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Transcript
Comparing Prokaryotic and Eukaryotic Cells
Classification of prokaryotic cellular features:
Variant (or NOT common to all)
Cell Wall (multiple barrier support themes)
Endospores (heavy-duty life support strategy)
Bacterial Flagella (appendages for movement)
Gas Vesicles (buoyancy compensation devices)
Capsules/Slime Layer (exterior to cell wall)
Inclusion Bodies (granules for storage)
Pili (conduit for genetic exchange)
Cell walls of Bacteria
1
Cell envelope structure
E. coli structure of
peptidoglycan aka murein
2
Peptidoglycan of a gram-positive bacterium
Bond broken by penicillin
DAP or Diaminopimelic acid
Lysine
3
Overall structure
of peptidoglycan
Cell walls of gram-positive and
gram-negative bacteria
4
Teichoic acids and the
overall structure of the
gram-positive cell wall
5
Summary diagram of the gram-positive cell wall
Cell envelopes of Bacteria
6
Cell envelopes of Bacteria
Structure of the lipopolysaccharide
of gram-negative Bacteria
7
The gram-negative cell wall
N-Acetyltalosaminuronic acid
Pseudopeptidoglycan
of Archaea
8
Paracrystalline S-layer: A protein jacket for Bacteria & Archaea
Formation of the endospore
9
Morphology of the bacterial endospore
(a) Terminal (b) Subterminal (c) Central
10
(a) Structure of Dipicolinic Acid & (b) crosslinked with Ca++
11
Bacterial flagella
(a) Polar (aka monotrichous)
&
(b) Peritrichous
Structure of the
bacterial flagellum
12
Flagellar Motility: Relationship of flagellar rotation
to bacterial movement.
13
Gas Vesicles
(a) Anabaena flos-aquae
(b) Microcystis sp.
14
Hammer & Stopper Experiment
(After)
(Before)
Model of how the two proteins that make up the
gas vesicle, GvpA and GvpC, interact to form a
watertight but gas-permeable structure.
β-sheet
α-helix
15
Bacterial Capsules (a) Acinetobacter sp. (b) Rhizobium trifolii
negative stain
Storage of PHB
16
Sulfur globules inside the purple sulfur
bacterium Isochromatium buderi
Magnetotactic bacteria with
Fe3O4 (magnetite) particles
called magnetosomes
17
EM of Salmonella typhi
“Sex” Pili used in bacterial conjugation of E. coli cells
18