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DNA & Protein synthesis Assignment 1. a. Draw and Label a double helix of DNA with adenine, cytosine, guanine, and thymine, deoxyribose sugar and phosphate. Explain complimentary base pairing b. Draw the steps of DNA replication. Below the illustration, describe the importance of each of the following terms. i. DNA Ligase ii. Helicase iii. DNA polymerase iv. RNA Primase v. Okazaki fragments 2 2. Explain what semi-conservative means in DNA replication C. Genetic Materials and Protein Production 4. Where will transcription occur in the cell? Draw and Label the process of transcription including the following terms: a. RNA polymerase b. complementary c. Promoter sequence d. Terminating sequence 3 4. Once the mRNA has been constructed during transcription, describe what happens to it. Ie) How is it processed? 4 5. Draw and label the process of translation using following terms. Then, describe the steps involved in protein synthesis. The following terms must be highlighted or underlined in your paragraph. a. mRNA b. Ribosome c. Codon d. Start codon (Met) e. tRNA/anticodon/amino acid f. initiation g. elongation h. termination i. release factor j. polypeptide chain 5 D. Mutations 6. Explain the meaning of the following mutations: frameshift nonsense missense neutral silent 7. Give examples of genetic disorders resulting from DNA mutations. For one of these indicate how protein synthesis is affected. E. Recombinant DNA 8. a. Define. b. Briefly describe the process that produces r-DNA c. How is r-DNA used in current research and medical science. Ie) Give examples of r-DNA