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Proangiogenic properties of human myeloma cells:
production of angiopoietin-1 and its potential relationship
to myeloma-induced angiogenesis
by Nicola Giuliani, Simona Colla, Mirca Lazzaretti, Roberto Sala, Giovanni Roti,
Cristina Mancini, Sabrina Bonomini, Paolo Lunghi, Magda Hojden, Giovenzio
Genestreti, Mirija Svaldi, Paolo Coser, Pier Paolo Fattori, Gabriella Sammarelli, Gian
Carlo Gazzola, Regis Bataille, Camillo Almici, Cecilia Caramatti, Lina Mangoni, and
Vittorio Rizzoli
Blood
Volume 102(2):638-645
July 15, 2003
©2003 by American Society of Hematology
mRNA expression of proangiogenic factors by myeloma cells.
Nicola Giuliani et al. Blood 2003;102:638-645
©2003 by American Society of Hematology
Angiopoietin system expression by HMCLs. Ang-1, Ang-2, Tie2, and β2-microglobulin mRNA
expression by RPMI 8226, OPM-2, U266, XG-1, XG-6, and the EBV+ cell line ARH-77 were
evaluated by RT-PCR.
Nicola Giuliani et al. Blood 2003;102:638-645
©2003 by American Society of Hematology
HMCLs up-regulate Tie2 expression by endothelial cells.
Nicola Giuliani et al. Blood 2003;102:638-645
©2003 by American Society of Hematology
Proangiogenic property of HMCLs: effect of Tie2 blocking.
Nicola Giuliani et al. Blood 2003;102:638-645
©2003 by American Society of Hematology
Angiopoietin system expression in patients with MM. Ang-1 mRNA expression was evaluated by
RT-PCR in highly purified (more than 90%) MM cells obtained from 23 patients at diagnosis or
relapse.
Nicola Giuliani et al. Blood 2003;102:638-645
©2003 by American Society of Hematology
BM angiogenesis in patients with MM. Blood vessels were detected in 3-μm sections of 4%
formalin and B5 solution-fixed, paraffin-embedded specimens obtained from patients with MM.
Angiogenesis was measured as number of vessels per 400 × field and density...
Nicola Giuliani et al. Blood 2003;102:638-645
©2003 by American Society of Hematology