Download UC Davis/NIH NeuroMab Facility

UC Davis/NIH NeuroMab Facility
Department of Neurobiology, Physiology and Behavior, UC Davis, Davis CA 95616-8519
http://neuromab.ucdavis.edu
[email protected]
(530) 752-8398
Anti-Kv4.3 potassium channel subunit, NeuroMab clone K75/41
Immunogen:
Fusion protein amino acids 415-636 (cytoplasmic C- terminus) of rat Kv4.3 (accession number
Q62897)
Human: 99% identity (203/205 amino acids)
Mouse=98% identity (201/205 amino acids)
Monoclonal antibody info:
Mouse strain: Balb/C
Myeloma cell: SP2/0
Mouse Ig Isotype: IgG1
NeuroMab Applications:
Immunoblotting, Immunohistochemistry and Immunoprecipitation
Species Reactivity: human, mouse, rat, Xenopus
No crossreactivity against rat Kv4.2 expressed in transfected cells.
MW: 75 kD in rat brain membrane preparations
Images
Top right. Immunoblot on adult rat brain membranes (RBM), and adult mouse brain membranes
from wild-type (MBM-WT) and Kv4.3 knockout (MBM-Kv4.3-KO) mice. MBM samples courtesy of
Dr. Jeanne Nerbonne, Washington University School of Medicine.
Bottom. Left: Adult rat hippocampus immunohistochemistry. Middle: Electron micrograph shows
K75/41 immunolabelling in the hippocampus using a pre-embedding immunogold method.
Immunogold particles (arrows) were observed along the extrasynaptic plasma membrane of
dendritic shafts (Den) of interneurons establishing synaptic contacts with excitatory axon terminals
(b). Scale bar = 500 nm. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha). Right:
Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and
from human cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].
A cooperative venture among the University of California at Davis, the National Institutes of Health, and Antibodies Inc.