Download SNC 2D1 Cell Biology Lab Activity

Name:__________________
Partner:__________________
Date:__________________
SNC 2D1 Cell Biology Lab Activity
Part A: Examining Cell Structures
This activity is to be done in partners. Each partner is responsible for completing 1 of the 2 drawings. Place
the name on the drawing each individual completes.
Purpose: To investigate how cell structures of plant cells and animal cells differ
Materials:
- Microscope
- Prepared slide of Elodea leaf cells
- Prepared slide of Whitefish embryo
- Clear ruler with millimetre markings
Predictions:
1. Predict what cell structures you will see in both the plant and animal cells
2. Predict what cell structures you will see in only the plant cells or animal cells
Procedure:
1. Set up a microscope
2. Calculate the total magnification under low power objective.
3. Place a clear plastic ruler with mm markings on top of the stage of your microscope. Looking through
the lowest power objective, focus your image of the ruler. Accurately measure the diameter of field
of view for the lowest power objective in µm.
4. Place the slide of the Elodea leaf cells on the stage. Looking through the low power objective, focus
your image using the course and fine adjustment knobs and view the specimen.
5. Rotate the nosepiece of the microscope until the medium power objective clicks into place. Looking
through the medium power objective, focus your image using the course and fine adjustment knobs
and view the specimen.
6. Rotate the nosepiece of the microscope until the high power objective clicks into place. Looking
through the high power objective, focus your image using the course and fine adjustment knobs and
view the specimen.
7. Calculate the total magnification under high power objective.
8. Calculate the diameter of field of view under high power objective in µm.
9. Observe the specimen under high power objective. Make a biological drawing of one cell.
10. When you are done, turn the objective lens so that the lowest power objective is above the specimen
and remove the slide from the stage.
11. Repeat the procedure using the Whitefish embryo slide. (There is no need to repeat the calculations
since they will be the same).
Predictions, Calculations and Discussion Questions
1. Predict what cell structures you will see in both the plant and animal cells (T&I
1
/1).
Name:__________________
Partner:__________________
Date:__________________
2. Predict what cell structures you will see in only the plant cells or animal cells (T&I
3. Calculation the total magnification under low power objective (T&I
/1).
4. Measurement of diameter of field of view for the low power objective in µm (T&I
5. Calculation the total magnification under high power objective (T&I
/1).
/2).
/1).
6. Calculation of diameter of field of view for the high power objective in µm (T&I
/2).
7. Look back at the predictions you made at the beginning of this investigation. What structures
predicted were not seen through the microscope? Offer a possible explanation of why these structures
were not observable under the microscope (T&I
/2)?
8. Complete the following table (K&U
Cell Organelle
Found in Plant
Cell, Animal Cell,
Both or Neither
Nucleus
/6).
Function
Cytoplasm
Cell Wall
Chloroplast
Cell Membrane
2
Name:__________________
Partner:__________________
Date:__________________
9. Write a statement explaining the similarities and differences observed between the plant and animal
cell with regards to structural appearance and organelles present (T&I
/2).
Biological Drawings (C
/10)
Checklist for Drawings of Cells
Title and Name
Entire space on paper provided utilized
Drawings completed in pencil
Magnification recorded
Label for cell membrane (use of ruler)
Label for nucleus (use of ruler)
Label for cytoplasm (use of ruler)
Label for cell wall (use of ruler)
Stippling used for dark areas
Labels on the right hand side of diagram
Elodea Leaf
/0.5
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/0.5
/0.5
/0.5
Whitefish Embryo
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/0.5
/0.5
/0.5
/0.5
/0.5
/0.5
------------------------------/0.5
/0.5
Part B: Mitosis in Plant and Animal Cells
This activity is to be done in partners. Each partner is responsible for completing 2 of the 4 drawings. Be sure
to place the name on the drawings that each individual completes.
Purpose: To investigate how mitosis is the same and different in plant and animal cells
Materials:
 Micro-Slide Viewer
 Animal Mitosis Slides
 Plant Mitosis Slides
Procedure:
1. Obtain a micro-slide viewer and both plant and animal mitosis slides
2. View each of the 8 steps of mitosis on the slides and pay close attention to the important events that
occur in both plant and animal mitosis
3. Be sure to read the summary for each slide included in the booklets that accompany the slide. They
will help explain the process of mitosis in plants and animals.
4. Choose 2 of the 4 major stages of mitosis (Prophase, Metaphase, Anaphase and Telophase) for plants
cells and complete a PROPER biological drawing of the plant cell at each of the 2 chosen stages of
mitosis. Be sure to indicate the stages chosen on your drawings.
5. Complete PROPER biological drawings of the animal cell at the other 2 major stages of mitosis. Be
sure to indicate the stages chosen on your drawings.
3
Name:__________________
Partner:__________________
Date:__________________
Discussion Questions
1. Look at the slides for Early Prophase for both the plant and animal cell. Although this process is
referred to as Early Prophase in the booklets accompanying the slides, what is another name given
for this period of the cell cycle? List 3 events that occur at this stage of the cell cycle (K&U
/4).
2. State the 4 stages of mitosis and describe 2 major events that occur at each stage (K&U
/4).
3. Look at the slides for Late Telophase for both the plant and animal cell. How does this stage of
mitosis differ between plant and animal cells? Although this process is referred to as Late Telophase
in the booklets that accompany the slides, what is another name given for the events that are
illustrated in these slides (T&I
/5).
Biological Drawings (C
/10)
Checklist for Drawings of Cells
Title ( Mitotic Stage & Type of Cell) & Name
Used entire space provide on page
Drawings completed in pencil
Stippling used for dark areas
Neatness
Knowledge & Understanding
/14
Prophase
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Metaphase
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/0.5
/0.5
Thinking & Inquiry
4
/17
Anaphase
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/0.5
/0.5
Telophase
/0.5
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Communication
/20