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Test Report Horse’s Name Reliable Man Sire Dalakhani Sex Male Year 2008 SGT Dam On Fair Stage Speed Gene Test Selection and Training A C:T horse has a mixture of speed and stamina and is the most versatile in terms of distance. A C:T horse can perform well as a two-year-old, but is best suited to races between 7 - 12 f (1400 - 2400 m). Only C:T horses are likely to win both one mile (1600 m) and one mile and a half (2400 m) Group races. A C:T stallion is more likely to produce winning two-year-olds if compatible mares are used. The progeny of a C:T mare over her life-span will be C:C (25%), C:T (50%) or T:T (25%), and these proportions can shift towards more speed (C:C and C:T) if a C:C stallion is used or towards more stamina (C:T and T:T) if a T:T stallion is used. Genetic Type (MSTN gene) C:T Best Race Distance Precocity Mid-distance Type (1400 - 2400m) EPT Average age at first win: 36 months C:C Breeding Potential Can produce sprint (C:C), mid-distance (C:T) or staying (T:T) type horses C:T 50% C:C 50% C:T C:T T:T 25% C:C 50% C:T 25% T:T 50% C:T 50% T:T Elite Performance Test Potential for elite performance (Main Panel) Potential for elite performance (Secondary Panels) Mid-distance Panel is most critical for C:T horses. C:T Class 2 (Middistance) is 1.64 times more likely than average to win a Black-Type race in its lifetime. CT Class 2 horse with SP panel score 2 has a 18% higher expected total earnings than CT Class 2 horse with SP panel score 4 Influence on optimum race distance Note: Sprint and Stamina panels have no observed effect on distance for C:T horses. Genetic Type (MSTN gene) C:T Sprint Panel 2 C:T Class 2 horse with Stamina panel score 2 has a 16% higher expected total earnings than C:T Class 2 horse with Stamina panel score 4. Mid-Distance Panel 2 Stamina Panel 2 Tests Explained SGT Speed Gene Test Best race distance and precocity potential in Thoroughbred racehorses can be predicted by the DNA analysis of the MSTN gene, a major regulator of muscle mass development and muscle fibre type. Genetic Type In the MSTN gene, a position in the DNA code contains either the DNA variant C or the DNA variant T. Each individual has two copies of the gene—one inherited from the dam, one from the sire, so there are three possible genetic combinations: C:C, C:T or T:T. Breeding Implications Precocity Optimum Race Distance C:C Fast, Speedy Sprint Type (1000 - 1600m) Average age at first win: 32 months Can only produce sprint (C:C) or mid-distance(C:T) type horses C:T Mid-distance Type (1400 - 2400m) Average age at first win: 36 months Can produce sprint (C:C), mid-distance (C:T) or staying (T:T) type horses T:T Enhanced Stamina (2000m and over) Average age at first win: 40 months Can only produce mid-distance (C:T) or staying (T:T) type horses EPT Elite Performance Test Equinome has developed large panels of DNA variants that can now be used to distinguish between elite performers and poor performers within each distance category, which is first determined for a particular horse using the Equinome Speed Gene Test. Approximately 80 genetic variants are tested for each panel. The combination of advantageous DNA variants determines whether a horse is genetically Class 1, 2, 3, or 4 on each panel, with Class 1 having the highest potential and Class 4 the lowest potential for elite race performance (Black Type race win. 3 Genetic potential for elite performance vs. typical 2 Typical potential for elite performance 1 0 1 2 3 4 Class Score Sprint Panel this panel includes analysis of genes with functions associated with: • • • • • gluconeogenesis central nervous system development insulin regulation lactate metabolism vascularisation Mid-distance Panel this panel includes analysis of genes with functions associated with: • • • • muscle contraction oxidative phosphorylation heart function growth promotion Stamina Panel this panel includes analysis of genes with functions associated with: • • • • muscle contraction oxidative phosphorylation heart function growth promotion The Elite Performance Test Mid-distance panel has a minor additional influence on best race distance. Tests Explained PHT Projected Height Test Mature height at withers can be predicted within 2.54cm (1 inch) with a 70% success rate from birth, based on DNA analysis of the LCORL/NCAPG gene region and the sex of the horse. Genetic Type Physical Type At the LCORL/NCAPG gene region, a position in the DNA code contains either the DNA variant G or the DNA variant A. Each individual has two copies of the gene— one inherited from the dam, one from the sire, so there are three possible genetic combinations: G:G, G:A or A:A. Breeding Implications Mature Height At Withers +/- 1 inch C:C Small 161.08cm (approx. 15.3hh) (female) 162.8cm (approx. 16.0hh) (male) Can only produce small or medium-height horses C:T Medium 163.44cm (approx. 16.0hh) (female) 165.14cm (approx. 16.1hh) (male) Can produce small, medium or tall-height horses T:T Tall 168.28cm (approx. 16.2hh) (female) 168.28cm (approx. 16.2hh) (male) Can only produce medium or tall-height horses Breeding SGT PHT Speed Gene Test The potential outcomes from any breeding combination can be predicted if the genetic type of the mare and stallion are known, as shown in this table. This test can be used with current selection tools to shortlist mares and stallions most likely to produce the desired result. Projected Height Test This information can be used by breeders to produce horses of a desired height more consistently. If the stallion and mare genetic types are known, the potential outcomes can be predicted as per the table below. A:A A:G G:G A:A 100% A:A 50% A:A 50% A:G 100% A:G 50% C:T 50% T:T A:G 50% A:A 50% A:G 25% A:A 50% A:G 25% G:G 50% A:G 50% G:G 100% T:T G:G 100% A:G 50% A:A 50% A:G 100% G:G C:C C:T T:T C:C 100% C:C 50% C:C 50% C:T 100% CT C:T 50% C:C 50% C:T 25% C:C 50% C:T 25% T:T T:T 100% C:T 50% C:C 50% C:T