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Cell Architecture Please activate your clickers Classification of organisms “Five kingdom system:” Divide all organisms into groups (kingdoms)-different groups have different combinations of cell parts (organelles) ! Prokaryota ! Animalia ! Plantae ! Fungi ! Protista Classification by molecular biology Modern systems divide organisms into groups-(domains, kingdoms)—on the basis of DNA sequences The more we learn, the more complex the situation This chart only classifies eukarya, again on the basis of DNA us S.L. Baudauf, Science 300:1703, 13 June 2003 Prokaryotes ! Bacteria, Archaea ! Simplest structure Plasma Membrane Thin sheet 7-9 nm (0.007-0.009 !m) thick Composition: lipid (fat) and protein Found in all cells Functions: ! serves as platform for biochemical reactions ! recognizes outside stimuli ! controls transport of molecules (and larger things) into and out of cell. Cell Wall Thick net (variable width) surrounding the plasma membrane; rigid Found in most prokaryotes (bacteria, archaea); also plants, fungi, some protists Composition: peptidoglycan, cellulose, chitin, etc. (long molecules, porous net) Functions ! limits the size of cell ! controls cell growth Nucleoid Nucleic acid (DNA, a long, stringy molecule) in center of cell Function: contains information that controls... ! cell functions (metabolism) ! growth of cell ! reproduction Ribosomes Small, solid spheroids (0.02 !m diameter) Nucleic acid (RNA) and protein, two subunits each Ave. 30,000 per cell Function ! synthesis of proteins Eukaryotes ! Plants, Animals, Fungi, Protists ! Complex cell structures ! Multicellular organisms, with specialized cells Organelles in prokaryotes that are the "same" in eukaryotes Plasma membrane Cell wall (in plants, fungi, some protists) Ribosomes Nucleus (nuclei) Spheroid, 10-20 !m diameter Present in all eukaryotes: usually one per cell (more? coenocytic or syncitial) Surrounded by nuclear envelope, a double membrane Chromatin (DNA + protein): ! Nucleosomes ! Chromosomes ! Centromeres ! Telomeres Nucleolus: makes ribosomes Function: like nucleoid of prokaryotes Mitochondrion (-dria) Spheroid ca. 1 !m diameter Surrounded by a double membrane Crista (-ae): inner membrane folds Matrix: liquid gel center Function: directs energy transformation, metabolism Found in all eukaryotic cells: 1 to 10,000 (ave. 100) per cell Mitochondria in cornial cells are long and tubular a and d and green in c and f is autofluorescence b and e and red in c and f is a fluorescent dye absorbed by mitochondria Plastid Spheroid 5 !m diameter Found in plants and some protists ! Double membrane ! Thylakoids ! Stroma Functions depend on type: ! Chloroplast (green) ! Leukoplast (white) ! Chromoplast (colored) ! Proplastids Organelles in prokaryotes and eukaryotes ! Plasma membrane ! Cell wall (in prokaryotes, plants, fungi, some protists—not animals) ! Ribosomes ! Nucleoid/nucleus ! Mitochondrion (eukaryotes, not prokaryotes) ! Plastids (plants, protists, not prokaryotes, fungi, or animals)