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THE USE OF SPECIAL HISTOLOGICAL STAINS AS KEY MARKERS IN NEUROTOXICITY Authors: Alina DAN, Cecilia DANCIU Coordinator: TĂBĂRAN Flaviu University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372, Cluj-Napoca, Romania: [email protected] ABSTRACT Due to the structural and functional complexity of the central nervous system, toxicological studies involving this system presents many technical features of achievement, conditioning the results and the relevance of the study. The purpose of this study was to implement the protocol for harvesting, processing and histological staining (Classic staining: HE, special : Luxol Fast Blue, Bielschowsky, cresyl violet and Fluoro-Jade-B) for central nervous system recommended by the Society of Toxicological Pathology (STP) and the European Society of Toxicological Pathology (ESTP) as a best practice guide in neurotoxicity assessment. The use of recommended trimming techniques, manage overall to expose the nervous submain, which has to be examined in terms of toxicity and implementing special staining techniques, whom provides a good differentiation between the nervous lesions and structural artifacts (eg "dark neurons"). Implementation of technical guidelines recommended by STP / ESTP increase the relevance and reliability on histopathological exam from neurotoxicity studies. KEY WORDS Neurotoxicity, best practice guides, special histological techniques REFERENCES Bolon, B., Garman, R., Jensen, K., Krinke, G., & Stuart, B. (2006). A ‘best practices’ approach to neuropathologic assessment in developmental neurotoxicity testing—for today. Toxicologic pathology, 34(3), 296-313. ***http://www.goreni.org/ ultima accesare 24.03.2014.
