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Toxicological interactions between the mycotoxins deoxynivalenol, nivalenol and their acetyl
derivatives in intestinal epithelial cells
Archives of Toxicology
Imourana Alassane-Kpembi, Olivier Puel and Isabelle P. Oswald
Corresponding author: Isabelle P. OSWALD, INRA, UMR 1331 Toxalim, Research Center in
Food Toxicology, 180 chemin de Tournefeuille, BP93173,31027 Toulouse cedex 03,FRANCE.
[email protected], Tel: 33 (0) 5 61 28 54 80
Online Resource 1
Online Resource 1 : Chemical structures of DON and NIV and their acetyl derivatives
140
Viability
CellCell
Viability
(%)(%)
80
80
60
60
40
40
20
20
Online Resource 2
0
0 0.1
0.1
10
10
100
100
Conc NIV vs moy NIV
Dose
MoyNIV
DON
Conc DON
NIV vsvsmoy
Conc
vsvs
Moy
FXDON
Dose FX
DON
Moy
Conc 15AD
Moy
FX vs vs
Moy
FX15AD
Conc 15AD vs Moy 15AD
15-ADON
15-ADON
DON
DON
NIV
NIV
FX
FX 3AD vs Moy 3AD
Conc
Conc 3AD vs Moy 3AD
3-ADON
3-ADON
120
100
Cell Viability (%)
1
1
Mycotoxin concentration (
Mycotoxin concentration (
80
60
40
20
0
0.1
1
10
100
Mycotoxin concentration (
Conc NIV vs moy NIV
Dose DON vs Moy DON
Conc FX vs Moy FX
Conc 15AD vs Moy 15AD
15-ADON
DON
NIV
FX
Conc 3AD vs Moy 3AD
3-ADON
Online Resource 2 : Comparative toxicity of individual mycotoxins on proliferating IPEC-1
cells. Intestinal epithelial cells were exposed for 24 hours to serial dilutions of toxins.
Cytotoxicity was assessed by the MTT assay. Data are means ± SD of three independent
experiments.
Online Resource 3
5
DON (µM)
50% cytotoxicity
30% cytotoxicity
10% cytotoxicity
2.5
0
0
1.5
3
15-ADON (µM)
Online Resource 3: Isobologram illustrating the combined cytotoxicity of DON and 15ADON at concentrations eliciting 10% ( ), 30% ( ) or 50% ( ) toxicity. The points are mean
concentrations of dose-response MTT test cytotoxicity curves for each toxin or toxin
combination (Compusyn software analysis).