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Short survival of phosphatidylserine-exposing red blood cells in murine sickle cell anemia by Kitty de Jong, Renee K. Emerson, James Butler, Jacob Bastacky, Narla Mohandas, and Frans A. Kuypers Blood Volume 98(5):1577-1584 September 1, 2001 ©2001 by American Society of Hematology Morphology of murine sickle cells mimics human sickle cells.Human (left) and murine (right) RBCs were fixed under oxygenating (top) or during deoxygenating conditions (bottom), as described in “Materials and methods.” After processing, the cells were examin... Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology Cell deformability is altered in murine sickle cells.Osmotic deformability profiles of 4 murine sickle (SS) and wild-type RBCs (WT) (A) and 2 patients with sickle cell anemia (SS) and the range of normal RBCs (AA) (B). Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology MCV and MCH.Volume (A) and hemoglobin concentration (B) histograms of RBCs and reticulocytes. Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology Reduced RBC survival in murine sickle cell anemia and thalassemia.At t = 0, murine RBCs were biotinylated by tail vein injection of sulfo-NHS-biotin. Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology Morphologic comparison of biotinylated cells.Corresponding visual fields from bright-field (A) and fluorescent (B) micrographs of Oregon green streptavidin–labeled RBCs recorded at 3 days after biotinylation. Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology Irreversible sickle cell formation during the lifespan of the murine sickle cell.Percentage of fluorescent cells in the population as measured by fluorescence microscopy (□, solid line), total percentage of ISCs in the population as counted by microscopy (●... Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology PS is exposed on a subpopulation of murine sickle cells.Murine RBCs were labeled with fluorescent annexin V and analyzed by flow cytometry. Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology Reduced RBC survival time of PS-exposing sickle cells.Murine RBC population was biotinylated on day 0, and at the indicated time intervals, RBCs were sampled and double-labeled with PEstreptavidin and GFP–annexin V to assess the survival of PS-exposing cel... Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology PS exposure in murine sickle cell bone marrow.RBCs (A) were labeled with biotin–annexin V– PE-streptavidin and thiazole orange. Kitty de Jong et al. Blood 2001;98:1577-1584 ©2001 by American Society of Hematology
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