Download Powerpoint - Blood Journal

Symmetry of Initial Cell Divisions Among Primitive
Hematopoietic Progenitors Is Independent of Ontogenic
Age and Regulatory Molecules
by Shiang Huang, Ping Law, Karl Francis, Bernhard O. Palsson, and Anthony D. Ho
Blood
Volume 94(8):2595-2604
October 15, 1999
©1999 by American Society of Hematology
This figure demonstrates a typical symmetric division of one CD34+/CD38− cell derived from
FLV. We found that the first cell division occurred consistently at 36 to 38 hours after culturing.
Shiang Huang et al. Blood 1999;94:2595-2604
©1999 by American Society of Hematology
Divisional history of 1 single CD34+/CD38− cell stained with PKH26, derived from an FLV
sample.
Shiang Huang et al. Blood 1999;94:2595-2604
©1999 by American Society of Hematology
Other CD34+/CD38−cells initially gave rise to 2 daughter cells that appeared equivalent, but then
divided asymmetrically after the second or third division.
Shiang Huang et al. Blood 1999;94:2595-2604
©1999 by American Society of Hematology
Percentages of asymmetric divisions found after the first, second, third, and the fifth cell
division.
Shiang Huang et al. Blood 1999;94:2595-2604
©1999 by American Society of Hematology
Correlation between replication behavior of the CD34+/CD38− cells in the first 4 days with the
growth pattern of the corresponding single cell after 14 days of culture.
Shiang Huang et al. Blood 1999;94:2595-2604
©1999 by American Society of Hematology
Mitotic rates, asymmetric divisions, colony efficiencies, and ADI of CD34+/CD38− candidate
hematopoietic stem cells upon exposure to regulatory molecules: recombinant human TPO,
FL3-L, and SCF. Cytokine cocktail (containing Epo, IL-3, IL-6, GM-CSF, SCF, bFG...
Shiang Huang et al. Blood 1999;94:2595-2604
©1999 by American Society of Hematology