Download 3D Bioprinting For Cartilage

3D Bioprinting For Cartilage
Lillian Margolis
Biomedical Engineering
October 21, 2015
Introduction
• Tissue Engineering
– Study of growth of
connective tissue
– Repair or replace tissue
• Cartilage
– Connective tissue
– Avascular
– Three Types: Hyaline,
Elastic, and Fibrous
• 3D Bioprinting: scaffolds and
bio-ink
[1]
[2]
Methods
• Scaffolds: three
dimensional polymer
mold that guides the
tissue as it cultures and
grows
– Mesenchymal stem cells
– Chemical cues to mimic
the original tissue
[3]
[3]
Methods
• 3D Bioprinting: directly repair or recreate
cartilage and integrates with original cartilage
– Sizes of tissues printed: under 400 micrometers
– 5 options
• Extrusion
• Laser
• Inkjet
• Thermal Inkjet
• Piezoelectric Inkjet
[4]
[5]
Studies
• Thermal Inkjet Study (Human)
– Layer by layer, articular cartilage, and polyethylene glycol
dimethacrylate
– 4mm diameter, thickness of 2mm, nominal 0.23 microliters bioink
– ~1140 chondrocytes
– Each layer printed and photopolymerized, 18micrometers thick
– 2 mins total printing time
– Printed cartilage with 3d biopaper had higher levels of
glycosaminoglycan (GAG) content than cartilage printed without
– Result: Importance of direct cartilage repair; success in
placement of individual cells, preserving cell viability, maintaining
chondrogenic phenotype, and integrating with original tissue
tissue
[5]
Studies
• 4 Bioinks: Ink9010, Ink8020, Ink7030, and Ink6040
• Printed small grids with the four different bioinks and
crossed-linked them with CaCl2 for 10 mins
• Compression testing and shape fidelity testing
• The different ink compositions can be used for different
printing depending on mechanical properties
[6]
[6]
[6]
Conclusion
• Thermal Inkjet Bioprinting
– Print both soft and hard tissue
– Best option for repairing cartilage
• Ink8020 is most suitable bioink for printing
• Future
– Optimizing scaffolds
– Targeted Drug Therapy
– Gene Transfection
[YW]
Questions?
[1]
Resources Besides the Ones in the Abstract
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7.
(n.d.). Retrieved October 17, 2015, from
http://www.millerplace.k12.ny.us/webpages/lmiller/photos/636532/large23_Cartilage Types.jpg
W HAT IS TISSUE ENGINEERING. (N.D.). RETRIEVED OCTOBER 17, 2015, FROM HTTP://WWW.RPI.EDU/DEPT/CHEMENG/BIOTECH-ENVIRON/PROJECTS00/TISSUE/W HAT IS TISSUE ENGINEERING.HTM
Camarero-Espinosa, S., Rothen-Rutishauser, B., Weder, C., & Foster, E. (2015). Directed cell growth in multi-zonal
scaffolds for cartilage tissue engineering. Biomaterials, 42-52. doi:10.1016/j.biomaterials.2015.09.033
Murphy, S., & Atala, A. (2014). 3D bioprinting of tissues and organs. Nat Biotechnol Nature Biotechnology, 773-785.
doi:10.1038/nbt.2958
Gao, G., & Cui, X. (2015). Three-dimensional bioprinting in tissue engineering and regenerative medicine.
Biotechnology Letters, 1-9. doi:10.1007/s10529-015-1975-1
Markstedt, K., Mantas, A., Tournier, I., Ávila, H., Hägg, D., & Gatenholm, P. (2015). 3D Bioprinting Human
Chondrocytes with Nanocellulose–Alginate Bioink for Cartilage Tissue Engineering Applications. BioMacroMolecules,
1489-1496. doi:10.1021/acs.biomac.5b00188
Digital image. University of Rhode Island. N.p., n.d. Web. <http://www.uri.edu/news/releases/html/images/rhody.jpg>.