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Name:__________________ Partner:__________________ Date:__________________ SNC 2D1 Cell Biology Lab Activity Part A: Examining Cell Structures This activity is to be done in partners. Each partner is responsible for completing 1 of the 2 drawings. Place the name on the drawing each individual completes. Purpose: To investigate how cell structures of plant cells and animal cells differ Materials: - Microscope - Prepared slide of Elodea leaf cells - Prepared slide of Whitefish embryo - Clear ruler with millimetre markings Predictions: 1. Predict what cell structures you will see in both the plant and animal cells 2. Predict what cell structures you will see in only the plant cells or animal cells Procedure: 1. Set up a microscope 2. Calculate the total magnification under low power objective. 3. Place a clear plastic ruler with mm markings on top of the stage of your microscope. Looking through the lowest power objective, focus your image of the ruler. Accurately measure the diameter of field of view for the lowest power objective in µm. 4. Place the slide of the Elodea leaf cells on the stage. Looking through the low power objective, focus your image using the course and fine adjustment knobs and view the specimen. 5. Rotate the nosepiece of the microscope until the medium power objective clicks into place. Looking through the medium power objective, focus your image using the course and fine adjustment knobs and view the specimen. 6. Rotate the nosepiece of the microscope until the high power objective clicks into place. Looking through the high power objective, focus your image using the course and fine adjustment knobs and view the specimen. 7. Calculate the total magnification under high power objective. 8. Calculate the diameter of field of view under high power objective in µm. 9. Observe the specimen under high power objective. Make a biological drawing of one cell. 10. When you are done, turn the objective lens so that the lowest power objective is above the specimen and remove the slide from the stage. 11. Repeat the procedure using the Whitefish embryo slide. (There is no need to repeat the calculations since they will be the same). Predictions, Calculations and Discussion Questions 1. Predict what cell structures you will see in both the plant and animal cells (T&I 1 /1). Name:__________________ Partner:__________________ Date:__________________ 2. Predict what cell structures you will see in only the plant cells or animal cells (T&I 3. Calculation the total magnification under low power objective (T&I /1). 4. Measurement of diameter of field of view for the low power objective in µm (T&I 5. Calculation the total magnification under high power objective (T&I /1). /2). /1). 6. Calculation of diameter of field of view for the high power objective in µm (T&I /2). 7. Look back at the predictions you made at the beginning of this investigation. What structures predicted were not seen through the microscope? Offer a possible explanation of why these structures were not observable under the microscope (T&I /2)? 8. Complete the following table (K&U Cell Organelle Found in Plant Cell, Animal Cell, Both or Neither Nucleus /6). Function Cytoplasm Cell Wall Chloroplast Cell Membrane 2 Name:__________________ Partner:__________________ Date:__________________ 9. Write a statement explaining the similarities and differences observed between the plant and animal cell with regards to structural appearance and organelles present (T&I /2). Biological Drawings (C /10) Checklist for Drawings of Cells Title and Name Entire space on paper provided utilized Drawings completed in pencil Magnification recorded Label for cell membrane (use of ruler) Label for nucleus (use of ruler) Label for cytoplasm (use of ruler) Label for cell wall (use of ruler) Stippling used for dark areas Labels on the right hand side of diagram Elodea Leaf /0.5 /0.5 /0.5 /0.5 /0.5 /0.5 /0.5 /0.5 /0.5 /0.5 Whitefish Embryo /0.5 /0.5 /0.5 /0.5 /0.5 /0.5 /0.5 ------------------------------/0.5 /0.5 Part B: Mitosis in Plant and Animal Cells This activity is to be done in partners. Each partner is responsible for completing 2 of the 4 drawings. Be sure to place the name on the drawings that each individual completes. Purpose: To investigate how mitosis is the same and different in plant and animal cells Materials: Micro-Slide Viewer Animal Mitosis Slides Plant Mitosis Slides Procedure: 1. Obtain a micro-slide viewer and both plant and animal mitosis slides 2. View each of the 8 steps of mitosis on the slides and pay close attention to the important events that occur in both plant and animal mitosis 3. Be sure to read the summary for each slide included in the booklets that accompany the slide. They will help explain the process of mitosis in plants and animals. 4. Choose 2 of the 4 major stages of mitosis (Prophase, Metaphase, Anaphase and Telophase) for plants cells and complete a PROPER biological drawing of the plant cell at each of the 2 chosen stages of mitosis. Be sure to indicate the stages chosen on your drawings. 5. Complete PROPER biological drawings of the animal cell at the other 2 major stages of mitosis. Be sure to indicate the stages chosen on your drawings. 3 Name:__________________ Partner:__________________ Date:__________________ Discussion Questions 1. Look at the slides for Early Prophase for both the plant and animal cell. Although this process is referred to as Early Prophase in the booklets accompanying the slides, what is another name given for this period of the cell cycle? List 3 events that occur at this stage of the cell cycle (K&U /4). 2. State the 4 stages of mitosis and describe 2 major events that occur at each stage (K&U /4). 3. Look at the slides for Late Telophase for both the plant and animal cell. How does this stage of mitosis differ between plant and animal cells? Although this process is referred to as Late Telophase in the booklets that accompany the slides, what is another name given for the events that are illustrated in these slides (T&I /5). Biological Drawings (C /10) Checklist for Drawings of Cells Title ( Mitotic Stage & Type of Cell) & Name Used entire space provide on page Drawings completed in pencil Stippling used for dark areas Neatness Knowledge & Understanding /14 Prophase /0.5 /0.5 /0.5 /0.5 /0.5 Metaphase /0.5 /0.5 /0.5 /0.5 /0.5 Thinking & Inquiry 4 /17 Anaphase /0.5 /0.5 /0.5 /0.5 /0.5 Telophase /0.5 /0.5 /0.5 /0.5 /0.5 Communication /20