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cellgro® Cell Culture Media and Reagents Product Selection Guide Contents Classical Media CMRL 1066 Dulbecco’s Modification of Eagle’s Medium (DMEM) DMEM/Ham’s F-12 50/50 Mix (Mod.) F-12K Nutrient Mixture (Kaighn’s Mod.) Glasgow’s MEM Ham’s F-10 Medium Ham’s F-12 Medium Improved MEM (Richter’s Mod.) Iscove’s Modification of DMEM Leibovitz’s L-15 (Mod.) MCDB 131 McCoy’s 5A (Iwakata and Grace Modification) Medium 199 (Mod.) Minimum Essential Medium (MEM) Alpha Medium Minimum Essential Medium (MEM) RPMI 1640 Buffered Salt Solutions Dulbecco’s Phosphate Buffered Saline (DPBS) Phosphate Buffered Saline (PBS) Hanks’ Balanced Salt Solution (HBSS) Phosphate Buffered Saline (PBS) Hanks’ Balanced Salt Solution (HBSS) 5 7 7 11 12 12 13 13 14 14 15 15 15 16 17 18 20 23 25 26 26 26 26 Serum-Free and Speciality Media 31 32 33 33 33 33 34 34 35 35 36 36 36 Human and Animal Sera 39 43 45 45 45 46 46 46 47 47 47 cellgro COMPLETE™ insectagro DS2™ insectagro™ insectagro Sf9™ transfectagro™ Lymphocyte Separation Medium Polysucrose 400 Tryptic Soy Broth (TSB) cellgro® rhAlbumin cellgro® rhLactoferrin stemgro® AB2™ Neural Base Media stemgro® ANS™ Neural Supplement Fetal Bovine Serum Donor Horse Serum Human AB Serum Porcine Serum Charcoal-Stripped FBS Dialyzed FBS ES Cell Tested FBS Gamma-Irradiated FBS Low IgG FBS Tetracycline Negative FBS Cell Culture Reagents 49 51 51 52 52 52 56 57 58 58 58 59 Insect Media and Reagents 63 64 64 64 65 Microbiology and Molecular Biology 67 68 68 68 68 68 68 69 69 69 70 70 71 71 71 72 72 72 73 73 74 74 74 74 75 75 75 High Quality Water 77 79 79 79 L-glutamine glutagro™ MEM Amino Acids MEM Vitamins Antibiotics and Antimycotics Buffers Trypsin Non-Enzymatic Cell Dissociation Agents Growth Supplements Hybridoma Reagents Miscellaneous Reagents Grace’s Insect Basal Medium Hink’s TNM-FH Medium insectagro™ Reagents insectagro™ Serum-Free Media Miller’s LB Broth S.O.C Medium Terrific Broth Ampicillin Carbenicillin Kanamycin Sulfate Neomycin Sulfate Streptomycin Sulfate Tetracycline Hydrochloride X-Gal Isopropylthiogalactoside (IPTG) Phosphate Buffered Saline (PBS), 10x SSC, 20x, Liquid SSPE, 20x, Liquid TAE Buffer, 10x TBE Buffer, 10x, Liquid TE Buffer, 1x, Liquid Tris Buffered Saline, 10x, Liquid Tris-Hydrochloride Buffers, 1M EDTA, 0.5M, pH 8.0 Molecular Biology Grade Water Reagent Grade Water, 4L Cube MOPS Buffer SDS (Sodium Dodecyl Sulfate), 10% Sodium Acetate, 3M Sodium Chloride, 5M Distilled, Deionized Water USP/EP Certified Sterile WFI Quality Water Sterile WFI Quality, Cell Culture Grade Water CLASSICAL MEDIA Classical Media Classical Cell Culture Media CMRL 1066 DMEM DMEM/Ham’s F-12 50/50 Mix (Mod.) F-12K Nutrient Mixture (Kaighn’s Mod.) Glasgow’s MEM Ham’s F-10 Medium Ham’s F-12 Medium Improved MEM (Richter’s Mod.) Iscove’s Modification of DMEM Leibovitz’s L-15 (Mod.) MCDB 131 McCoy’s 5A (Iwakata and Grace Modification) Medium 199 (Mod.) Minimum Essential Medium (MEM) RPMI 1640 7 7 11 12 12 13 13 14 14 15 15 15 16 17 20 5 Classical Media CLASSICAL MEDIA Classical Media Introduction Growth Promotion and Cytotoxicity Corning cellgro products undergo a variety of tests in which functionality and toxicity are determined using growth-promotion analysis. Product performance is measured by harvest to plant ratios or fold increase according to predetermined specifications. Physiochemical Properties Liquid and powder products undergo testing to determine pH and osmolality. Powders are also tested for residual moisture. The pH and osmolality tests are performed according to specific protocols in which all equipment is calibrated using standards which are traceable to the National Institute of Standards and Technology. Biological Tests Testing of cellgro products guarantee low levels of endotoxin, mycoplasma and an absense of contamination. Endotoxin: Media are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Chromogenic Assay. For product release, each lot of standard liquid media must have an endotoxin level of < 0.25 EU/mL. Powder products must have an endotoxin level of < 0.25 EU/mL when reconstituted to formula weight with low-endotoxin water. Sterility: All cellgro products are sterility tested. Mycoplasma: Media products are tested for mycoplasma offsite using a large-volume method described by Kern and Barile in “Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques,” M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11,5. Razin and J.G. Tulley, eds., New York: Academic Press, 159-165. 6 Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Classical Media CMRL 1066 CMRL 1066 is a modified version of Medium 199 and was designed initially for used with L-strain cells in unsupplemented culture. It has been used to maintain monolayer growth of permanent cell lines for extended periods of time without protein supplementation. Although developed for use in serum-free cell culture, CMRL 1066 can be supplemented with serum and used to support the growth of many types of cells. Without L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-114 15-110-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 36 Months Dulbecco’s Modification of Eagle’s Medium (DMEM) Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. 45000-306 45000-304 45000-310 45000-308 45000-334 45000-332 45000-314 45000-312 L-glutamine ¢ ¢ ¢ Sodium pyruvate ¢ ¢ Phenol red ¢ ¢ ¢ ¢ ¢ L-Cystine and L-Methionine ¢ ¢ ¢ ¢ ¢ Phosphate ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ 97060-876 Contains 45000-318 45000-316 45000-322 45000-320 45000-326 45000-324 ¢ HEPES High glucose ¢ 45000-330 45000-328 45000-340 45000-336 45000-342 Sodium pyruvate ¢ ¢ ¢ ¢ Phenol red ¢ ¢ L-Cystine and L-Methionine ¢ Phosphate ¢ HEPES ¢ High glucose ¢ Contains L-glutamine ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ 7 Classical Media CLASSICAL MEDIA Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose, L-glutamine, and sodium pyruvate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-304 10-013-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-306 10-013-CM 1L 6 15-30ºC 2-8ºC 18 Months 45000-308 10-013-LX 10L 1 15-30ºC 2-8ºC 18 Months 45000-310 10-013-LB 20L 1 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 1.0 g/L glucose, sodium pyruvate, L-glutamine and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-332 45000-334 Size Qty/Pk Shipping Storage Expires 10-014-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 10-014-CM 1L 6 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose and L-glutamine, without sodium pyruvate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-312 45000-314 Size Qty/Pk Shipping Storage Expires 10-017-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 10-017-CM 1L 6 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose and sodium pyruvate without L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-316 15-013-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-318 15-013-CM 1L 6 15-30ºC 2-8ºC 18 Months 45000-320 15-013-LX 10L 1 15-30ºC 2-8ºC 18 Months 45000-322 15-013-LB 20L 1 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose, without L-glutamine and sodium pyruvate Intended Use: In Vitro Diagnostic 8 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-324 15-017-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-326 15-017-CM 1L 6 15-30ºC 2-8ºC 18 Months Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Classical Media Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose, sodium pyruvate, and 25 mM HEPES, without L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-328 45000-330 Size Qty/Pk Shipping Storage Expires 15-018-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 15-018-CM 1L 6 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose and sodium pyruvate, without L-glutamine, L-methionine and L-cystine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-340 17-204-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose, and sodium pyruvate, without L-glutamine and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-336 17-205-CV Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x With 4.5 g/L glucose and sodium pyruvate, without L-glutamine and phosphate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-342 17-206-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x Without glucose, L-glutamine, and sodium pyruvate Intended Use: In Vitro Diagnostic New VWR Cat. No. Corning Cat. No. 97060-876 17-207-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months glutagro™ DMEM Dulbecco’s Modification of Eagle’s Medium, 1x with glutagro supplement, 4.5 g/L glucose, sodium pyruvate and phenol red Intended Use: In Vitro Diagnostic New VWR Cat. No. Corning Cat. No. 71002-872 10-101-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months glutagro™ DMEM Dulbecco’s Modification of Eagle’s Medium, 1x with glutagro supplement, 4.5 g/L glucose and phenol red, without sodium pyruvate Intended Use: In Vitro Diagnostic New VWR Cat. No. Corning Cat. No. 71002-874 10-102-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months 9 Classical Media CLASSICAL MEDIA Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder 45000-518 45000-520 45000-522 45000-524 L-glutamine ¢ ¢ Sodium pyruvate ¢ Phenol red ¢ ¢ L-Cystine and L-Methionine ¢ Phosphate Contains 45000-526 45000-534 ¢ ¢ ¢ ¢ ¢ ¢ ¢ High glucose ¢ ¢ ¢ Pyridoxine HCl ¢ ¢ ¢ HEPES ¢ Sodium bicarbonate Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder With 4.5 g/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-518 50-003-PB 10L 1 15-30ºC 2-8ºC 36 Months 45000-520 50-003-PC 50L 1 15-30ºC 2-8ºC 36 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder With 4.5 g/L glucose and L-glutamine, without sodium bicarbonate and sodium pyruvate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-522 50-013-PB 10L 1 15-30ºC 2-8ºC 36 Months 45000-524 50-013-PC 50L 1 15-30ºC 2-8ºC 36 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder With 4.5 g/L glucose, without sodium bicarbonate, L-glutamine, sodium pyruvate and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-526 90-013-PB 10L 1 15-30ºC 2-8ºC 36 Months Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder Without sodium bicarbonate, glucose, L-glutamine, sodium pyruvate, and phenol red Intended Use: In Vitro Diagnostic 10 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-534 90-113-PB 10L 1 15-30ºC 2-8ºC 36 Months Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Classical Media DMEM/Ham’s F-12 50/50 Mix (Mod.) This 1:1 mixture of Dulbecco’s Modified Eagle Medium and Ham’s F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. Ham’s F-12 media is based on Ham’s F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. 71002-674 45000-344 45000-352 45000-350 L-glutamine ¢ ¢ Sodium pyruvate ¢ ¢ ¢ Phenol red ¢ ¢ ¢ Contains HEPES 45000-348 45000-346 45001-052 45000-540 45000-542 45000-536 45000-538 ¢ ¢ ¢ ¢ ¢ ¢ Sodium bicarbonate ¢ ¢ ¢ ¢ Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-344 10-090-CV 500 mL 6 15-30ºC 2-8ºC 12 Months 71002-674 10-090-CM 1L 6 15-30ºC 2-8ºC 12 Months Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) With L-glutamine, without phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-052 16-405-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) Without L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-346 15-090-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-348 15-090-CM 1L 6 15-30ºC 2-8ºC 18 Months Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) With L-glutamine and 15 mM HEPES Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-350 45000-352 Size Qty/Pk Shipping Storage Expires 10-092-CV 500 mL 6 15-30ºC 2-8ºC 12 Months 10-092-CM 1L 6 15-30ºC 2-8ºC 12 Months 11 Classical Media CLASSICAL MEDIA DMEM/Ham’s F-12 50/50 Mix (Mod.) Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) Without sodium bicarbonate and L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-536 90-091-PB 10L 1 15-30ºC 2-8ºC 36 Months Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) Without sodium bicarbonate, L-glutamine, and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-540 90-090-PB 10L 1 15-30ºC 2-8ºC 36 Months glutagro™ DMEM/F-12 Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix with glutagro Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 71002-876 10-103-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 36 Months F-12K Nutrient Mixture (Kaighn’s Mod.) A modification of Ham’s F-12, F-12K contains higher concentrations of amino acids, sodium pyruvate and other components than the original. It is formulated to support the growth of cells including differentiated rat and chicken cell types and primary human hepatocytes. F-12K Nutrient Mixture (Kaighn’s Mod.) 1x With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-354 10-025-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Glasgow’s MEM Glasgow’s MEM is a modification of MEM formulated to support the growth of BHK-21 cells. The medium was originally based on BME with the addition of 10% tryptose phosphate broth and two times the concentration of amino acids and vitamins. Glasgow’s MEM, Powder With L-glutamine, without sodium bicarbonate and tryptose phosphate broth Intended Use: In Vitro Diagnostic 12 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-544 50-004-PB 10L 1 15-30ºC 2-8ºC 36 Months Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Classical Media Ham’s F-10 Medium Ham’s F-10 is a classical media designed to support the growth of a variety of cell types, including HeLa, Chinese Hamster Overy (CHO), primary cells from tissue explants and various types of human diploid cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-356 10-070-CV Size Qty/Pk Shipping Storage Expires 500 m L 6 15-30ºC 2-8ºC 12 Months Ham’s F-12 Medium Ham’s F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham’s F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. This medium is also suitable for carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, and rat, rabbit, and chicken embryos. Ham’s F-12 Medium, 1x With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-358 10-080-CV 500 mL 6 15-30ºC 2-8ºC 12 Months 45000-360 10-080-CM 1L 6 15-30ºC 2-8ºC 12 Months Ham’s F-12 Medium, Powder With L-glutamine, without sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-550 50-040-PB 10L 1 15-30ºC 2-8ºC 36 Months 13 Classical Media CLASSICAL MEDIA Improved MEM (Richter’s Mod.) A modified forumulation of Minimal Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites. Improved MEM (Richter’s Mod.), 1x With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-362 10-024-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Improved MEM (Richter’s Mod.), 1x With L-glutamine, without phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-364 10-026-CV Iscove’s Modification of DMEM Iscove’s Modification of DMEM (IMDM) is a highly enriched synthetic medium optimized for rapidly proliferation cultures of high cell density. A modified form of Dulbecco’s Modified Eagle Medium (DMEM), Iscove’s contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types. 45000-368 45000-366 Contains 45000-370 L-glutamine ¢ Phenol red ¢ ¢ Sodium Pyruvate ¢ ¢ Pyridoxine HCl ¢ ¢ Sodium Bicarbonate ¢ ¢ Iscove’s Modification of DMEM, 1x, liquid With L-glutamine and 25 mM HEPES, without ß-thioglycerol and ß-mercaptoethanol Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-366 45000-368 Size Qty/Pk Shipping Storage Expires 10-016-CV 500 mL 6 15-30ºC 2-8ºC 12 Months 10-016-CM 1L 6 15-30ºC 2-8ºC 12 Months Iscove’s Modification of DMEM, 1x, liquid With 25 mM HEPES, without ß-thioglycerol, ß-mercaptoethanol, and L-glutamine Intended Use: In Vitro Diagnostic 14 VWR Cat. No. Corning Cat. No. 45000-370 15-016-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Leibovitz’s L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments. It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine. The formation of acid metabolite byproducts is prevented by replacing glucose with galactose and sodium pyruvate. Examples of usage include the propagation of HEP-2, monkey kidney cells and primary explants of embryonic and adult human tissue. Leibovitz’s L-15 supports established cell lines as well as primary explants of embryonic and adult human tissue. Leibovitz’s L-15 (Mod.) 1x With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-372 10-045-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Leibovitz’s L-15 (Mod.) 1x, powder With L-glutamine, without phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-562 50-045-PB 10L 1 15-30ºC 2-8ºC 12 Months MCDB 131 MCDB 131 was originally developed by the researchers Knedler and Ham for the lowprotein and serum free growth of human microvascular endothelial cells (HMVEC). Since its development, this medium has been used for this cell type and others such as liver, smooth muscle, cardiac myocytes. It is one of a group of MCDB media which were designed to provide a defined and optimally balanced nutritional environment for various mammalian cell types. MCDB 131, 1x Without L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-112 15-100-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months McCoy’s 5A (Iwakata and Grace Modification) McCoy’s 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells. Originally modified by Iwakata and Grace, this version of McCoy’s 5A includes increased levels of inositol and glucose and contains L-glutamine. (con’t) 15 Classical Media Leibovitz’s L-15 (Mod.) Classical Media CLASSICAL MEDIA McCoy’s 5A (Iwakata and Grace Modification) Contains 45000-374 45000-376 L-glutamine ¢ ¢ Phenol red ¢ ¢ HEPES ¢ Sodium Bicarbonate ¢ ¢ McCoy’s 5A (Iwakata and Grace Modification), 1x With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-374 10-050-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months McCoy’s 5A (Iwakata and Grace Modification), 1x With L-glutamine and 25 mM HEPES Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-376 10-051-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 18 Months Medium 199 (Mod.) Medium 199 is often used in vaccine production, virology, and for the culture of various nontransformed cells. It was originally developed as the result of efforts to create a fully defined, animal origin-free medium. Long-term use of this medium requires serum supplementation. All components are animal origin-free. Contains 45000-378 45000-570 45000-578 L-glutamine ¢ ¢ ¢ Phenol red ¢ ¢ ¢ Earle’s Salts ¢ ¢ 45000-574 ¢ Hanks’ Salts ¢ Sodium Bicarbonate ¢ ¢ Medium 199 (Mod.), 1x With Earle’s salts and L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-378 10-060-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months Medium 199 (Mod.), Powder With Earle’s salts, L-glutamine, sodium bicarbonate Intended Use: In Vitro Diagnostic 16 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-570 50-050-PB 10L 1 15-30ºC 2-8ºC 18 Months Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Classical Media Medium 199 (Mod.), Powder With Earle’s salts, without sodium bicarbonate, L-glutamine, and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-574 90-050-PB 10L 1 15-30ºC 2-8ºC 36 Months Medium 199 (Mod.), Powder With Hanks’ salts, L-glutamine, sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-578 50-051-PB 10L 1 15-30ºC 2-8ºC 36 Months Minimum Essential Medium (MEM) Alpha Medium This is an enriched variation of Minimum Essential Medium, containing all 21 normal amino acids, and 5 additional vitamins. Minimum Essential Medium (MEM) is a common cell culture medium developed from early work with Basal Medium Eagle (BME). It is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells. When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts. Contains 45000-300 L-glutamine ¢ Phenol red ¢ Nucleosides ¢ Sodium Pyruvate Sodium Bicarbonate 45000-302 45000-504 ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ Minimum Essential Medium (MEM) Alpha Medium, 1x With Earle’s salts, ribonucleosides, deoxyribonucleosides, and L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-300 10-022-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Minimum Essential Medium (MEM) Alpha Medium, 1x With Earle’s salts, without ribonucleosides, deoxyribonucleosides, and L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-302 15-012-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months Minimum Essential Medium (MEM) Alpha Medium, Powder With Earle’s salts and L-glutamine, without ribonucleosides, deoxyribonucleosides, and sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-504 50-012-PC 50L 1 15-30ºC 2-8ºC 36 Months 17 Classical Media CLASSICAL MEDIA Minimum Essential Medium (MEM) Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells. When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts. 45000-382 45000-380 Contains 45000-386 45000-384 45000-394 L-glutamine ¢ Phenol red ¢ ¢ ¢ L-Methionine ¢ ¢ ¢ Calcium and magnesium ¢ ¢ Sodium Bicarbonate ¢ ¢ 45000-388 ¢ ¢ ¢ ¢ Minimum Essential Medium (MEM), 1x With Earle’s salts and L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-380 45000-382 Size Qty/Pk Shipping Storage Expires 10-010-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 10-010-CM 1L 1 15-30ºC 2-8ºC 18 Months Size Qty/Pk Shipping Storage Expires Minimum Essential Medium (MEM), 1x With Earle’s salts without L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-384 15-010-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-386 15-010-CM 1L 1 15-30ºC 2-8ºC 18 Months Minimum Essential Medium (MEM), 1x With Earle’s salts, without L-glutamine and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-388 17-305-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months Minimum Essential Medium (MEM), 1x Without L-glutamine, calcium, and magnesium Intended Use: In Vitro Diagnostic 18 VWR Cat. No. Corning Cat. No. 45000-394 15-015-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Classical Media Minimum Essential Medium (MEM), Powder 45000-582 45000-584 Contains 45000-594 45000-596 45000-592 L-glutamine ¢ Phenol red ¢ ¢ ¢ Earle’s Salts ¢ ¢ ¢ 45000-586 ¢ Minimum Essential Medium (MEM), Powder With Earle’s salts and L-glutamine, without sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-582 50-010-PB 10L 1 15-30ºC 2-8ºC 36 Months 45000-584 50-010-PC 50L 1 15-30ºC 2-8ºC 36 Months Minimum Essential Medium (MEM), Powder With Earle’s salts, without L-glutamine, phenol red, and sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-586 90-009-PB 10L 1 15-30ºC 2-8ºC 36 Months Minimum Essential Medium (MEM), Powder With Earle’s salts, without L-glutamine and sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-592 51-010-PC 50L 1 15-30ºC 2-8ºC 36 Months Minimum Essential Medium (MEM), Powder With Earle’s salts, L-glutamine, and nonessential amino acids, without sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-594 50-011-PB 10L 1 15-30ºC 2-8ºC 36 Months 45000-596 50-011-PC 50L 1 15-30ºC 2-8ºC 36 Months 19 Classical Media CLASSICAL MEDIA RPMI 1640 Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy’s 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse hybridomas, human leukocytes, and B and T lymphocytes. RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells. Contains 45000-406 45000-414 45000-404 45000-602 45000-398 45000-412 45001-110 45000-408 45000-418 45000-416 45000-410 45000-604 L-glutamine ¢ ¢ ¢ Phenol red ¢ ¢ ¢ HEPES ¢ ¢ ¢ ¢ ¢ ¢ ¢ L-Cystine and L-Methionine ¢ ¢ ¢ ¢ ¢ Sodium Bicarbonate ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ ¢ RPMI 1640, 1x With L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-396 10-040-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-398 10-040-CM 1L 6 15-30ºC 2-8ºC 18 Months 45000-400 10-040-LX 10L 1 15-30ºC 2-8ºC 18 Months 45000-402 10-040-LB 20L 1 15-30ºC 2-8ºC 18 Months Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months RPMI 1640, 1x With glutagro supplement and phenol red Intended Use: In Vitro Diagnostic New VWR Cat. No. Corning Cat. No. 71002-878 10-104-CV RPMI 1640, 1x Without L-glutamine Intended Use: In Vitro Diagnostic 20 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-404 15-040-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-406 15-040-CM 1L 6 15-30ºC 2-8ºC 18 Months 45000-408 15-040-LB 20L 1 15-30ºC 2-8ºC 18 Months Visit www.vwr.com for additional product and technical information. CLASSICAL MEDIA Classical Media RPMI 1640, 1x Without L-glutamine and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-410 17-205-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months Size Qty/Pk Shipping Storage Expires RPMI 1640, 1x With L-glutamine and 25 mM HEPES Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-412 10-041-CV 500 mL 6 15-30ºC 2-8ºC 18 Months 45000-414 10-041-CM 1L 6 15-30ºC 2-8ºC 18 Months Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 18 Months RPMI 1640, 1x With L-glutamine, without glucose Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-110 10-043-CV RPMI 1640, 1x With 25 mM HEPES, without L-glutamine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-418 15-041-CV RPMI 1640, 1x Without L-glutamine, L-methionine, and L-cystine Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-416 17-104-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 18 Months RPMI 1640, powder With L-glutamine, without sodium bicarbonate Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-602 50-020-PB 10L 1 15-30ºC 2-8ºC 36 Months 45000-604 50-020-PC 50L 1 15-30ºC 2-8ºC 36 Months 21 B U F F E R E D S A LT S O L U T I O N S Buffered Salt Solutions 25 26 26 23 Buffered Salt Solutions Dulbecco’s Phosphate Buffered Saline (DPBS) Phosphate Buffered Saline (PBS) Hanks’ Balanced Salt Solution (HBSS) B U F F E R E D S A LT S O L U T I O N S Buffered Salt Solutions Introduction Buffered Salt Solutions Physiochemical Properties Basal salt solutions undergo testing to determine pH and osmolality according to specific protocols which are in line with the National Institute of Standards and Technology. Biological Tests Corning cellgro prides itself on the ability to guarantee low levels of endotoxin, the absence of mycoplasma, and an absence of contamination. Endotoxin: Buffered salt solutions are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Assay, following guidelines outlined by the manufacturer and the FDA (1987). For release, each lot of buffered salt solutions must have an endotoxin level of ≤ 0.25 EU/mL. The majority of Corning cellgro’s liquid media are at or below our lowest detectable level today (≤ 0.001 EU/mL). Powder products must have an endotoxin level of ≤ 0.25 EU/mL when reconstituted to formula weight with low-endotoxin water. Sterility: Corning cellgro selects a number of samples from each production lot to test using the membrane filtration technique for 14 days, in accordance with USP XXIV Chapters <71> and <1211>. Mycoplasma: Buffered salt solution products are tested for mycoplasma off-site using large-volume method described by Kern and Barile in “Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques,” M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11,5. Razin and J.G. Tulley, eds., New York: Academic Press, 159-165. Dulbecco’s Phosphate Buffered Saline (DPBS) Dulbecco’s Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time. 24 45000-428 45000-432 45000-430 Contains 45000-426 Calcium ¢ ¢ Magnesium ¢ ¢ Potassium Chloride ¢ ¢ ¢ 45000-436 45000-434 45000-440 45000-438 ¢ Visit www.vwr.com for additional product and technical information. 45000-448 45000-446 45001-064 45000-442 ¢ ¢ B U F F E R E D S A LT S O L U T I O N S Dulbecco’s Phosphate Buffered Saline (DPBS) Dulbecco’s Phosphate Buffered Saline, 10x With calcium and magnesium VWR Cat. No. Corning Cat. No. 45000-426 20-030-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-30ºC 18 Months Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-30ºC 18 Months Dulbecco’s Phosphate Buffered Saline, 10x Without calcium and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-428 20-031-CV Dulbecco’s Phosphate Buffered Saline, 1x With calcium and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-430 21-030-CV 500 mL 6 15-30ºC 2-30ºC 36 Months 45000-432 21-030-CM 1L 6 15-30ºC 2-30ºC 36 Months Size Qty/Pk Shipping Storage Expires Dulbecco’s Phosphate Buffered Saline, 1x Without calcium and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-434 21-031-CV 500 mL 6 15-30ºC 2-30ºC 36 Months 45000-436 21-031-CM 1L 6 15-30ºC 2-30ºC 36 Months 45000-438 21-031-LX 10L 1 15-30ºC 2-30ºC 36 Months 45000-440 21-031-LB 20L 1 15-30ºC 2-30ºC 36 Months Dulbecco’s Phosphate Buffered Saline, Powder Without calcium and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-442 55-031-PB 10L 1 15-30ºC 2-8ºC 36 Months 45000-444 55-031-PC 50L 1 15-30ºC 2-8ºC 36 Months 25 Buffered Salt Solutions Intended Use: In Vitro Diagnostic B U F F E R E D S A LT S O L U T I O N S Phosphate Buffered Saline (PBS) Buffered Salt Solutions Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate. PBS helps to maintain a constant pH and is isotonic and non-toxic to cells. Phosphate Buffered Saline, 1x Without calcium and magnesium pH 7.4 ± 0.1 Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-446 45000-448 Size Qty/Pk Shipping Storage Expires 21-040-CV 500 mL 6 15-30ºC 2-30ºC 24 Months 21-040-CM 1L 1 15-30ºC 2-30ºC 24 Months Phosphate Buffered Saline, 10x Without calcium and magnesium pH 7.4 ± 0.1 Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-130 46-013-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 24 Months Hanks’ Balanced Salt Solution (HBSS) Hanks’ Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO2 atmospheric conditions. Contains 45000-450 45001-152 45000-454 45000-452 45000-460 45000-458 45000-464 45000-462 45001-101 45000-456 Calcium ¢ ¢ ¢ Magnesium ¢ ¢ ¢ Phenol red ¢ Sodium Bicarbonate ¢ ¢ ¢ ¢ 45000-470 45000-466 ¢ ¢ ¢ Hanks’ Balanced Salt Solution, 10x Without sodium bicarbonate, calcium, and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-450 20-021-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-30ºC 36 Months Hanks’ Balanced Salt Solution, 10x With calcium and magnesium, without phenol red and sodium bicarbonate Intended Use: In Vitro Diagnostic 26 VWR Cat. No. Corning Cat. No. 45001-152 20-023-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-30ºC 36 Months Visit www.vwr.com for additional product and technical information. B U F F E R E D S A LT S O L U T I O N S Hanks’ Balanced Salt Solution, 1x With calcium and magnesium Intended Use: In Vitro Diagnostic Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-452 21-020-CV 500 mL 6 15-30ºC 2-30ºC 36 Months 45000-454 21-020-CM 1L 6 15-30ºC 2-30ºC 36 Months Hanks’ Balanced Salt Solution, 1x With calcium and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-456 21-023-CV 500 mL 6 15-30ºC 2-30ºC 36 Months 45001-101 21-023-CV 1L 6 15-30ºC 2-30ºC 36 Months Size Qty/Pk Shipping Storage Expires Hanks’ Balanced Salt Solution, 1x Without calcium and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-458 21-021-CV 500 mL 6 15-30ºC 2-30ºC 36 Months 45000-460 21-021-CM 1L 6 15-30ºC 2-30ºC 36 Months Hanks’ Balanced Salt Solution, 1x Without calcium, magnesium, and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-462 45000-464 Size Qty/Pk Shipping Storage Expires 21-022-CV 500 mL 6 15-30ºC 2-30ºC 36 Months 21-022-CM 1L 6 15-30ºC 2-30ºC 36 Months Hanks’ Balanced Salt Solution, Powder Without sodium bicarbonate, calcium and magnesium Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-466 55-022-PB 10L 1 15-30ºC 2-8ºC 36 Months Hanks’ Balanced Salt Solution, Powder Without sodium bicarbonate, calcium, magnesium, and phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-470 55-021-PB 10L 1 15-30ºC 2-8ºC 36 Months 27 Buffered Salt Solutions VWR Cat. No. B U F F E R E D S A LT S O L U T I O N S Buffered Salt Solutions DPBS, PBS, and TBS Formulation 45000-426 45000-428 45000-430 45000-436 45000-434 45000-440 45000-438 10x, Liquid DPBS 10x, Liquid DPBS 1x, Liquid DPBS g/L g/L CaCl2 (anhydrous) 1.00 KCl KH2PO4 VWR Cat. No. 45000-442 45000-444 45000-448 45000-446 45001-130 45001-132 1x, Liquid DPBS Powder DPBS 1x, Liquid PBS 10x, Liquid PBS 10x, Liquid TBS g/L g/L g/L g/L g/L g/L - 0.10 - - - - - 2.00 2.00 0.20 0.20 0.20 - 2.0 - 2.00 2.00 0.20 0.20 0.20 0.144 2.40 - MgCl2 • 6H2O 1.00 - 0.10 - - - - - NaCl 80.00 80.00 8.00 8.00 8.00 9.00 80.00 80.00 Na2HPO4 (anhydrous) Description Volume Inorganic Salts 11.50 11.50 - 1.15 1.15 0.795 14.40 - Na2HPO4 • 7H2O - - 2.1716 - - - - - Tris Ultrapure - - - - - - - 24.2 - - - - 7.4 ± 0.1 - - - 4.4 ± 0.3 6.5 ± 0.5 7.0 ± 0.1 7.4 ± 0.1 - 7.4 ± 0.1 7.4 ± 0.1 @ 1:10 24 - 26ºC @ 1:10 276 ± 30 @ 1:10 276 ± 30 @ 1:10 285 ± 15 276 ± 10 282 ± 15 300 ± 20 - - - - - - 9.55 - - - Specifications pH (before adjustment) Final pH Osmolality (mOsm) Grams of powder required to prepare 1L (1x solution) 28 Visit www.vwr.com for additional product and technical information. B U F F E R E D S A LT S O L U T I O N S Hank’s Balanced Salt Solution (HBSS) Formulation 45000-460 45000-458 Description 45001-152 45000-454 45000-452 45000-460 45000-458 45000-464 45000-462 45001-101 45000-456 10x, Liquid HBSS 10x, Liquid HBSS 1x, Liquid HBSS 1x, Liquid HBSS 1x, Liquid HBSS g/L g/L g/L g/L - 1.4 0.14 KCl 4.00 4.00 KH2PO4 0.60 Volume 45000-470 45000-466 1x, Liquid HBSS Powder HBSS Powder HBSS g/L g/L g/L g/L - - 0.14 - - 0.40 0.40 0.40 0.40 0.40 0.40 0.60 0.06 0.06 0.06 0.06 0.06 0.06 Buffered Salt Solutions VWR Cat. No. Inorganic Salts CaCl2 (anhydrous) MgSO4 (anhydrous) - 0.977 0.0977 - - 0.0977 - - NaCl 80.00 80.00 8.00 8.00 8.00 8.00 8.00 8.00 Na2HPO4 (anhydrous) 0.477 0.477 0.0477 0.0477 0.0477 0.0477 0.0477 0.0477 - - 0.35 0.35 0.35 0.35 - - D-Glucose 10.00 10.00 1.00 1.00 1.00 1.00 1.00 1.00 Phenol red, Na 0.10 - 0.01 0.01 - - 0.01 - Powder (g/L) 0.35 - - - - - 0.35 0.35 7.5% solution (mL/L) 4.67 - - - - - 4.67 4.67 NaHCO3 Other Add NaHCO3 Specifications - - - - - - - 6.6 ± 0.5 Final pH pH (before adjustment) 6.0 ± 1.0 4.75 ± 0.25 7.25 ± 0.15 7.2 ± 0.2 7.25 ± 0.15 7.25 ± 0.15 7.25 ± 0.15 7.2 ± 0.2 Osmolality (mOsm/kg) 275 ± 30 (1:10) 275 ± 30 (1:10) 275 ± 15 275 ± 15 271 ± 15 287 ± 15 282 ± 15 281 ± 15 - - - - - - 9.52 9.51 Grams of powder required to prepare 1L (1x solution) 29 S E R U M - F R E E A N D S P E C I A LT Y M E D I A Serum-Free and Speciality Media 32 33 33 33 33 34 34 35 35 36 36 36 31 Serum-Free and Specialty Media cellgro COMPLETE™ insectagro DS2™ insectagro™ insectagro Sf9™ transfectagro™ Lymphocyte Separation Medium Polysucrose 400 Tryptic Soy Broth (TSB) cellgro® rhAlbumin cellgro® rhLactoferrin stemgro® AB2™ Neural Base Media stemgro® ABS™ Neural Supplement S E R U M - F R E E A N D S P E C I A LT Y M E D I A Serum-Free and Speciality Media cellgro COMPLETE™ Serum-Free/Low-Protein Medium cellgro COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy’s 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1.0 g/L). cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along as suspension and adherent cell cultures. This formulation contains L-glutamine. Serum-Free and Specialty Media cellgro COMPLETE Serum-Free/Low-Protein Medium, 1x With L-glutamine and 1 g/L BSA Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-420 40-101-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months cellgro COMPLETE Serum-Free/Low-Protein Medium, 1x With L-glutamine and 1 g/L BSA, without phenol red Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-422 40-102-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months cellgro FREE™ Serum-Free/Low-Protein Medium Optimized for the cultivation of Chinese Hamster Ovary (CHO) cells, along with many derivatives of this parent line, cellgro FREE is a proprietary serum-free and protein-free growth medium that does not contain any hormones or growth factors. This medium supports superior growth and viability over long term passages in both adherent and suspension culture, and is formulated without L-glutamine. cellgro FREE Serum-Free/Protein-Free Medium, 1x Without L-glutamine Intended Use: Research Use Only, Not for IVD Use 32 VWR Cat. No. Corning Cat. No. 45000-424 40-200-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Visit www.vwr.com for additional product and technical information. S E R U M - F R E E A N D S P E C I A LT Y M E D I A insectagro DS2™ Serum-Free/Protein-Free Medium insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and maintenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression. At the optimal temperature range 22-24ºC, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension. Under these conditions, the cells require minimal adaptation to serum-free culture. insectagro DS2 Serum-Free/Protein-Free Medium Without L-glutamine Intended Use: Research Use Only, Not for IVD Use Corning Cat. No. 45000-474 13-402-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months insectagro Sf9™ Serum-Free/Protein-Free Medium insectagro Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. Sf9 cells are cultured in non-humidified, non-CO2 incubators at 27ºC (room temperature) and display both monolayer and suspension culture qualities. With their fast doubling times of 10-22 hours, Sf9 cells are easily scaled up to large cultures using bioreactors. This formulation contains L-glutamine. insectagro Sf9 Serum-Free/Protein-Free Medium With L-glutamine Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-054 13-410-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months transfectagro™ transfectagro reduced serum medium is designed to maximize the growth of a variety of cell types under reduced serum conditions, generally allowing for at least a 50% reduction in serum use with minimal adaptation. transfectagro also improves transient transfection efficiency in a variety of cell types. This is a chemically-defined, reduced serum formulation. transfectagro Reduced Serum Medium With lower levels of calcium and phenol red, and with HEPES Intended Use: In Vitro Diagnostic New VWR Cat. No. Corning Cat. No. 71002-816 40-300-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months 33 Serum-Free and Specialty Media VWR Cat. No. S E R U M - F R E E A N D S P E C I A LT Y M E D I A Lymphocyte Separation Medium Lymphocyte Separation Medium (LSM) was originally designed for the In Vitro isolation of lymphocytes from diluted whole blood. It is a sterile filtered, iso-osmotic polysucrose and diatrizoate solution with low viscosity and a density of 1.077-1.080 g/mL at 20ºC. Reagents: Lymphocyte Separation Medium is a sterile filtered solution containing 96.219 g/L of diatrizoic acid and 61.363 g/L polysucrose 400 at a density of 1.077 - 1.080 g/mL @ 20ºC. The solution has an osmolarity of 290 ± 20 mOsm and a pH of 7.5 ± 1.5. Sodium hydroxide is added to adjust pH. Serum-Free and Specialty Media Lymphocyte Separation Medium Density - 1.077 - 1.080 g/mL at 20ºC Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-726 45000-728 Size Qty/Pk Shipping Storage Expires 25-072-CI 100 mL 1 15-30ºC 15-30ºC 18 Months 25-072-CV 500 mL 1 15-30ºC 15-30ºC 18 Months Polysucrose 400 Polysucrose 400 is a synthetic polymer of sucrose and epichlorohydrin used for the density gradient separation of cells, cell membranes and virus cells. It can also be used to isolate different organelles. This nonionic compound is provided in powder form. Polysucrose 400, Powder Used for cell separation Intended Use: Research Use Only, Not for IVD Use 34 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-730 61-196-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months 45000-732 61-196-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months Visit www.vwr.com for additional product and technical information. S E R U M - F R E E A N D S P E C I A LT Y M E D I A Tryptic Soy Broth (TSB) Tryptic Soy Broth (TSB) is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. It is a sterile filtered, ready-to-use solution for the imulation of aseptic filling of liquids and sterility testing. Due to the rich nutrient base, TSB may be used in the cultivation of fastidious microorganisms. This growth medium complies with current USP requirements and is free of animal-sourced components, making it ideal for testing the sterility of animal-free biopharmaceutical processes. VWR Cat. No. Corning Cat. No. 45001-156 46-060-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 15-30ºC 12 Months Tryptic Soy Broth, Animal-Free, Powder Used in the cultivation of fastidious microorganisms. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45001-226 61-411-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months cellgro® rhAlbumin cellgro rhAlbumin is a chemically-defined recombinant human serum albumin (rhHSA). Having an essential and non-essential fatty acid profile, this is a powerful media supplement that outperforms plasma-derived human serum albumin (pHSA), bovine serum albumin (BSA) and FBS. Some prominent applications for cellgro rhAlbumin are as a supplement for CHO cell culture and for the culture of various other cell lines. This product is plant-derived and completely animal-origin free. cellgro rhAlbumin Recombinant human serum albumin Intended Use: Research Use Only VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 89133-078 62-450-RF 1g 1 15-30ºC -20-8ºC 12 Months Long Term -20ºC 35 Serum-Free and Specialty Media Tryptic Soy Broth, Animal-Free, Liquid pH 7.1 - 7.5 @ 20-25ºC Tested negative for mycoplasma Intended Use: Research Use Only, Not for IVD Use S E R U M - F R E E A N D S P E C I A LT Y M E D I A cellgro® rhLactoferrin cellgro rhLactoferrin is a recombinant human hololactoferrin specifically intended for use as a cell culture supplement. It is a multi-functional protein that is a potent cell growth factor with anti-apoptotic and anti-microbial properties. This product is plant-derived and completely animal-origin free. Serum-Free and Specialty Media Corning cellgro rhLactoferrin, Powder Recombinant human hololactoferrin Intended Use: Research Use Only VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 89133-080 61-451-RF 1g 1 15-30ºC -20- 8ºC N/A stemgro® AB2™ Neural Base Media stemgro AB2 Neural Base Media is designed for use with stemgro® ANS™ Neural Supplement. The main applications are the expansion and proliferation Neural Progenitor Cells, and the maintenance of Differentiated Neuronal Cells. This basal medium is free of animal and protein components. stemgro AB2 Neural Base Media VWR Cat. No. Corning Cat. No. 97060-260 40-400-CV Size Qty/Pk Shipping Storage Expires 500 mL 1 Ambient 2-8ºC 18 Months stemgro® ANS™ Neural Supplement stemgro ANS Neural Supplement is used with stemgro AB2 Neural Base Media as part of a complete formulation for the expansion and proliferation of Neural Progenitor Cells, and for the maintenance of Differentiated Neuronal Cells. stemgro ABS Neural Base Media 36 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 97060-262 25-040-CR 2 mL 5 -25-5ºC -25-5ºC 18 Months Visit www.vwr.com for additional product and technical information. HUMAN AND ANIMAL SERA Human and Animal Sera Fetal Bovine Serum Donor Horse Serum Human AB Serum Porcine Serum Charcoal-Stripped FBS Dialyzed FBS ES Cell Tested FBS Gamma-Irradiated FBS Low IgG FBS Tetracycline Negative FBS 43 45 45 45 46 46 46 47 47 47 Human and Animal Sera 39 HUMAN AND ANIMAL SERA Human and Animal Sera Introduction Our high-quality serum products complement our wide range of sterile filtered, low endotoxin classical cell culture media perfectly: Fetal Bovine Serum (FBS) is a sterile, light brown colored liquid separated from the blood of the bovine fetus. All material meets the approval of the U.S. Department of Agriculture and is obtained from sources free of BSE, Foot and Mouth Disease, and other reportable diseases pertaining to the species. U.S. Sourced: Our U.S. sourced Premium Fetal Bovine serum, with the lowest endotoxin and hemoglobin specifications, is collected from cattle herds only within the United States. Also available heat-inactivated. Human and Animal Sera USDA: The USDA-certified Fetal Bovine Serum, also low in endotoxin and hemoglobin, is derived from USDA-certified facilities outside the United States, primarily Mexico. Mexico is recognized by the United States Department of Agriculture (USDA) to be free of bovine spongiform encephalopathy (BSE) and Foot and Mouth Disease (FMD). This is also available heat-inactivated. Donor Calf Serum is a sterile, light brown-colored liquid collected from herds of bovine calves located entirely in the United States. Animals are regularly inspected by licensed veterinarians and certified to be free of diseases pertaining to the species. Donor Horse Serum is a sterile, light brown-colored liquid collected from controlled standing herds of animals located entirely in the United States. Animals are inspected periodically by a licensed veterinarian. Diet and worming are strictly controlled and documented to ensure all animals are healthy. Each lot of Donor Horse Serum is extensively tested, and must be negative for Equine Infectious Anemia (EIA) and Equine Viral Arteritis (EVA). Chemical constituents are measured further to ensure lot-to-lot consistency. Porcine Serum is a sterile, light-brown liquid, collected from healthy pigs of U.S.-Origin, and free of Foot and Mouth Disease, Rinderpest, and Bovine Spongiform Encephalopathy. Each lot undergoes a biochemical analysis to ensure consistency. Specialty Serum products are also offered by Corning cellgro for a variety of product applications. All sera is separated from the collected blood by refrigerated centrifugation, true-pooled, filtered, and immediately frozen. The filtration process consists of a series of three 0.1 micron sterilizing filters, followed by a final “polishing” filter prior to final packaging. Only raw serum that meets our strict specifications for endotoxin, residual hemoglobin, pH and microbial quality is approved for production. 40 Visit www.vwr.com for additional product and technical information. HUMAN AND ANIMAL SERA Serum Product Specifications Fetal Bovine Serum, Premium VWR Cat. No. Size Qty/Pk 45001-106 500 mL 1 45001-108 500 mL 1 Endotoxin ≤ 20 EU/mL Hemoglobin ≤ 30 mg/dL Total Protein 40 ± 10 g/L pH 7.8 ± 0.8 Osmolality 315 ± 35 mOsm/kg Sterility Testing (current USP) Sterile Virus Testing [9CFR 113.53] Mycoplasma Negative Fetal Bovine Serum, Regular Size Qty/Pk 45000-734 500 mL 1 45000-736 500 mL 1 Endotoxin ≤ 20 EU/mL Hemoglobin ≤ 30 mg/dL Total Protein 40 ± 10 g/L pH 7.6 ± 0.8 Osmolality 315 ± 35 mOsm/kg Sterility Testing (current USP) Sterile Virus Testing [9CFR 113.53] Mycoplasma Negative Human and Animal Sera VWR Cat. No. Donor Calf Serum VWR Cat. No. Size 45001-060 Qty/Pk 500 mL Endotoxin 1 ≤ 5.0 EU/mL Hemoglobin ≤ 20 mg/dL Total Protein 6.0 ± 2.0 g/dL pH 7.8 ± 0.8 Osmolality 315 ± 35 mOsm/kg Sterility Testing (current USP) Sterile Virus Testing [9CFR 113.53] Mycoplasma Negative 41 HUMAN AND ANIMAL SERA Serum Product Specifications Donor Horse Serum VWR Cat. No. 45001-058 Size Qty/Pk 500 mL 1 Endotoxin (LAL) ≤ 5.0 EU/mL Hemoglobin ≤ 20 mg/dL Total Protein 6.0 ± 2.0 g/dL pH 7.8 ± 0.8 Osmolality 315 ± 35 mOsm/kg Sterility Testing (current USP) Sterile Virus Testing [9CFR 113.53] Mycoplasma Negative Human and Animal Sera Human AB Serum VWR Cat. No. 45001-062 Size Qty/Pk 100 mL 1 Endotoxin (LAL) ≤ 5.0 EU/mL Hemoglobin ≤ 20 mg/dL Total Protein 6.0 ± 2.0 g/dL pH 7.8 ± 0.8 Osmolality 315 ± 35 mOsm/kg Sterility Testing (current USP) Sterile Virus Testing [9CFR 113.53] Mycoplasma Negative 42 Visit www.vwr.com for additional product and technical information. HUMAN AND ANIMAL SERA Fetal Bovine Serum Fetal Bovine Serum (FBS) is the most common supplement used in cell culture. Its use at specific concentrations provides many compounds that have been shown to satisfy specific metabolic requirements for the culture of cells, including various growth promoting factors, along with a variety of undefined components. Fetal Bovine Serum, Premium Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-106 35-015-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Fetal Bovine Serum, Premium (Heat Inactivated) Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL Intended Use: Research Use Only, Not for IVD Use Corning Cat. No. 45001-108 35-016-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Gamma-Irradiated Fetal Bovine Serum, Premium Used in virus and vaccine production, projects needing a minimized risk of virus and Human and Animal Sera VWR Cat. No. mycoplasma contamination along with very low endotoxin. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-300 35-070-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Dialyzed Fetal Bovine Serum, Premium Used in any application needing a more defined serum or a need for low endotoxin concentration. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-302 35-071-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Charcoal-Stripped Fetal Bovine Serum, Premium Used in immunoassays, hormone-receptor assays, low virus/endotoxin applications. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-304 35-072-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC 43 HUMAN AND ANIMAL SERA Fetal Bovine Serum Fetal Bovine Serum, Regular Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-734 35-010-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Fetal Bovine Serum, Regular (Heat Inactivated) Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-736 35-011-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC IgG Stripped Fetal Bovine Serum, Premium Human and Animal Sera Used in commercial antibody production, specialized organism propagation. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-306 35-073-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Embryonic Stem Cell Tested FBS, Premium Used in embryonic stem cell research (validated for mouse but suitable for human) Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-308 35-074-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Tetracycline Negative Fetal Bovine Serum, Premium Used in cell culture applications using tet-inducible gene expression systems, any other application in which the presence of tetracycline would prove disruptive. Intended Use: Research Use Only, Not for IVD Use 44 VWR Cat. No. Corning Cat. No. 97065-310 35-075-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Visit www.vwr.com for additional product and technical information. HUMAN AND ANIMAL SERA Donor Calf Serum, U.S. Sourced Donor Calf Serum is collected from bovine calves less than eight months of age. The serum is separated from the blood by a mechanical defibrination process. Donor calf serum can be used for cells which have shown especially rapid growth with fetal bovine serum but is not the best choice for suspension cultures because of the turbidity associated with its use, which could complicate cell counting and viability measurements. Donor Calf Serum, U.S. Sourced Hemoglobin ≤ 20 mg/dL Endotoxin ≤ 5.0 EU/mL Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-060 35-022-CV Size Qty/Pk Shipping Storage Expires 500 mL 1 -15-25ºC -15-25ºC up to 5 years Donor Horse Serum, U.S. Sourced Human and Animal Sera Donor Horse Serum is serum collected aseptically from healthy equines. The serum is separated aseptically from the blood of the animal and allowed to spontaneously clot. Horse serum can be combined with fetal bovine serum for the culture of rat adrenal pheochromocytoma (PC12) cells, and is often used in testing media for mycoplasma contamination. Donor Horse Serum, U.S. Sourced Hemoglobin ≤ 20 mg/dL Endotoxin ≤ 5.0 EU/mL Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-058 35-030-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Human AB Serum Human AB Serum is processed from donor plasma collected at U.S. centers. Each donor unit of plasma is negative for Hepatitis B (HBsAg), Hepatitis C (anti-HCV), Human Immunodeficiency Virus-1 (HIV-1), Human Immunodeficiency Virus-2 (HIV-2), Human Immunodeficiency Virus antibody (HIV-1Ag), and ALT by FDA approved methods. Note: Proper safety precautions for potentially infectious agents must be taken despite these assurances. Human AB Serum Hemoglobin ≤ 20 mg/dL Endotoxin ≤ 5.0 EU/mL Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. 45001-062 Corning Cat. No. Size Qty/Pk Shipping Storage Expires 35-060-CI 100 mL 1 -15-25ºC -15-25ºC 24 Months 45 HUMAN AND ANIMAL SERA Specialized Serum Charcoal-Stripped FBS Charcoal-Stripped FBS has been subjected to charcoal filtration, which removes liphilic compounds (hormones, certain growth factors and cytokines) and virus particles. Charcoal-Stripped FBS is often used in immunoassays and hormone studies. Used in immunoassays and hormone studies. Charcoal or charcoal-dextran removes lipophilic compounds (hormones, certain growth factors and cytokines) and virus particles. Human and Animal Sera Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-304 35-072-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Dialyzed FBS Fetal bovine serum contains a wide variety of biological molecules and compounds which can influence cell development. This serum is dialyzed against physiological saline to remove small (less than 10kDa) potential contaminants while preserving the biological integrity of the final product. An extra filtration step of 0.1 micron further ensures purity. Dialyzed FBS is ideal for any application needing a more defined serum or a need for low endotoxin concentration. Used in any application needing a more defined serum or a need for low endotoxin concentration. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-302 35-071-CV Size Qty/Pk Shipping Storage Expires 500 mL 1 -15-25ºC -15-25ºC 36 Months ES Cell Tested FBS, U.S. Origin ES Cell Tested FBS meets the specialized needs of Embryonic Stem Cells (ESC’s). These cells require a strictly controlled environment to minimize growth factor and cytokineincuded differentiation. Embryonic stem cell qualified FBS is stringently validated for its ability to support mouse ESC colony formulation and proper cell morphology while allowing for minimal differentiation. Used in embryonic stem cell research (validated for mouse but suitable for human). Intended Use: Research Use Only, Not for IVD Use 46 VWR Cat. No. Corning Cat. No. 97065-300 35-070-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Visit www.vwr.com for additional product and technical information. HUMAN AND ANIMAL SERA Gamma-Irradiated FBS Gamma irradiation provides a method for complete viral and mycoplasma deactivation while maintaining the biological activity of the final product. Gamma-irradiated serum is useful for projects requiring very low endotoxin and a minimized risk of virus and mycoplasma contamination. Used in virus and vaccine production, projects needing a minimized risk of virus and mycoplasma contamination along with very low endotoxin. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-300 35-070-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Low IgG FBS Used in commercial antibody production, specialized organism propagation. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-306 35-073-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC Tetracycline Negative FBS, U.S. Origin Tetracycline Negative FBS is tested for the presence of tetracycline, an antibiotic that is frequently found in sera. Researchers using very sensitive tet-inducible gene expression systems encounter problems with unintended expression as a result of the presence of tetracycline in serum. In these cases, serum containing minimal levels of tetracycline can help ensure accurate gene expression. The tetracycline test is preformed either with the ELISA method or bioassay methodology techniques. The limit of detection is approximately 0.1 µg/ml. Used in cell culture applications using tet-inducible gene expression systems, any other application in which the presence of tetracycline would prove disruptive. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 97065-310 35-075-CV Size Qty/Pk Shipping Storage 500 mL 1 -15-25ºC -15-25ºC 47 Human and Animal Sera Low IgG FBS has been tested for the presence of IgG antibodies to meet strict specifications. It is useful in IgG antibody production, in the propagation of organisms that are normally hindered by the presence of IgGs, and any project in which downstream results would be hindered by exogenous IgGs. C E L L C U LT U R E R E A G E N T S Cell Culture Reagents L-glutamine glutagro™ MEM Amino Acids MEM Vitamins Antibiotics and Antimycotics Buffers Trypsin Non-Enzymatic Cell Dissociation Agents Growth Supplements Hybridoma Reagents Miscellaneous Reagents 51 51 52 52 52 56 57 58 58 58 59 Cell Culture Reagents 49 C E L L C U LT U R E R E A G E N T S Cell Culture Reagents Introduction Growth media is used either for cell culture or microbiological culture. These require nutrients, supplements, buffers and other reagents specific to the product being grown to maximize results and consistency. There are two major types of growth media: those used for cell culture, which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. Cell Culture Reagents The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellularparasites and require a growth medium composed of living cells. 50 Visit www.vwr.com for additional product and technical information. C E L L C U LT U R E R E A G E N T S Amino Acids and Vitamins L-glutamine L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing compounds toxic to cells. Optimal cell performance usually requires supplementation of the media with Lglutamine prior to use. This formulation is prepared in cell culture grade water. L-glutamine, Liquid 200 mM solution (29.20 mg/mL with 8.5 g/L NaCl) Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-676 25-005-CI 100 mL 6 -15-25ºC -15-25ºC 24 Months 45000-678 25-005-CV 500 mL 6 -15-25ºC -15-25ºC 24 Months L-glutamine, Powder Molecular weight 146.15 Intended Use: In Vitro Diagnostic Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-680 61-030-RM 100 g 1 15-30ºC 2-8ºC 12 Months 45000-682 61-030-RO 500 g 1 15-30ºC 2-8C 12 Months 45000-684 61-030-RR 1 kg 1 15-30ºC 2-8C 12 Months glutagro™ L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as frozen solution but degrades rapidly in liquid media or stock solutions, producing toxin compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. glutagro is a stabilized dipeptide form of L-glutamine which does not degrade in liquid media during storage or incubation, providing superior results in several application. glutagro (Dipeptide L-glutamine), Liquid 200 mM solution with 8.5 g/L NaCl Intended Use: In Vitro Diagnostic New VWR Cat. No. Corning Cat. No. 45001-086 25-015-CI AMEM BME DMEM DMEM/F12 EMEM Isocove’s DMEM M199 RPMI 1640 Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 2-30ºC 24 Months 292 mg/L 292 mg/L 584 mg/L 365.10 mg/L 292 mg/L 584 mg/L 100 mg/L 292 mg/L Supplementation table for L-Glutamine and L-Alanyl-L-Glutamine to L-Glutamine-Deficient Culture Media 51 Cell Culture Reagents VWR Cat. No. C E L L C U LT U R E R E A G E N T S MEM Amino Acids MEM Amino Acids 50x solution without L-glutamine Allows for efficient addition to media with no risk of dilution of media components Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-698 25-030-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 36 Months MEM Nonessential Amino Acids 100x solution The addition of NEAA to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-700 25-025-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 18 Months MEM Vitamins Cell Culture Reagents MEM Vitamins 100x solution Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-702 25-020-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 24 Months Size Qty/Pk Shipping Storage Expires 50 mL 6 -15-25ºC -15-25ºC 18 Months Qty/Pk Shipping Storage Expires Antibiotics and Antimycotics Amphotericin B, Liquid 250 µg/mL solubilized Intended Use: Research Use Only VWR Cat. No. Corning Cat. No. 45000-808 30-003-CF Ampicillin, Sodium Salt Inhibits bacterial cell-wall synthesis Intended Use: Research Use Only 52 VWR Cat. No. Corning Cat. No. 45000-612 61-238-RH 10 g 1 15-30ºC 2-8ºC ≥ 12 Months 45000-614 61-238-RM 100 g 1 15-30ºC 2-8ºC ≥ 12 Months Size Visit www.vwr.com for additional product and technical information. C E L L C U LT U R E R E A G E N T S Antibiotics and Antimycotics Antibiotic-Antimycotic Solution 10,000 I.U. Penicillin (per mL), 10,000 µg/mL Streptomycin, 25µg/mL Amphotericin with 8.5 g/L NaCl Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-616 30-004-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 18 Months Blasticidin S HCl, Powder Inhibits translation in prokaryotes and eukaryotes Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 71002-676 30-100-RB Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 18 Months Chloramphenicol, Powder Prototypical broad spectrum antibiotic Intended Use: Research Use Only, Not for IVD Use Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-618 61-239-RI 25 g 1 15-30ºC 15-30ºC ≥ 12 Months Cell Culture Reagents VWR Cat. No. Ciprofloxacin Hydrochloride, Powder A fluoroquinolone antibiotic Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-620 61-277-RG 1g 1 15-30ºC 15-30ºC ≥ 12 Months 45000-622 61-277-RG 5g 1 15-30ºC 15-30ºC ≥ 12 Months G418 Sulfate, Liquid 50 mg/mL solution Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-630 45001-076 Size Qty/Pk Shipping Storage Expires 30-234-CR 20 mL 1 15-30ºC 2-8ºC ≥ 12 Months 30-234-CI 100 mL 1 15-30ºC 2-8ºC ≥ 12 Months 53 C E L L C U LT U R E R E A G E N T S Antibiotics and Antimycotics G418 Sulfate, Powder Potency > 700 µg/mg Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-624 61-234-RF 1g 1 15-30ºC 15-30ºC ≥ 12 Months 45000-626 61-234-RG 5g 1 15-30ºC 15-30ºC ≥ 12 Months 45000-628 61-234-RK 50 g 1 15-30ºC 15-30ºC ≥ 12 Months Gentamicin Sulfate, Liquid Broad spectrum antibiotic recommended at 50 µg/mL Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-636 30-005-CR Size Qty/Pk Shipping Storage Expires 10 mL 10 15-30ºC 15-30ºC 12 Months Gentamicin Sulfate, Powder Broad spectrum antibiotic recommended at 50 µg/mL Cell Culture Reagents Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-632 45000-634 Size Qty/Pk Shipping Storage Expires 61-098-RA 100 mg 1 15-30ºC 2-8ºC ≥ 12 Months 61-098-RF 1g 1 15-30ºC 2-8ºC ≥ 12 Months Hygromycin B Solution Blocks polypeptide synthesis and inhibits elongation Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-806 30-240-CR Size Qty/Pk Shipping Storage Expires 20 mL 1 15-30ºC 2-8ºC 18 Months Kanamycin Sulfate, Liquid Inhibits protein synthesis Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-810 30-006-CF Size Qty/Pk Shipping Storage Expires 50 mL 6 -15-25ºC -15-25ºC 18 Months Kanamycin Sulfate, Powder Inhibits protein synthesis Intended Use: Research Use Only, Not for IVD Use 54 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-640 61-176-RG 5g 1 15-30ºC 15-30ºC 18 Months Visit www.vwr.com for additional product and technical information. C E L L C U LT U R E R E A G E N T S Antibiotics and Antimycotics Neomycin Sulfate, Powder Inhibits initiation and elongation during protein synthesis Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-644 61-241-RG 5g 1 15-30ºC 15-30ºC 18 Months Penicillin-Streptomycin Solution, 50x 5,000 I.U. penicillin (per mL), 5,000 µg/mL streptomycin Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-650 30-001-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 18 Months Penicillin-Streptomycin Solution, 100x 10,000 I.U. penicillin (per mL), 10,000 µg/mL streptomycin Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-652 30-002-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 12 Months Penicillin-Streptomycin-L-Glutamine, 100x VWR Cat. No. Corning Cat. No. 45001-103 30-009-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 12 Months Puromycin Dihydrochloride, Powder A broad-spectrum aminonucleoside antibiotic Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 62111-170 61-385-RA Size Qty/Pk Shipping Storage Expires 100 mL 1 -15-25ºC -15-25ºC 12 Months Streptomycin Sulfate, Powder An aminoglycoside antibiotic Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-654 61-088-RM 5g 1 2-8ºC 2-8ºC 12 Months Tetracycline Hydrochloride, Powder Broad spectrum antibiotic Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-656 61-242-RG 5g 1 2-8ºC 2-8ºC 12 Months 55 Cell Culture Reagents 10,000 I.U. penicillin (per mL), 10,000 µg/mL streptomycin, 29.2 mg/mL L-glutamine Intended Use: Research Use Only, Not for IVD Use C E L L C U LT U R E R E A G E N T S Buffers HEPES, Liquid 1M solution (238.3 mg/mL) Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-690 25-060-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 15-30ºC 24 Months HEPES, Powder Molecular Weight 238.3 Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-692 61-034-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months 45000-694 61-034-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months 45000-696 61-034-RR 1 kg 1 15-30ºC 15-30ºC ≥ 12 Months Sodium Bicarbonate, Liquid Commonly used to maintain physiological pH of 7.2-7.4 Cell Culture Reagents Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-704 25-035-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 15-30ºC 36 Months Sodium Bicarbonate, Powder Molecular Weight 84.01 Intended Use: In Vitro Diagnostic 56 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-706 61-065-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months 45000-708 61-065-RR 1 kg 1 15-30ºC 15-30ºC ≥ 12 Months Visit www.vwr.com for additional product and technical information. C E L L C U LT U R E R E A G E N T S Trypsin Trypsin, 1x 0.25% trypsin in HBSS, without calcium and magnesium Porcine parvovirus tested Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-658 25-050-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 12 Months Trypsin EDTA, 1x 0.05% trypsin/0.53 mM EDTA in HBSS, with sodium bicarbonate, calcium, and magnesium Porcine Parvovirus Tested Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-082 25-051-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 12 Months Trypsin EDTA, 1x 0.05% trypsin/0.53 mM EDTA in HBSS, without sodium bicarbonate, calcium and magnesium Porcine parvovirus tested Intended Use: In Vitro Diagnostic Corning Cat. No. 45001-660 45001-662 Size Qty/Pk Shipping Storage Expires 25-052-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months 25-052-CV 500 mL 6 -15-25ºC -15-25ºC 12 Months Trypsin EDTA, 1x 0.25% trypsin/2.21 mM EDTA in HBSS, without sodium bicarbonate, calcium, and magnesium Porcine Parvovirus tested Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-664 25-053-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 12 Months Trypsin, 10x 2.5% trypsin in HBSS without calcium, magnesium, and phenol red Porcine parvovirus tested Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-666 25-054-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 -15-25ºC -15-25ºC 12 Months 57 Cell Culture Reagents VWR Cat. No. C E L L C U LT U R E R E A G E N T S Non-Enzymatic Cell Dissociation Agents Cellstripper™ Cellstripper is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper achieves superior results in comparison to enzymatic dissociation products. Cellstripper, Liquid Non-enzymatic cell dissociation solution Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-668 25-056-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 15-30ºC 12 Months Growth Supplements Insulin-Transferrin-Selenium (ITS), 100x For use in adherent (anchorage-dependent) cultures Cell Culture Reagents Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-090 25-800-CR Size Qty/Pk Shipping Storage Expires 10 mL 1 2-8ºC 2-8ºC 18 Months Hybridoma Reagents HAT (Hypoxanthine, Aminopterin, Thymidine), 50x Hypoxanthine, aminopterin, and thymidine (HAT) media is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45000-686 25-046-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 -15-25ºC -15-25ºC 28 Months HT (Hypoxanthine, Thymidine). 50x Hypoxanthine and thymidine (HT) media is a “rescue media” that provides preformed purines and pyridmidines to overcome the effects of residual intracellular aminopterin Intended Use: In Vitro Diagnostic 58 VWR Cat. No. Corning Cat. No. 45000-688 25-047-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 2-8ºC 20 Months Visit www.vwr.com for additional product and technical information. C E L L C U LT U R E R E A G E N T S Miscellaneous Reagents Dimethyl Sulfoxide (DMSO) Dimethyl sulfoxide (DMSO) is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-118 25-950-CQC Size Qty/Pk Shipping Storage Expires 250 mL 1 15-30ºC 15-30ºC 12 Months 45% Glucose Solution Glucose is a main source of energy for living organisms and is used as a nutrient source in cell culture media. This 45% (w/v) solution has been ensured no cytotoxicity. Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 45001-116 25-037-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 15-30ºC 24 Months Sodium Pyruvate VWR Cat. No. Corning Cat. No. 45000-710 25-000-CI Cell Culture Reagents Sodium pyruvate is used as a component in culture broth and media as an energy source for propagating cells. It is also used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid. It is provided as a 100 mM solution with 8.5 g/L NaCl. 100 mM solution with 8.5 g/L NaCl Intended Use: In Vitro Diagnostic Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 18 Months Trace Elements Trace Elements A (1,000x) Trace Elements A provides copper, zinc, iron and selenium. Selenium aids in the detoxification of free radicals as a cofactor for GSH synthetase, while iron, copper, and zinc may be bound by serum protein. The combination of Trace Elements A contains the trace elements of MCDB 104 and of MCDB 301 Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-712 25-021-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 15-30ºC N/A 59 C E L L C U LT U R E R E A G E N T S Trace Elements Trace Elements B (1,000x) Trace Elements B solution has the same composition as Cleveland’s Trace Element I. The combination of Trace Elements B contains the trace elements of MCDB 104 and of MCDB 301 Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-714 25-022-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 2-8ºC N/A Trace Elements C (1,000x) Trace Elements C solution has the same composition of Cleveland’s Trace Elements II, and the combination of Trace Elements C contains the trace elements of MCDB 301 Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-716 25-023-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 2-8ºC N/A Tris Base Buffer, powder Cell Culture Reagents Tris is used in buffer solutions in biochemical and molecular biological research. This primary amine has an effective pH range of 7.0-9.2 and inhibits a number of different enzymes, limiting its usefulness in protein studies to some extent. This tris base buffer is provided in powder form. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 62111-160 61-233-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months 62111-158 61-233-RR 1 kg 1 15-30ºC 15-30ºC ≥ 12 Months 62111-162 61-233-RT 5 kg 1 15-30ºC 15-30ºC ≥ 12 Months Trypan Blue Trypan blue is the most common stain used in cell count and viability assays. Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue. Cells should be in suspension as single cells in buffered saline before counting. Trypan blue can be used in assays providing total cell count, viable cell count or nonviable cell count alone. This solution is provided at the common concentration of 0.4% (w/v). Trypan Blue Solution 0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4) Final pH = 7.5 ± 0.5, Osmolality (mOsm) = 305 ± 35 Intended Use: In Vitro Diagnostic 60 VWR Cat. No. Corning Cat. No. 45000-717 25-900-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 15-30ºC 24 Months Visit www.vwr.com for additional product and technical information. C E L L C U LT U R E R E A G E N T S Cell Culture Reagents 61 INSECT MEDIA AND REAGENTS Insect Media and Reagents Grace’s Insect Basal Medium Hink’s TNM-FH Medium insectagro™ Reagents insectagro™ Serum-Free Media 64 64 64 65 Insect Media & Reagents 63 INSECT MEDIA AND REAGENTS Grace’s Insect Basal Medium (Vaughn Mod.) The insect cell culture medium was formulated to closely resemble insect hemolymph, a compound crucial to the function of the insect circulatory system. The basal medium, when properly supplemented, has been used to culture cells derived from a variety of insects including several species of lepidopterans as well as some dipterans. The medium is primarily used as a basal medium for the growth and maintenance of cell lines derived from lepidopterans. Grace’s Insect Basal Medium (Vaughn Mod.), 1x With L-glutamine, without insect hemolymph Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-476 13-200-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months Hink’s TNM-FH Medium (Mod.) (Supplemented Grace’s Medium) Hink’s TNM-FH medium is a modified Grace’s formulation containing lactalbumin hydrolysate (LAH) and ultrafiltered yeastolate, and was originally developed for the optimal propagation of cabbage looper, Tricoplusi in cell. Hink’s TNM-FH Medium (Mod.), 1x With L-glutamine, lactalbumin hydrolysate, and UF Yeast Extract, without insect hemolymph Insect Media & Reagents Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-478 13-100-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months insectagro™ Reagents Poloxamer 188 Includes a non-ionic detergent that protects cells from hydrodynamic damage Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45000-488 13-901-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 2-8ºC 12 Months Poloxamer 188, Powder Includes a non-ionic detergent that protects cells from hydrodynamic damage Intended Use: Research Use Only, Not for IVD Use 64 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-486 61-161-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months Visit www.vwr.com for additional product and technical information. INSECT MEDIA AND REAGENTS insectagro™ Serum-Free Media Insect cell culture has become an increasingly important tool in biotechnology for the production of biologicals. The inherent growth characteristics of the insect cells make them ideal candidates for Baculovirus Expression Vector Systems (BEVS) for the production of recombinant genes becasue they provide advantages over both bacterial and mammalian cultures, due to their fast doubling times and ability to grow in suspension. Insect cells have significantly impacted protein production through the use of large-scale bioreactors to produce recombinant proteins and products of medical and agricultural importance with high expression levels via BEVS. Conventional insect culture media rely heavily on animal sera and other components such as yeast hydrolysate, lactalbumin hydrolysate, tryptose phosphate broth, and hemolymph as the nutritional, chemical, and physiological support systems. However, the production of purified recombinant protein has created a demand for media capable of supporting growth without relying on these additives. Serum-free growth media poses numerous advantages over the use of serum, including: Reduced foaming in most bioreactors Elimination of exogenous agents Easier downstream processing Support of high cell yields insectagro DS2™ Serum-Free/Protein-Free Medium, 1x insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and maintenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression. At the optimal temperature range 22-24°C, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension. Under these conditions, the cells require minimal adaptation to serum-free culture. Without L-glutamine Intended Use: Research Use Only, Not for IVD Use Corning Cat. No. 45000-474 13-402-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months insectagro Sf9™ Serum-Free/Protein-Free Medium insectagro Sf9™ is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. Sf9 cells are cultured in non-humidified, non-CO2 incubators at 27ºC (room temperature) and display both monolayer and suspension culture qualities. With their fast doubling times of 18-22 hours, Sf9 cells are easily scaled up to large cultures using bioreactors. With L-glutamine Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-054 13-410-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 2-8ºC 12 Months 65 Insect Media & Reagents VWR Cat. No. MICROBIOLOGY & MOLECULAR BIOLOGY Microbiology and Molecular Biology Miller’s LB Broth S.O.C Medium Terrific Broth Ampicillin Carbenicillin Kanamycin Sulfate Neomycin Sulfate Streptomycin Sulfate Tetracycline Hydrochloride X-Gal Isopropylthiogalactoside (IPTG) Phosphate Buffered Saline (PBS), 10x SSC, 20x, Liquid SSPE, 20x, Liquid TAE Buffer, 10x TBE Buffer, 10x, Liquid TE Buffer, 1x, Liquid Tris Buffered Saline, 10x, Liquid Tris-Hydrochloride Buffers, 1M EDTA, 0.5M, pH 8.0 Molecular Biology Grade Water Reagent Grade Water, 4L Cube MOPS Buffer SDS (Sodium Dodecyl Sulfate), 10% Sodium Acetate, 3M Sodium Chloride, 5M 68 68 68 68 69 69 69 69 70 70 70 71 71 71 72 72 72 73 73 74 74 74 75 75 75 75 Microbiology and Molecular Biology 67 MICROBIOLOGY & MOLECULAR BIOLOGY Microbiological Media and Components Miller’s LB Broth Miller’s LB broth is based on the original Lysogeny Broth (LB) media formulation developed by Luria and Bertani, which was designed for the rapid propagation of recombinant E. coli strains. Miller’s LB contains a 20-fold concentration sodium chloride compared to the original formulation, and is widely used for the growth and maintenance of cells in work with plasmid DNA and recombinant protein production. 10.00 g/L casein peptone, 10.00 g/L NaCl, 5.00 g/L UF yeast powder Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-048 46-050-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC N/A S.O.C Medium S.O.C medium is used as a recovery medium during the final stage of transformation of E. coli. The use of SOC medium improves transformation efficiency. 20.0 g/L casein peptone, 5.0 g/L yeast extract, 0.5845 g/L NaCl, 0.1864 g/L KCl, 0.9521 g/L MgCl2, 1.2036 g/L MgSO4, 3.6032 g/L glucose Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-099 46-003-CM Size Qty/Pk Shipping Storage Expires 10 mL 10 15-30ºC 15-30ºC 12 Months Terrific Broth Terrific Broth is a medium for the propagation and maintenance of recombinant E. coli. Because this medium supports higher cell densities than LB medium, it is preferred for maximizing plasmid yield in preparation for nucleic acid purification. 12.00 g/L casein peptone, 4.00 (mL) glycerol, 2.31 g/L KH2PO4 , 12.54 g/L K2HPO4 , 24.00 g/L UF yeast powder Microbiology and Molecular Biology Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-050 46-055-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 12 Months Ampicillin, Sodium Salt, Powder Ampicillin is a penicillin derivative that inhibits bacterial cell-wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane. A β-lactam antibiotic with an amino group side chain attached to the penicillin structure, its mode of resistance is through the cleavage of β-lactam ring of ampicillin by β-lactamase. Ampicillin is effective against Gram-positive and some Gram-negative bacteria. Intended Use: Research Use Only, Not for IVD Use 68 VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-612 61-238-RH 10 g 1 15-30ºC 2-8ºC 24-30 Months 45000-614 61-238-RM 100 g 1 15-30ºC 2-8ºC 24-30 Months Visit www.vwr.com for additional product and technical information. MICROBIOLOGY & MOLECULAR BIOLOGY Carbenicillin, Disodium Salt Carbenicillin is a form of penicillin and is often used in place of ampicillin because it maintains stability after being autoclaved. Like ampicillin, carbencillin interferes with cell-wall synthesis of actively growing cells. Molecular Weight = 422.4 Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45001-094 46-100-RG 5g 1 15-30ºC 2-8ºC 24-30 Months Kanamycin Sulfate Kanamycin is a member of the aminoglycosides a group of molecules and interacts with the 30S subunit of prokaryotic ribosomes. It induces errors in translation and thus hinders protein synthesis. The KanR-Tn5 gene product (aminoglycoside phosphotransferase) confers resistance to kanamycin. Kanamycin Sulfate is effective against Gram-negative and Gram– positive bacteria. Kanamycin Sulfate, Liquid VWR Cat. No. Corning Cat. No. 45000-810 30-006-CF Size Qty/Pk Shipping Storage Expires 50 mL 6 -15-20ºC -15-20ºC 12 Months Kanamycin Sulfate, Powder VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-640 61-176-RG 5g 1 15-30ºC 15-30ºC ≥ 12 Months Neomycin Sulfate Neomycin, a nucleoside antibiotic and aminoglycoside, is a protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3’-terminal end of aminoacyl-tRNA. It prevents growth of bacteria, protozoa, algae, and mammalian cells. Organisms carrying the resistance gene puromycin acetyltransferase are effectively protected from this compound. Neomycin is effective against Gram-negative and Gram-positive bacteria. Intended Use: Research Use Only, Not for IVD Use Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-644 61-241-RG 5g 1 15-30ºC 15-30ºC 12 Months Streptomycin Sulfate Streptomycin is an antibiotic which acts as a protein synthesis inhibitor. This aminoglycoside acts by binding to the 30S ribosomal subunit, preventing protein production and leading to bacterial cell death. Streptomyicin is more effective against Gram-positive bacteria than Gram-negative. It is offered in a powder form. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-654 61-088-RM 100 g 1 15-30ºC 2-8ºC 12 Months 69 Microbiology and Molecular Biology VWR Cat. No. MICROBIOLOGY & MOLECULAR BIOLOGY Tetracycline Hydrochloride, Powder Tetracycline Hydrochloride (HCl) is an antibiotic that inhibits protein synthesis by binding to the 30S ribosomal subunit, blocking interactions at the acceptor site on the RBA-ribosome complex. It is a member of the tetracycline group of antibiotics, a broad-spectrum class that inhibits growth of both aerobic and anaerobic organisms through this common mechanism. The congener tetracycline is semi-synthetically produced from chlortetracycline, a product of Staphylococcus aureofaciens. Resistance is conferred by the TetR-Tn10 gene product that blocks cell wall permeability. Tetracycline HCl is effective against Gram-positive and Gram-negative bacteria and is offered in powder form. Intended Use: Research Use Only, Not for IVD Use X-Gal, Powder VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45000-656 61-242-RG 5g 1 2-8ºC 2-8ºC 12 Months X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) is a substrate of b-galactosidase, a gene product of the lac operon in E. Coli. X-Gal included in bacterial medium will be hydrolyzed by ß-galactosidase to produce indoxyl, a colorimetric indicator of the activity of the lac operon. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45001-092 46-101-RF 1g 1 -15-25ºC -15-25ºC 24 Months Isopropylthiogalactoside (IPTG), Powder IPTG (isopropylthiogalactoside) is an inducer of the lac operon in bacteria, which is frequently used in cloning as a component of a recombinant plasmid. When used with X-Gal to detect lac gene activity, IPTG can be used for screening of clones containing a gene of interest in the lac operon. Intended Use: Research Use Only, Not for IVD Use Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45001-084 46-102-RF 1g 1 -15-25ºC -15-25ºC 36 Months Microbiology and Molecular Biology VWR Cat. No. 70 Visit www.vwr.com for additional product and technical information. MICROBIOLOGY & MOLECULAR BIOLOGY Buffers Phosphate Buffered Saline (PBS), 10x, pH 7.4 Phosphate buffered saline is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research. This concentrated form should be diluted to a working concentration using reagent grade water. Without calcium and magnesium pH: 7.4 ± 0.1, DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-130 46-013-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 24 Months SSC, Liquid, 20x 20x Saline Sodium Citrate (SSC), 20x, buffer is used in both nucleic acid hybridization and blot transfer procedures. 20x is the maximum concentration used; 0.2x may be used depending on the application. pH 7.0 ± 0.1, DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-046 46-020-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 24 Months SSPE, 20x, Liquid Saline Sodium Phosphate EDTA (SSPE), 20x, buffer has applications in nucleic acid hybridization and other molecular biology work. pH 7.4 ± 0.1 (20-25ºC) @ 20x, DNase, RNase, Protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-128 46-021-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 18 Months Microbiology and Molecular Biology 71 MICROBIOLOGY & MOLECULAR BIOLOGY TAE Buffer, 10x Tris Acetate EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer. Linear, double-stranded DNA separates faster in a TAE buffer-based system compared to Tris Borate EDTA (TBE) buffer, but the latter has a higher buffering capacity and is thus more resistant to changes in pH. Use of TAE vs TBE buffer depends on downstream applications. TAE is recommended for preparative electrophoresis in which the nucleic acid bands being separated are to be used in cloning, and other work requiring enzymatic applications. pH 8.3 ± 0.1 (24-26ºC), DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-074 46-010-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 24 Months TBE Buffer, 10x, Liquid TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis. TBE has a higher buffering capacity than Tris-AcetateEDTA (TAE) buffer, but the latter is more suitable for steps involving the action of enzymes on nucleic acids because borate is an enzymatic inhibitor. Electrophoresis using TBE-containing agarose gels and TBE running buffer is much more suitable for size analysis of the nucleic acids being separated, as TBE buffer allows these nucleic acids to better maintain their structural integrity. pH: 8.4 ± 0.1 (24-26ºC), DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-072 46-011-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 24 Months TE Buffer, 1x, Liquid Tris-EDTA (TE) buffer is commonly used in work with nucleic acids. It can be included as a solubilization buffer in DNA and RNA purification, as a running buffer in electrophoresis, and as a general storage buffer for nucleic acids. pH: 7.9-8.1 (20-25ºC), DNase, RNase, protease tested Microbiology and Molecular Biology Intended Use: Research Use Only, Not for IVD Use 72 VWR Cat. No. Corning Cat. No. 45001-154 46-009-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 12 Months Visit www.vwr.com for additional product and technical information. MICROBIOLOGY & MOLECULAR BIOLOGY Tris Buffered Saline, 10x, Liquid Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively dramatically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature. pH: 7.4 ± 0.1 (24-26ºC) @ 1x, DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-132 46-012-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 12 Months Tris-Hydrochloride Buffers, 1m Tris-HCI buffer has many applications in molecular biology work, including use in nucleic acid purification. This product can be diluted to obtain the desired concentration. 1M Tris-HCl, pH 7.5, Liquid pH: 7.5 ± 0.1 (24-26ºC), DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-066 46-030-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 24 Months 1M Tris-HCl, pH 8.0, Liquid pH: 8.0 ± 1.0 (24-26ºC), DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-064 46-031-CM Size Qty/Pk Shipping Storage Expires 1L 6 15-30ºC 15-30ºC 24 Months Microbiology and Molecular Biology 73 MICROBIOLOGY & MOLECULAR BIOLOGY Reagents EDTA, 0.5m, pH 8.0 EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to “sequester” metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution, but exhibit diminished reactivity. The use of EDTA allows for greater control over many experiments. 0.5 EDTA, pH 8.0 Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-122 46-034-CI Size Qty/Pk Shipping Storage Expires 100 mL 6 15-30ºC 15-30ºC 24 Months Molecular Biology Grade Water Molecular biology grade water is ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where Rnase, Dnase, and Protease-free water is required. No toxic agents, including DEPC, are used in the preparation of our molecular biology grade water, eliminating possible interferences with enzymatic reactions. pH 6.0 ± 1.0, DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 95000-094 Size Qty/Pk Shipping Storage Expires 46-000-CI 100 mL 6 15-30ºC 15-30ºC 24 Months 91002-726 46-000-CV 500 mL 6 15-30ºC 15-30ºC 24 Months 45001-044 46-000-CM 1L 6 15-30ºC 15-30ºC 24 Months Reagent Grade Water, 4L Cube Microbiology and Molecular Biology Reagent grade water is ideal for the preparation of reagents, rinsing glassware and plastic ware, cleaning electrodes and other common laboratory tasks requiring highly pure water. No toxic agents, including DEPC, are used in its preparation. The packaging design allows for portability, ease of storage and convenient dispensing. Upon packaging, the water meets USP conductivity requirements for water for injection (WFI) and is RNAse, DNAse and protease free. 74 VWR Cat. No. Corning Cat. No. 97060-878 46-002-LF Size Qty/Pk Shipping Storage Expires 4L 2 15-30ºC 15-30ºC N/A Visit www.vwr.com for additional product and technical information. MICROBIOLOGY & MOLECULAR BIOLOGY MOPS Buffer MOPS is a zwitterionic buffer used in denaturing agarose gel electrophoresis of RNA and in SDS-PAGE protein analysis. It has a pH range of 6.5 - 7.9. Molecular Weight = 209.27 Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 45001-134 46-103-RM 100 g 1 15-30ºC 15-30ºC N/A SDS (Sodium Dodecyl Sulfate), 10% Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2 µm filtered. Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-120 46-040-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 15-30ºC 24 Months Sodium Acetate, 3m Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution. pH 5.2 ± 0.1 (20-25ºC), DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-124 46-033-CI Size Qty/Pk Shipping Storage Expires 100 mL 1 15-30ºC 15-30ºC 24 Months Sodium Chloride, 5m Microbiology and Molecular Biology 5M Sodium Chloride, (NaCl) is a common molecular biology reagent. It is tested to ensure that they are free of RNAse. This product is used to control osmolality and conductivity, among other factors. DNase, RNase, protease tested Intended Use: Research Use Only, Not for IVD Use VWR Cat. No. Corning Cat. No. 45001-125 46-032-CV Size Qty/Pk Shipping Storage Expires 500 mL 6 15-30ºC 15-30ºC 24 Months 75 H I G H Q UA L I T Y WAT E R High Quality Water Distilled, Deionized Water USP/EP Certified Sterile WFI Quality Water 79 79 High Quality Water 77 High Quality Water H I G H Q UA L I T Y WAT E R 78 Visit www.vwr.com for additional product and technical information. H I G H Q UA L I T Y WAT E R Sterile WFI-Quality, Cell Culture Grade Water Corning cellgro’s state-of-the-art in-house water system provides purified water, free of any added components, ideal for many applications in the research and biopharmaceutical industry. This advanced process incorporates innovative techniques to reduce bioburden and maintain low endotoxin levels. Applications include: Raw material for manufacturing, cell culture, process validation, device processing, purification buffers, and chromatography buffers. Distilled, Deionized Water Meets WFI quality standards Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. 95000-092 Size Qty/Pk Shipping Storage Expires 25-055-CI 100 mL 6 15-30ºC 15-30ºC 36 Months 45000-672 25-055-CV 500 mL 6 15-30ºC 15-30ºC 36 Months 45001-042 25-055-CM 1L 6 15-30ºC 15-30ºC 36 Months 45000-674 25-055-LB 20L 1 15-30ºC 15-30ºC 36 Months 45001-270 25-055-LG 100L 1 15-30ºC 15-30ºC 36 Months 45001-088 25-055-LH 200L 1 15-30ºC 15-30ºC 36 Months USP/EP Certified Sterile WFI-Quality Water USP/EP Certified Water is validated to the most recent specifications found in the United States Pharmacopeia (USP) and the European Pharmacopeia (EP). The specific testing is in accordance to testing for “Sterilized Water for Injection”, (European Pharmacopeia), and “Sterile Water for Injection”, (United States Pharmacopeia). This product is intended for Further Manufacturing Use Only in a multitude of applications, from used with Steam-in-Place (SIP) systems and equipment to Clean-in-Place (CIP) systems and synthesis of a final product. This water is also suitable for product reconstitution and for use as a cleaning agent for rinsing vessels and equipment. USP/EP Certified Sterile WFI-Quality Water USP/EP Certified Intended Use: In Vitro Diagnostic VWR Cat. No. Corning Cat. No. Size Qty/Pk Shipping Storage Expires 95029-816 25-065-LB 20L 1 15-30ºC 15-30ºC 36 Months 95029-818 25-065-LG 100L 1 15-30ºC 15-30ºC 36 Months 95029-820 25-065-LHÍ 200L 1 15-30ºC 15-30ºC 36 Months High Quality Water 79 Alphabetic Index A H Amino Acids and Vitamins Amphotericin B Ampicillin Ampicillin, Sodium Salt Antibiotic-Antimycotic Solution Antibiotics and Antimycotics 51 52 52 68 53 52 B Blasticidin S HCl Buffered Salt Solutions Buffers 53 24 56, 71 C Carbenicillin, Disodium Salt Cell Culture Reagents cellgro COMPLETE™ Cellstripper™ Charcoal-Stripped FBS Chloramphenicol Ciprofloxacin Hydrochloride Classical Media CMRL 1066 69 50 32 58 46 53 53 6 7 D Dialyzed FBS Dimethyl Sulfoxide DMEM DMEM/Ham’s F-12 50/50 Mix Donor Calf Serum Donor Horse Serum Dulbecco’s Phosphate Buffered Saline 46 60 7 11 44 45 25 E EDTA, 0.5M, pH 8.0 ES Cell Tested FBS 74 46 F F-12K Nutrient Mixture Fetal Bovine Serum 12 43 G G418 Sulfate Gamma-Irradiated FBS Gentamicin Sulfate Glasgow’s MEM Glucose Solution glutagro™ Grace’s Insect Basal Medium Growth Supplements 53 47 54 12 60 51 64 58 Ham’s F-10 Medium Ham’s F-12 Medium Hanks’ Balanced Salt Solution HAT HEPES Hink’s TNM-FH Medium Human AB Serum Hybridoma Reagents Hygromycin B Solution P 13 13 26 58 56 64 45 58 54 I Improved MEM (Richter’s Mod.) insectagro DS2™ insectagro Sf9™ insectagro™ Reagents insectagro™ Serum-Free Media Insect Media and Reagents Insulin-Transferrin-Selenium Iscove’s Modification of DMEM Isopropylthiogalactoside 14 33, 65 33, 66 64 65 63 58 14 70 K Kanamycin Sulfate 54, 71 L Leibovitz’s L-15 L-Glutamine Low IgG FBS Lymphocyte Separation Medium 15 51 47 34 M McCoy’s 5A MCDB 131 Medium 199 MEM Amino Acids MEM Vitamins Microbiology and Molecular Biology Miller’s LB Broth Minimum Essential Medium Miscellaneous Reagents Molecular Biology Grade Water MOPS Buffer 15 15 16 52 52 69 70 17 59 76 75 N Neomycin Sulfate 55, 71 Non-Enzymatic Cell Dissociation Agents60 Penicillin Streptomycin Solution Phosphate Buffered Saline Phosphate Buffered Saline, pH 7.4 Polysucrose 400 Porcine Serum Puromycin Dihydrochloride 55 26 71 34 45 55 R Reagent Grade Water rhAlbumin rhLactoferrin RPMI 1640 74 35 36 20 S Sera 40 Serum-Free and Speciality Media 32 S.O.C Medium 68 75 Sodium Acetate 3M Sodium Bicarbonate 56 Sodium Chloride 5M 75 Sodium Dodecyl Sulfate 10% 75 Sodium Pyruvate 60 Specialized Serum 46 SSC, 20x 71 SSPE, 20x 71 stemgro® AB2™ Neural Base Media 36 stemgro® ABS™ Neural Supplement 36 Streptomycin Sulfate 55, 69 T TAE, 10x TBE Buffer, 10x TE Buffer, 1x Terrific Broth Tetracycline Hydrochloride Tetracycline Negative FBS Trace Elements transfectagro™ Tris Base Buffer Tris Buffered Saline, 10x Tris-Hydrochloride Buffers, 1m Trypan Blue Trypsin Tryptic Soy Broth 72 72 72 68 55, 70 47 60 21 61 73 73 61 57 35 W Water 79 X X-Gal 70 Numeric Index Corning cellgro Category Number 10-010 10-013 10-014 10-016 10-017 10-022 10-024 10-025 10-026 10-040 10-041 10-043 10-045 10-050 10-051 10-060 10-070 10-080 10-090 10-092 10-101 10-102 10-104 13-100 13-200 13-402 13-410 13-415 13-901 15-010 15-012 15-013 15-015 15-016 15-017 15-018 15-040 15-041 15-090 15-100 15-110 16-405 17-104 17-105 17-204 17-205 17-206 17-305 20-021 20-023 20-030 20-031 21-020 21-021 21-022 21-023 21-030 21-031 18 8 7 14 8 17 14 12 14 20 21 22 15 16 16 16 13 15 13 13 9 9 20 64 64 33, 65 33, 66 33, 66 64 18 17 8 18 14 8 9 20 21 11 15 7 11 21 20 9 9 9 18 26 26, 32 25 25 27 27 27 27 25 25 21-040 25-000 25-005 25-015 25-021 25-022 25-023 25-025 25-030 25-035 25-037 25-040 25-046 25-047 25-050 25-051 25-052 25-053 25-054 25-055 25-056 25-060 25-065 25-072 25-800 25-900 25-950 30-001 30-002 30-003 30-004 30-005 30-006 30-009 30-234 30-240 35-010 35-011 35-015 35-016 35-022 35-030 35-040 35-060 35-070 35-071 35-072 35-073 35-074 35-075 40-101 40-102 40-200 40-300 40-400 46-000 46-002 46-003 26 60 51 51, 52 59 60 60 52, 53 52 56 60 36 58 58 57 57 57 57 57 59 58 56 59 34 58 61 60 55 55 52 53 54 54, 69 55 53 54 44 44 43 43 44 45 45 45 43, 46, 47 43, 46 43, 46 46, 47 44 44, 47 32 32 32, 34 21 36 74 74 68, 75 46-009 46-010 46-011 46-012 46-013 46-020 46-021 46-030 46-031 46-032 46-034 46-040 46-050 46-055 46-060 46-100 46-101 46-102 46-103 50-003 50-004 50-010 50-011 50-012 50-013 50-020 50-040 50-045 50-050 50-051 51-010 55-021 55-022 55-031 61-030 61-034 61-065 61-088 61-098 61-161 61-176 61-233 61-234 61-238 61-239 61-241 61-242 61-277 61-385 61-411 62-450 62-451 90-009 90-013 90-050 90-090 90-091 90-113 74 72 72 73 26, 71 71 71 73 73 75 74 75 68 68 35 69 70 70 75 10 12 19 19 17 10 21 13 15 16 17 19 27 27 25 51 56 56 55, 69 54 64 54, 69 61 54 52, 68 53 55, 69 55, 70 53 55 35 35 36 19 10 17 12 12 10 Numeric Index VWR Category Number 45000-300 45000-302 45000-304 45000-306 45000-308 45000-310 45000-312 45000-314 45000-316 45000-318 45000-320 45000-322 45000-324 45000-326 45000-328 45000-330 45000-332 45000-334 45000-336 45000-340 45000-342 45000-344 45000-346 45000-348 45000-350 45000-352 45000-354 45000-356 45000-358 45000-360 45000-362 45000-364 45000-366 45000-368 45000-370 45000-372 45000-374 45000-376 45000-378 45000-380 45000-382 45000-384 45000-386 45000-388 45000-394 45000-396 45000-398 45000-400 45000-402 45000-404 45000-406 45000-408 45000-410 45000-412 45000-414 45000-416 45000-418 45000-420 45000-422 45000-424 45000-426 17 17 8 8 8 8 8 8 8 8 8 8 8 8 9 9 8 8 9 9 9 11 11 11 11 11 12 13 13 13 14 14 14 14 14 15 16 16 16 18 18 18 18 18 18 20 20 20 20 20 20 20 20 21 21 21 21 32 32 32 25 45000-428 45000-430 45000-432 45000-434 45000-436 45000-438 45000-440 45000-442 45000-444 45000-446 45000-448 45000-450 45000-452 45000-454 45000-456 45000-458 45000-460 45000-462 45000-464 45000-466 45000-470 45000-474 45000-476 45000-478 45000-486 45000-488 45000-504 45000-518 45000-520 45000-522 45000-524 45000-526 45000-534 45000-536 45000-540 45000-544 45000-550 45000-562 45000-570 45000-574 45000-578 45000-582 45000-584 45000-586 45000-592 45000-594 45000-596 45000-602 45000-604 45000-612 45000-614 45000-616 45000-618 45000-620 45000-622 45000-624 45000-626 45000-628 45000-630 45000-632 45000-634 25 25 25 25 25 25 25 25 25 26 26 26 27 27 27 27 27 27 27 27 27 33, 65 64 64 64 64 17 10 10 10 10 10 10 12 12 12 13 15 16 17 17 19 19 19 19 19 19 21 21 52, 68 52 53 53 53 53 54 54 54 53 54 54 45000-636 45000-640 45000-644 45000-650 45000-652 45000-654 45000-656 45000-658 45000-664 45000-666 45000-668 45000-672 45000-674 45000-676 45000-678 45000-680 45000-682 45000-684 45000-686 45000-688 45000-690 45000-692 45000-694 45000-696 45000-698 45000-700 45000-702 45000-704 45000-706 45000-708 45000-710 45000-712 45000-714 45000-716 45000-717 45000-726 45000-728 45000-730 45000-732 45000-734 45000-736 45000-806 45000-808 45000-810 45001-042 45001-044 45001-046 45001-048 45001-050 45001-052 45001-054 45001-058 45001-060 45001-062 45001-064 45001-066 45001-072 45001-074 45001-076 45001-082 45001-084 54 54, 69 55, 69 55 55 55,69 55, 70 57 57 57 58 79 79 51 51 51 51 51 58 58 56 56 56 56 52 52 52 56 56 56 59 59 60 60 60 34 34 34 34 44 44 54 52 54, 69 79 74 71 68 68 11 33, 65 45 44 45 73 73 72 72 53 57 70 45001-086 45001-088 45001-090 45001-092 45001-094 45001-099 45001-101 45001-103 45001-106 45001-108 45001-110 45001-112 45001-114 45001-116 45001-118 45001-120 45001-122 45001-124 45001-125 45001-128 45001-130 45001-132 45001-134 45001-152 45001-154 45001-156 45001-226 45001-270 45001-660 45001-662 62111-158 62111-160 62111-162 62111-170 71002-674 71002-676 71002-816 71002-872 71002-874 71002-876 71002-878 89133-078 89133-080 91002-726 95000-092 95000-094 95029-816 95029-818 95029-820 97060-260 97060-262 97060-876 97060-878 97065-300 97065-302 97065-304 97065-306 97065-308 97065-310 51 79 58 70 69 68 27 55 43 43 21 15 7 59 59 75 74 75 75 71 26, 71 73 75 26 72 35 35 79 57 57 60 60 60 55 11 53 33 9 9 12 20 35 36 74 79 74 79 79 79 36 36 9 74 43, 46, 47 43, 46 43, 46 44, 47 44 44, 47 ® cellgro® ® ® cellgro products are manufactured by Mediatech, Inc. For a listing of Corning trademarks, visit us at www.cellgro.com/about-us/trademarks.html All other trademarks in this document are the property of their respective owners. Corning Incorporated, One Riverfront Plaza, Corning, NY 14831-0001 1012 7M Lit. No. 03090 © 2012 Corning Incorporated Printed in U.S.A. 10/12 POD CLS-CG-BR-002 VWR The Corning Family of Brands
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