Download Powerpoint - Blood Journal

Impaired “outside-in” integrin αIIbβ3 signaling and
thrombus stability in TSSC6-deficient mice
by Matt W. Goschnick, Lai-Man Lau, Janet L. Wee, Yong S. Liu, P. Mark Hogarth,
Lorraine M. Robb, Michael J. Hickey, Mark D. Wright, and Denise E. Jackson
Blood
Volume 108(6):1911-1918
September 15, 2006
©2006 by American Society of Hematology
TSSC6 is expressed on the surface and in an intracellular pool of platelets in complex with
integrin αIIbβ3.
Matt W. Goschnick et al. Blood 2006;108:1911-1918
©2006 by American Society of Hematology
TSSC6–/– mice display features of unstable hemostasis in vivo.
Matt W. Goschnick et al. Blood 2006;108:1911-1918
©2006 by American Society of Hematology
Absence of platelet TSSC6 results in secondary instability in platelet thrombi formed in vivo.
Matt W. Goschnick et al. Blood 2006;108:1911-1918
©2006 by American Society of Hematology
Delayed kinetics of clot retraction for TSSC6–/– platelets in the presence of normal integrin
αIIbβ3 expression.
Matt W. Goschnick et al. Blood 2006;108:1911-1918
©2006 by American Society of Hematology
TSSC6–/– platelets display restricted cytoskeletal reorganization upon spreading on immobilized
fibrinogen.
Matt W. Goschnick et al. Blood 2006;108:1911-1918
©2006 by American Society of Hematology
TSSC6–/– platelets display normal platelet aggregation responses to PAR-4, ADP, type 1
collagen, and calcium ionophore.
Matt W. Goschnick et al. Blood 2006;108:1911-1918
©2006 by American Society of Hematology
TSSC6–/– platelets display normal soluble FITC-fibrinogen binding, JON/A mAb binding, and
alpha granule release following treatment with thrombin, PAR-4 peptide, ADP, and ADP in
synergy with epinephrine.
Matt W. Goschnick et al. Blood 2006;108:1911-1918
©2006 by American Society of Hematology