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RESEARCH USE ONLY POLYCLONAL ANTIBODY Anti-Human GM-CSF Code No. JM-5100-100 Host Rabbit IgG Quantity 100 µg BACKGROUND: Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. Human GM-CSF is a 14.0 kDa protein containing 123 amino acid residues. PRODUCT: Supplied as 100 μg (0.5 mg/ml) affinity purified rabbit anti-human GM-CSF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide. IMMUNOGEN: Immunogen used was recombinant GM-CSF expressed in E. coli. PREPARATION: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human GM-CSF. The antibody was purified by affinity chromatography employing immobilized human GM-CSF matrix. APPLICATIONS: The antibody can be used for Western blot analysis (0.5-1 μg/ml), ELISA (2-3 μg/ml), and neutralization assays (1-5 μg/ml). However, the optimal conditions should be determined individually. STORAGE: Store the antibody at -20°C. For long-term storage, aliquot and freeze at -70°C. Avoid freeze/thaw cycles. INTENDED USE: For research use only. or therapeutic use. human Not for clinical diagnosis 4 H Constitution Way · Woburn, MA 01801 · Phone: 1.800.200.5459 · Fax: 781-939-6963 · www.mblintl.com