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Section: 3.5 Name: Opening Activity: What is one product of cell respiration? Latin Root Word: Review of Old Information: Sun → Plant ↓ Photosynthesis Step 1__________________________ ↓ Photosynthesis Step 2__________________________ = Glucose ↓ Cell Respiration Step 1__________________________ ↓ Cell Respiration Step 2 if oxygen is not present ________________________ ↓ Cell Respiration Step 2 if oxygen is present ________________________ Step 2 Part 1 ____________________ Step 2 Part 2 ____________________ Reaction of Photosynthesis: Reaction of Cell Respiration: Activity 1: Exercise & Cellular Respiration Lab Purpose: The purpose of this lab activity is to analyze the affect of exercise on cellular respiration. Background: Cells normally contain small amounts of ATP produced during the glycolysis reaction of cellular respiration. Whenever the body needs lots of energy in a hurry, its cells quickly burn this stored ATP (in about 3-5 seconds). For exercise longer than 90 seconds, the cell has to complete two more reactions for energy. These two reactions are called the Krebs Cycle and the Electron Transport Chain. These two reactions are the only way to generate a continuing supply of ATP. This is because the two reactions release energy much faster than Glycolysis and/or lactic acid fermentation. For the first 15 minutes of prolonged activity, your body uses energy stored in glycogen (this is a stored form of glucose). After using the available glycogen, the body begins to burn energy stored as fat. This is one reason why aerobic exercise is so beneficial to your health. Materials: 2 small beakers OR test tubes Phenol Red solution 2 straws stop watch Pre-Lab: Use your background information AND your Cellular Respiration notes to answer the following pre-lab questions. 1. What is the equation for cellular respiration? Label which items are the reactants and the products. 2. What are the 3 reactions of Cellular Respiration? ______________________________________________________________________________ ______________________________________________________________________________ 3. In what part of the cell does aerobic (in the presence of oxygen) respiration occur? __________________________________________________ 4. THINK: After which reaction is CO released? (hint: it is mainly one of the 3 steps) 2 _________________________________ 5. Write a prediction of how exercise will affect your body’s production of carbon dioxide (i.e. do you think your body will produce more or less carbon dioxide as you exercise). Make sure you EXPLAIN WHY you feel that way. ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ Procedure: 1. Label two test tubes A (resting test tube) and B (exercise test tube). 2. Place 10mL of water into each beaker Use the Graduated Cylinder for accurate volume!!! 3. Place 5 drops Phenol Red solution into each test tube. NOTE Carbon Dioxide (CO ) causes Phenol Red to turn Orange. 4. YOUR PARTNER WILL TIME YOU DURING THIS STEP: a. When your partner says “GO” SLOWLY blow air through a straw into the bottom of test tube A. Do not put your straw directly into the solution! CAUTION: DO NOT INHALE THROUGH THE STRAW!!!!!!!!!!!!!!!! b. Record how long it takes for the Phenol Red to change from redorange. Write your results in the table below. 5. When the solution changes color, your partner should say “STOP”. 6. Now run up and down a stair for 1. CAUTION: DO NOT do this if you have a medical condition that interferes with exercise. If you feel dizzy, stop immediately and sit down! 7. Repeat step 4 using test tube B – don’t forget to record your results! 2 Results: Tube A (Resting) Tube B (Exercise) Time (s) Needed to Change Color Analysis & Conclusions: Answer the questions below using your BACKGROUND information in the lab, as well as your lab data. ANSWER THE QUESTIONS IN COMPLETE SENTENCES 1. What was the purpose of this lab? ______________________________________________________________________________ 2. How did exercise affect the time needed for the solution to change color? ______________________________________________________________________________ ______________________________________________________________________________ 3. Why is Aerobic exercise a good thing, (what does it provide your body with)? Activity 2: Across 2. In cellular respiration, series of anaerobic chemical reactions in the cytoplasm that break down glucose into pyruvic acid; forms a net profit of two ATP molecules. 10. Chemical process where mitochondria break down food molecules to produce ATP; the two stages are the krebs cycle, and the electron transport chain. 12. Molecules that absorb specific wavelength of sunlight. 13. In cellular respiration, series of reactions that break down glucose and produce ATP; energizes electron carriers that pass energized electrons on to the electron transport chain. 14. Process by which autotrophs, such as algae and plants, trap energy from sunlight with chlorophyll and us this energy to convert carbon dioxide and water into simple sugars. 15. Series of proteins embedded in a membrane along which energized electrons are transported; as electrons are passed from molecule to molecule, energy is released. Down 1. Anaerobic process where cells convert pyruvic acid into carbon dioxide and ethyl alcohol; carried out by many bacteria and fungi such as yeast. 3. Energy-storing molecule in cells composed of an adensosine molecule, a ribose sugar and three phoshate groups; energy is stored in the molecules's chemical bonds and can be used quickly and easily by cells. 4. Phase of photosynthesis where light energy is converted to chemical energy in the form of ATP; results in the splitting of water and the release of oxygen. 5. Electron carrier molecule; when carrying excited electrons, it becomes NADPH. 6. Reaction taking place in the thylakoid membranes of a chloroplast during the light-dependent reactions where two molecules of water are split to form oxygen, hydrogen ions, and electrons. 7. Light-absorbing pigment in plants and some protists that is required for photosynthesis; absorbs most wavelengths of light except green. 8. Molecule formed from the breaking off of a phosphate group from ATP; results in a large release of energy that is used for biological reactions. 9. Series of reactions during the light-independent phase of photosynthesis in which simple sugars are formed from carbon dioxide using ATP and hydrogen from the light-dependent reactions. 11. Phase of photosynthesis where energy from light-dependent reactions is used to produce glucose and additional ATP molecules. WORD BANK: Photolysis, Electron Transport, Cellular Respiration, Light Reactions, Calvin cycle, Chlorophyll, Alcoholic Fermentation, Photosynthesis, Pigments, Light Independent, Glycolysis, Adenosine Triphosphate, Adenosine Diphosphate, Citric Acid Cycle, NADP