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Microscopes and Microscopy Quiz Name:_______________ Class:_________ Date:_______________ 1. Base your answer to the following question on the diagram of a microscope below and on your knowledge of biology. 6. Base your answer to the following question on The diagram below shows how a coverslip should be lowered onto some single-celled organisms during the preparation of a wet mount. Why is this a preferred procedure? 1) 2) 3) 4) The coverslip will prevent the slide from breaking. The organisms will be more evenly distributed. The possibility of breaking the coverslip is reduced. The possibility of trapping air bubbles is reduced. 7. Base your answer to the following question on A compound light microscope is represented in the diagram below. State two ways the image seen through the microscope differs from the actual specimen being observed. 2. A student prepared a slide of pollen grains from a flower. First the pollen was viewed through the low-power objective lens and then, without moving the slide, viewed through the highpower objective lens of a compound light microscope. Which statement best describes the relative number and appearance of the pollen grains observed using these two objectives? 1) low power: 25 small pollen grains; high power: 100 large pollen grains 2) low power: 100 small pollen grains; high power: 25 large pollen grains 3) low power: 25 large pollen grains; high power: 100 small pollen grains 4) low power: 100 large pollen grains; high power: 25 small pollen grains 3. While viewing a specimen under high power of a compound light microscope, a student noticed that the specimen was out of focus. Which part of the microscope should the student turn to obtain a clearer image under high power? 1) eyepiece 3) fine adjustment 2) coarse adjustment 4) nosepiece 4. After switching from the high-power to the low-power objective lens of a compound light microscope, the area of the low-power field will appear 1) larger and brighter 3) larger and darker Which microscope part is correctly paired with its function? 1) A – magnifies the image of the specimen 2) B – used for focusing only when the high-power objective is used 3) C – provides the field of view with the largest diameter 4) D – holds the specimen on the stage 8. The total magnification of an image formed by a compound light microscope is a result of the combined magnifications of the 2) smaller and brighter 4) smaller and darker 5. What is the highest magnification that can be obtained with a microscope that has a 10× eyepiece with 10× and 43× objectives? 1) 43× 2) 100× 3) 430× 4) 4300× Page 1 1) 2) 3) 4) eyepiece and diaphragm objective and mirror eyepiece and objective low-power objective and high-power objective 9. Base your answer to the following question on Which sequence contains the correct order of steps for a student to follow to observe the nucleus of protozoa in a stained wet mount, using a compound light microscope? 1) 1 2) 2 3) 3 4) 4 10. Base your answer to the following question on the diagrams below and on your knowledge of biology. 13. Which paragraph describes the correct procedure for preparing a stained wet mount of onion epidermis? 1) Place a piece of onion epidermis on a slide. Add two drops of water and one drop of stain. Cover the slice by dropping a cover slip directly on top of it. Press the coverslip to force air bubbles out. Add one drop of water to one edge of the coverslip, and add one drop of stain to the opposite edge. 2) Add one drop of stain to a piece of onion epidermis. Using forceps, place the epidermis on a slide. Blot the epidermis with a piece of paper towel to remove the excess stain. Drop a coverslip onto the specimen. 3) Place a piece of onion epidermis on a slide. Add one drop One similarity between all the microscopes shown is that they 1) 2) 3) 4) lack a diaphragm and coarse adjustment have three oculars depend on electrons passing through the specimen of water. Put one edge of a coverslip in the water drop, then slowly lower the opposite edge to the water. Put one drop of stain at one edge of the coverslip. Put a piece of paper towel at the opposite side of the coverslip. Allow the towel to absorb some water so that the stain will move under the coverslip. have two objective lenses 11. Base your answer to the following question on the diagram below of a microscope and on your knowledge of biology. 4) Add one drop of stain to a slide. Place a piece of onion epidermis on top of the stain. Use a piece of paper towel to absorb the stain. Drop a coverslip on the epidermis to flatten it out. Lift the coverslip and add a drop of water to the epidermis. Replace the coverslip. 14. A slide of human blood cells was observed in focus under the low-power objective of a compound light microscope that had clean lenses. When the microscope was switched to high power, the image was dark and fuzzy. Which parts of the microscope should be used to correct this situation? 1) 2) 3) 4) The highest possible magnification that can be obtained when using this microscope is 1) 40× 2) 100× 3) 400× 4) 4,000× 12. A coverslip should be used for preparing a 1) 2) 3) 4) frog for dissection solution of iodine for food testing wet mount of elodea (a simple plant) test to determine the pH of a solution Page 2 nosepiece and coarse adjustment diaphragm and ocular objective and fine adjustment diaphragm and fine adjustment 15. Base your answer to the following question on the diagram below and on your knowledge of biology. Which laboratory technique is illustrated in the diagram? 1) 2) 3) 4) testing a specimen for amino acids determining the pH of a specimen measuring the photosynthetic rate in a specimen preparing a wet mount of a specimen Page 3 Answer Key Microscopes and Microscopy Quiz 1. — enlarged — upside down — backward — more detail 2. 2 3. 3 4. 1 5. 3 6. 4 7. 1 8. 3 9. 1 10. 4 11. 3 12. 3 13. 3 14. 4 15. 4 Page 4