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Transcript 
POLYMERASE CHAIN REACTION
AMPLIFYING DNA
• What do you need to replicate DNA?
http://www.youtube.com/watch?v=uD
umZT5z5R8
http://www.dnalc.org/view/15475-The-cycles-of-thepolymerase-chain-reaction-PCR-3D-animation-with-noaudio.html
WHICH DNA SEQUENCE IS AMPLIFIED?
TANDEM REPEATS (VNTR)
TANDEM REPEAT GENES
5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’
5’TTAACCTTAACCTTAACC 3’
DETERMINING YOUR ALLELES
CREATING DNA FINGERPRINT USING
GEL ELECTROPHORESIS
SAMPLE GEL
5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’
5’TTAACCTTAACCTTAACC 3’
Ladder
Suspect 1
Suspect 2
Crime Scene
HOW UNIQUE ARE YOU?
RFLP
RESTRICTION FRAGMENT LENGTH POLYMORPHISM
EXAMPLE
USE TTCC RESTRICTION ENZYME
DNA SAMPLE ONE:
ACTGTTCCACACTTCCGCACATCTTAATTCC
DNA SAMLE TWO:
GTTCCATGCCGAGTACGTTCCATGCTA
I AM YOUR FATHER….WELL MAYBE
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