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Endothelial progenitor cell–derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA by Maria Chiara Deregibus, Vincenzo Cantaluppi, Raffaele Calogero, Marco Lo Iacono, Ciro Tetta, Luigi Biancone, Stefania Bruno, Benedetta Bussolati, and Giovanni Camussi Blood Volume 110(7):2440-2448 October 1, 2007 ©2007 by American Society of Hematology Characterization of EPC-derived MVs. (A) Representative FACS analysis of MVs (dark) and of 1-, 2-, 4-, and 6-μm beads used as internal size standards. Maria Chiara Deregibus et al. Blood 2007;110:2440-2448 ©2007 by American Society of Hematology Incorporation of EPC-derived MVs in HMECs. (A) Representative FACS analysis of internalization, after a 30-minute incubation at 37°C, by HMECs of MVs labeled with PKH26 (filled curve). Maria Chiara Deregibus et al. Blood 2007;110:2440-2448 ©2007 by American Society of Hematology Proliferative and antiapoptotic effects of EPC-derived MVs. (A,B) Eight thousand cells/well (A, HMECs; B, HUVECs) into 96-well plates were added with 10 μM BrdU, incubated for 72 hours in EBM-2 without FCS and growth factors in the presence of vehicle alone... Maria Chiara Deregibus et al. Blood 2007;110:2440-2448 ©2007 by American Society of Hematology In vitro proangiogenic effects of EPC-derived MVs. (A) HMECs (■) or HUVECs (□) (5 × 104 cells/well) were plated on growth factor–reduced Matrigel in DMEM + 0.25% BSA and were challenged for 6 hours at 37°C with vehicle alone (M199 medium) as control or with... Maria Chiara Deregibus et al. Blood 2007;110:2440-2448 ©2007 by American Society of Hematology In vivo proangiogenic effects of EPC-derived MV. Formation of vessels after 7 days by HMECs preincubated (30 minutes at 37°C) with 30 μg/mL of MVs and then injected subcutaneously in Matrigel in SCID mice. Maria Chiara Deregibus et al. Blood 2007;110:2440-2448 ©2007 by American Society of Hematology mRNA transfer by EPC-derived MVs. HMECs (5 × 104 cells/well) were incubated for 24 hours in DMEM plus 5% BSA with 10 μg/mL MVs derived from GFP-EPC, and the synthesis of GFP protein was evaluated by confocal microscopy (Zeiss LSM 5 Pascal confocal Laser sca... Maria Chiara Deregibus et al. Blood 2007;110:2440-2448 ©2007 by American Society of Hematology MVs induced Akt activation and expression of Bcl-xL and eNOS. Maria Chiara Deregibus et al. Blood 2007;110:2440-2448 ©2007 by American Society of Hematology
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