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LIGHT VS ELECTRON MICROSCOPE
Light
Electron
Sizes seen:
0.2 m –1 mm
2 nm- 100 m
Objects seen:
frog’s eggs
Cells
Nuclei
Mitochondria
Lysosomes
Chloroplasts
Flagella
Cilia
Cell wall
Cell membrane
Ribosomes
Endoplasmic
reticulum
Golgi body
Proteins
Lipids
Viruses
Small molecules
+ Light micro.
Resolution:
0.2 m
d = 0.612/NA
0.1 nm (2000X)
Illumination:
Electrons
Light
Disadvantage: lower resolving power
dead specimens
Advantage:
Living specimens
greater resolving
Focus:
Illumination
Condenser lens
use vacuum &
Magnetic lenses
Types lenses:
glass
magnetic
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