRISPR-Cas9-mediated genome editing
Selected publications on HAP1
•
Essletzbichler P. et al., Genome Res. 2014 – Genomic characterization of HAP1 cell line.
•
Dong M. et al., Neurology 2014 – HAP1 knockout cell line for evaluation of pathogenic mutations using
phenotype rescue experiments.
•
Kravtsova-Ivantsiv Y. et al., Cell 2015 - HAP1 knockouts of KPC1 and KPC2 support role of KPC1 as E3 ligase that
mediates processing of NF-kB1 p105 to p50.
© X-MAN® Custom Haploid Knockout Service 2015 v-02
t + 44 (0)1223 655 580 (UK) or +1 (844) 655-7800 (USA)
f + 44 (0)1223 655581
[email protected]
w www.horizondiscovery.com
Horizon Discovery, 7100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom
Spectral k