Download Lyc-O-Mato Capsules

Natural, Year Round Protective
& Beautifying
Dietary Supplements
2 Clinical Studies
Joost Overeem, MSc,
MSc, , LycoRed Sàrl,
rl, Switzerland
Free Radicals Theory of Aging
Free Radicals are very reactive molecules, which
are formed as a result of:
• Necessary metabolic reactions
• UVB/UVA Radiation
• Ozone
• Exposure to smoke
• Air pollutants
Free radicals multiply through series of chain
reactions. They can damage polyunsaturated fatty
acids, membrane phospholipids, DNA and
proteins.
Overview
Sun Exposure & Skin Damage
• Solar radiation induces photo damages to
•
•
•
•
skin
Most of the damaging effects are attributed to
UV radiation (290-400 nm)
Application of sunscreens containing UVB &
UVA filters provides limited protection
A small amount of residual UV light
penetrates into the skin, triggering the
formation of free radicals
Such damage has been associated with age
related skin problems
Overview – Cont’d
Sun Exposure & Skin DamageDamage- Cont’d
Cont d
• It has been shown that just one day of
•
•
unprotected sun exposure can cause irreversible
skin damage.
It only takes small amounts of sun exposure,
such as walking to the car or mailbox to start
damage
Despite frequent appeals by physicians, skin
cancer associations and the media, most
consumers are not complying with the
recommendation for daily, year-round sunscreen
use.
Photooxidative Stress
UV
LIGHT
Reactive Oxygen
Species (ROS)
Damage
DNA LIPIDS PROTEINS
Antioxidants
Photooxidative Stress Disorders of Light-exposed
Tissues
Eye
• Cataract
• Age-Related Macular Degeneration
Skin
• Sunburn
• Skin Aging – wrinkles, discoloration
• Photosensitivity Disorders
• Skin Cancer
Overview
Supplements for YearYear-Round
Protection and Beauty
• Dietary Supplements, Foods, beverages and
cosmeceuticals are being marketed for their
Health and Beauty attributes, more than ever
• Studies have shown that Antioxidants, are
effective protectants against Oxidative
Stress, because of the ability to quench free
radicals.
• The Antioxidants can enhance health &
beauty of skin by increasing its density,
thickness and smoothness
Carotenoids in Skin Protection
Photo-protective Effects of Lycopene
& other Carotenoids
The Carotenoid Family
Carotenoids
• Compounds found in fruits, vegetables and green
plants
• Of more than 600 carotenoids in nature, only 20
are found in human plasma and tissues
• The most abundant carotenoids are Betacarotene, Lutein, Lycopene and Zeaxanthin
• Lycopene, is the dominant carotenoid in the
Western diet and most abundant in the body
• In the skin tissue, the main carotenoids are
Lycopene and Beta- Carotene
Lyc-O-Mato®
A Natural, Year Round
Photo-protective &
Beautifying Dietary Supplement
The Lyc-O-Mato® concept
• All natural extract of red, non GMO ripe
•
•
tomatoes
Its unique activity is based on a combination of
lycopene and a complex consisting of:
- tocppherols
- beta-carotene
- colorless carotenoids: phytoene & phytofluene
Patents are starting to be granted to the Lyc-OMato complex for its protection through oral
supplementation
Phytonutrient content in LYC-O-MATO®
6% - Standardized Tomato Extract
Phytosterols
0.7%
Tocopherols
(Vit E) 2%
Other carotenoids
0.3%
Phytofluene
0.7%
Phytoene
0.7%
Beta-carotene
0.2%
Lycopene
6%
Lyc-O-Mato® in clinical study
Lycopene Levels in Skin
After 12 Weeks of Supplementation
Photoprotective Effects of Tomato Extracts vs. Lycopene
36 Volunteers (skin type II)
Synthetic Lycopene
Lyc-O-Mato Capsules
Lyc-O-Mato Drink fortified with Phytoene and Phytoflene
12 Week Supplementation
Serum Levels of Lycopene, Phytoene, Phytofluene: HPLC
Total carotenoid Skin Level (Reflection spectrophotometry)
Erythema Formation (Δ
Δ-a-Value at 1.25 MED)
Sunburn Reaction to solar light simulator
W. Stahl, It.J.Vit. Nutri. Res. 03/24, Oct. 2003
Daily Intake During the Study
Carotenoids Lyc-O-Mato Lyc-O-mato Lycopene
(mg/d)
drink
capsules
synthetic
Lycopene
8.2
9.8
10.2
Phytoene
4.5
1.0
-
Phytofluene
3.2
0.9
-
Relative Sun-Protective Effects
Protection
% of Δ-a-value on day 0
Lyc-O-Mato Lyc-O-Mato
Drink
Capsules
Synthetic
Lycopene
100
75
50
25
0
Week: 0
4 12
0
4 12
0
4 12
Total Carotenoids in Skin
(nmol/g)
Carotenoid Skin Levels
Lyc-O-Mato Lyc-O-Mato
Drink
Capsules
0.3
Synthetic
Lycopene
0.2
0.1
0
Week: 0 4 12
0
4 12
0
4 12
Is it Lycopene Only?
Phytoene/Phytofluene
Serum Levels
Lyc-O-Mato Lyc-O-Mato
Drink
Capsules
Synthetic
Lycopene
Phytoene in Serum (µmol/L)
0.3
0.2
0.1
0
Week: 0 4 12
0
4 12
0
4 12
Why Phytofluene and Phytoene are
so beneficial?
Absorption Spectra
UV-B
UV-A
lycopene
Phytofluene and Phytoene
Features & Benefits:
• Major carotenoids in tomato and in tomato extracts
(not present in synthetic preparations)
• Absorb light in UVA and UVB respectably
• Both are colorless carotenoids
Summary of the results
• After 12 weeks of oral supplementation,
erythema formation with synthetic Lycopene,
was reduced by 25%, whereas the Lyc-OMato® and Lyc-O-Mato® Drink showed a
38% and 50% reduction, respectively
Conclusion
• Protection against UV light-induced erythema
by Lycopene is increased as a result of the
presence of the carotenoids phytoene and
phytofluene and other phyto-nutrients
naturally present in the Lyc-O-Mato® extract.
Conclusion (ct’d)
• Consumption of Lyc-O-Mato® containing
products increases plasma and cellular
antioxidant concentrations
• Intervention studies suggest that such
products can improve cell resistance to
oxidative DNA damage
• The increased protection is not provided
by Lycopene alone, but is a result of the
other carotenoids / phytonutrients
Carotenoids in Skin Health and Beauty
Short clinical pilot
Protection
Against Sunburn Cell Formation
Sunburn Cell Formation - Overview
• Repetitive exposure of human skin to UVB and
•
•
UVA radiation, results in morphological and
histological changes
These changes include:
- Epidermal thickening
- Sunburn Cell Formation
- Elastin Damage
- Dermal Inflammation
Sunburn cells, are apoptotic (dead) cells, which
are indicative of damage caused by UV
exposure
Effect of Dietary Supplementation of
LycLyc-O-Mato
Mato 6%; LycLyc-O-Mato 6% & Lutein +
Rosemary oil on Sunburn Cells Formation
Study Objective
• Determination of the efficacy of oral supplementation of LycLyc-O-Mato
 6% in preventing photophoto-aging, as measured by the histological
analysis of sunburn cells
Test Products
• Group #1:2 capsules of LycLyc-O-Mato
Mato 6% per day, (each capsule
containing 5 mg Lycopene)
• Group #2: 2 capsules of LycLyc-O-Mato
Mato 6% per day (each capsule
containing 5 mg Lycopene) + 1 capsule containing 5 mg lutein
lutein plus
5 mg Carnosic acid (Rosemary oil)
• Group #3: Placebo control capsules (containing Soybean oil))
Study Period
• 10 weeks of supplementation
Effect Of Dietary Supplementation StudyStudyCont’d
Cont d
Study Population
• 15 female and male subjects, 18 + years of age, with
skin Type Ⅰ and Ⅱ
Protocol
• Subjects were randomized into two balanced groups of 5
• Group #1 consumed the LycLyc-O-Mato
Mato 6%;
• Group #2 consumed Lyc
Lyc--O-Mato
Mato 6% plus Lutein & Carnosic acid
(Rosemary oil)
• Group #3 consumed the placebo.
• Subjects returned the clinic at the end of week 10 for
administration of 2 MED. Subjects returned 24 hours
later for 3mm punch biopsy for sunburn cell counts.
Protection Against Sunburn Cell Formation
Number of Sunburn Cells per Sq. mm
1200
1000
800
600
400
200
0
Placebo
Lyc-O-Mato 6%
Lyc-O-Mato 6%+
Lutein /Rosemary
Summary
-
Dietary antioxidants provide moderate protection against
UV-induced erythema (sunburn)
-
Carotenoids can be used as dietary supplements, enriched
functional foods, beverages and cosmeceuticals for Year
Round Protection & Beauty
-
For activity, doses of > 10 mg carotenoids/day are needed;
intervention > 6 wk
-
Antioxidant activity is the main mechanisms of protection
Antioxidants improve skin texture (smoothness, thickness)
both orally and topically
Dietary Supplementation
Skin Care Claims
• Protection against Environmental Stress
— free radicals, ozone, pollution
• Beautifying (Corrective) Effects —
enhanced skin moisturization, skin density,
thickness (collagen formation), elasticity and
smoothness
Typical Lyc-o-Mato® product
Lyc-O-Mato® 15mg
Lycored magyarországi
képviselete:
HSH-Chemie Kft.
Balla Katalin
Tel:06202526031
mail:[email protected]