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Mitosis Analysis Onion Root Tip Name _____________________________________________ Period _________ Date ________________________ In this activity, you will be able to: • Identify cells in the various phases of its life cycle, including the phases of mitosis. • Determine the percentage of time cells spend in each phase of its life cycle. Part 1: Identify the phases of mitosis in plant cells 1. Look at the arrows in each box below. 2. Write the correct name of the phase of mitosis in the box next to each picture. 3. Explain how you can tell it is that phase in the last box (hint: telophase and cytokinesis are combined into one phase). Picture © 2010 K.Corbett Phase How can you tell? Part 2: Calculate the percentage of time spent in each phase. 1. Use the image above to count the number of cells in each phase of its life cycle. Record your answer in the table below. 2. Count the number of cells in each phase. Record your answers in the table below. Hint: count the cells for prophase, metaphase, anaphase, and telophase/cytokinesis. Whatever is left over from the total is the number of cells in interphase. 3. Calculate the percentage of time these cells spend in each phase by dividing the number of cells in that phase by the total number of cells and report your answer as a percentage in the table below. Phase Total number of cells Prophase Metaphase Anaphase Telophase/cytokinesis Interphase © 2010 K.Corbett Number of cells Percentage of cells ----- n/a ----- Part 3: Pie chart of cell life cycle 1. Make a pie chart showing the percentage of time the onion root tip cell spends in each phase of its life cycle. Put all of the mitosis phases in order next to each other in the pie chart. Part 4: Observe the onion root tip under the microscope. 1. Look at an onion root tip under the microscope. Find a cell that is undergoing a step of mitosis. Draw it using high power on the microscope in one of the circles below. Find another cell undergoing a different phase of mitosis. Draw it in the second cell using high power. Find a third cell undergoing a different phase of mitosis and draw it in the third circle using high power. Label the phase in each drawing. © 2010 K.Corbett Part 5: Conclusions 1. Which phase has the highest percentage of time spent in that phase? Why? _________________________________________________________________________ _________________________________________________________________________ 2. How does the picture on page 2 of this lab show that mitosis is a continuous process and not a series of separate events? _________________________________________________________________________ _________________________________________________________________________ 3. The onion plant began as a single cell. It had “X” number of chromosomes (the exact number isn’t important, we’ll just call it “X”). How many chromosomes are in each of the cells you observed (give your answer in terms of “X”)? How do you know? _________________________________________________________________________ _________________________________________________________________________ 4. Summarize what you learned in this lab. _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ © 2010 K.Corbett