Download Figure S1.

C
7--hydroxysteroid
dehydrogenase
Glycosyl hydrolase
CotH protein
tubulin/FtZ, GTPase
VTC domain superfamily
resolvase
tubulin/FtZ, GTPase
VTC domain superfamily
B
Extracelluar soulute
binding protein
Resolvase
Sigma factor SgiI
Β lac domain
MarR regulator
MGA regulator
SAM-domain
Internalin
netB
ricin-domain
conttaining protein
transposase
Chitinase B
Chitinase A
Leukocidin I
Leukocidin II
CAAX protease
recombinase
membrane protein
membrane protein
F5/8 type C domain
phosphodiesterase
sortase
surface protein
peptidoglycan bound
cell-wall protein
swim zinc finger
ABC transporter
Signal peptidase I
diguanylate cyclase
Genetic organization of NE loci
A
42 kb
11.2 kb
Putative role:
Toxin
Adhesion
Regulation
Mobilization
Enzyme
Other
Plasmid
Hypothetical protein
5.6 kb
Lepp D, Roxas B, Parreira VR, Marri PR, Rosey EL, et al. (2010) Identification of novel pathogenicity loci in Clostridium
perfringens strains that cause avian necrotic enteritis. PLoS One 5:e10795
Figure S1 (A). Overlapping PCR analysis of NE locus 1
2
(4.5Kb)
3
(2.7Kb)
4
(4.9Kb)
5
(5.5Kb)
6
(3Kb)
7
(3.6Kb)
8
(2.2Kb)
9
(3.9Kb)
10
(3.1Kb)
11
(2.8K
b)
12
(4.1Kb)
13
(2.9Kb)
NE04
+
+
+
+
+
+
+
+
+
+
+
+
+
NE09
+
+
+
+
+
+
+
+
+
+
+
+
+
NE10
+
+
+
+
+
+
+
+
+
+
+
+
+
NE14
+
+
+
+
+
+
+
+
+
+
+
+
+
NE20
+
+
+
+*
+
+
+
+
+
+
+
+
+
NE23
+
+
+
+
+
+
+
+
+
+
+
+
+
NE28
+
+
+
+
+
+
+
+
+
+
+
+
+
NE30
+
+
+
+
+
+
+
+
+
+
+
+
+
+
5
+
+
+
+
+
+
+
+
+
+
+
+
4
+
+
+
+
+
+
+
+
+
+
+
+
4
+
+
+
+
+
+
+
+
+
+
+
NE42
H26
H34
+, PCR product was of expected size ; -, no PCR product produced, if the PCR product did not match the expected size, the actual size is given; * two bands
lexA
Β lac domain
MarR regulator
MGA regulator
SAM-domain
Internalin
netB
ricin-domain
transposase
Chitinase B
Chitinase A
CAAX protease
Leukocidin II
Leukocidin I
recombinase
membrane protein
membrane protein
F5/8 type C domain
phosphodieserasediguanylate cyclase
sortase
surface protein
cell-wall protein
peptidoglycan bound
Signal peptidase I
ABC transporter
swim zinc finger
diguanylate cyclase
dcm
1
(4.1Kb)
Glycosyl hydrolase
CotH protein
tubulin/FtZ, GTPase
VTC domain superfamily
resolvase
Sigma factor SgiI
tubulin/FtZ, GTPase
Table S1 (B). Overlapping PCR analysis of NE locus 2
1
(0.9Kb)
2
(2.3Kb)
3
(3.0Kb)
4
(4.0Kb)
5
(1.9Kb)
NE04
+
+
+
+
+
NE09
+
+
+
+
+
NE10
+
+
+
+
+
NE14
+
+
+
+
+
NE20
+
+
+
+
+
NE23
+
+
+
+
+
NE28
+
+
+
+
+
NE30
+
+
+
+
-
NE42
+
+
+
+
+
H26
0.7
+
+
+
+
H34
0.7
+
+
+
+
+, PCR product was of expected size ; -, no PCR product produced, if the PCR product did not match the expected size, the actual size is given
pCP8533etx_p34
7--hydroxysteroid
dehydrogenase
Extracelluar soulute
binding protein
Resolvase
pCP8533etx_p25
Table S1 (C). Overlapping PCR analysis of NE locus 3
1
(2.9Kb)
2
(0.9Kb)
3
(2.9Kb)
4
(1.9Kb)
NE04
+
+
+
+
NE09
+
+
+
+
NE10
+
+
+
+
NE14
+
+
+
+
NE20
+
+
+
+
NE23
+
+
+
+
NE28
-
-
-
+
NE30
-
-
-
+
NE42
-
+
+
-
H26
-
-
-
+
H34
+
+
+
+
+, PCR product was of expected size ; -, no PCR product produced, if the PCR product did not match the expected size, the actual size is given