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C 7--hydroxysteroid dehydrogenase Glycosyl hydrolase CotH protein tubulin/FtZ, GTPase VTC domain superfamily resolvase tubulin/FtZ, GTPase VTC domain superfamily B Extracelluar soulute binding protein Resolvase Sigma factor SgiI Β lac domain MarR regulator MGA regulator SAM-domain Internalin netB ricin-domain conttaining protein transposase Chitinase B Chitinase A Leukocidin I Leukocidin II CAAX protease recombinase membrane protein membrane protein F5/8 type C domain phosphodiesterase sortase surface protein peptidoglycan bound cell-wall protein swim zinc finger ABC transporter Signal peptidase I diguanylate cyclase Genetic organization of NE loci A 42 kb 11.2 kb Putative role: Toxin Adhesion Regulation Mobilization Enzyme Other Plasmid Hypothetical protein 5.6 kb Lepp D, Roxas B, Parreira VR, Marri PR, Rosey EL, et al. (2010) Identification of novel pathogenicity loci in Clostridium perfringens strains that cause avian necrotic enteritis. PLoS One 5:e10795 Figure S1 (A). Overlapping PCR analysis of NE locus 1 2 (4.5Kb) 3 (2.7Kb) 4 (4.9Kb) 5 (5.5Kb) 6 (3Kb) 7 (3.6Kb) 8 (2.2Kb) 9 (3.9Kb) 10 (3.1Kb) 11 (2.8K b) 12 (4.1Kb) 13 (2.9Kb) NE04 + + + + + + + + + + + + + NE09 + + + + + + + + + + + + + NE10 + + + + + + + + + + + + + NE14 + + + + + + + + + + + + + NE20 + + + +* + + + + + + + + + NE23 + + + + + + + + + + + + + NE28 + + + + + + + + + + + + + NE30 + + + + + + + + + + + + + + 5 + + + + + + + + + + + + 4 + + + + + + + + + + + + 4 + + + + + + + + + + + NE42 H26 H34 +, PCR product was of expected size ; -, no PCR product produced, if the PCR product did not match the expected size, the actual size is given; * two bands lexA Β lac domain MarR regulator MGA regulator SAM-domain Internalin netB ricin-domain transposase Chitinase B Chitinase A CAAX protease Leukocidin II Leukocidin I recombinase membrane protein membrane protein F5/8 type C domain phosphodieserasediguanylate cyclase sortase surface protein cell-wall protein peptidoglycan bound Signal peptidase I ABC transporter swim zinc finger diguanylate cyclase dcm 1 (4.1Kb) Glycosyl hydrolase CotH protein tubulin/FtZ, GTPase VTC domain superfamily resolvase Sigma factor SgiI tubulin/FtZ, GTPase Table S1 (B). Overlapping PCR analysis of NE locus 2 1 (0.9Kb) 2 (2.3Kb) 3 (3.0Kb) 4 (4.0Kb) 5 (1.9Kb) NE04 + + + + + NE09 + + + + + NE10 + + + + + NE14 + + + + + NE20 + + + + + NE23 + + + + + NE28 + + + + + NE30 + + + + - NE42 + + + + + H26 0.7 + + + + H34 0.7 + + + + +, PCR product was of expected size ; -, no PCR product produced, if the PCR product did not match the expected size, the actual size is given pCP8533etx_p34 7--hydroxysteroid dehydrogenase Extracelluar soulute binding protein Resolvase pCP8533etx_p25 Table S1 (C). Overlapping PCR analysis of NE locus 3 1 (2.9Kb) 2 (0.9Kb) 3 (2.9Kb) 4 (1.9Kb) NE04 + + + + NE09 + + + + NE10 + + + + NE14 + + + + NE20 + + + + NE23 + + + + NE28 - - - + NE30 - - - + NE42 - + + - H26 - - - + H34 + + + + +, PCR product was of expected size ; -, no PCR product produced, if the PCR product did not match the expected size, the actual size is given