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Transcript 
DNA Backbone
5’
1’
4’
2’
4’
1’
3’ 2’
DNA Backbone
Describe the chemical structure of the DNA
backbone.
What is meant by 5’ to 3’?
Antiparallel.
Nitrogenous Bases
Complementary Base Pairing
Chargaff’s Rule
 ?
Base pair diameter is 2 nm.
Overall DNA Structure
Right handed helix
0.34 nm between
adjacent base pairs
One turn of the helix is
10 base pairs (3.4
nm)
Genetic Mechanisms
Replication
Transcription
Translation
pp 282 - 290
Practice Questions pg 290, #3, 7, 8, 10
DNA Replication
Replication Theories
1958 – Meselson & Stahl
Grew E. coli in 15N
media
Start the experiment
with DNA that only
contained 15N atoms.
Replication Theories
Only one DNA is
labeled
15N-labeled
DNA
One strand of
both DNAs
labeled
Both strands of
both DNAs
labeled
Meselson & Stahl Experiment Conclusion
What was the conclusion of their experiment?
.
Replication
Three main steps to replication:
1.
2.
3.
1. Replication Initiation
Origins of replication (ori)
1. Replication Initiation
DNA helicase –
single-stranded binding proteins (SSBPs) –
Know the opposite concepts of



Denaturing
Reannealing
DNA Replication Animation
Helicase Animation
DNA Helicase Activity
1. Replication Initiation
replication fork –
1. Replication Initiation
DNA gyrase –
1. Replication Initiation
replication bubble –
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