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SgIGSF: a new mast-cell adhesion molecule used for
attachment to fibroblasts and transcriptionally regulated
by MITF
by Akihiko Ito, Tomoko Jippo, Tomohiko Wakayama, Eiichi Morii, Yu-ichiro Koma,
Hiroaki Onda, Hiroshi Nojima, Shoichi Iseki, and Yukihiko Kitamura
Blood
Volume 101(7):2601-2608
April 1, 2003
©2003 by American Society of Hematology
Expression of SgIGSF and intercellular adhesion molecules in CMCs derived from MITF mutant
mice.(A) Expression of SgIGSF mRNA in B6+/+, B6 mi/mi , and B6 tg/tg CMCs. RNA (10 μg of
total RNA) from CMCs of 3 genotypes was electrophoresed and hybridized with t...
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology
SgIGSF expression in CMCs derived from KIT mutant mice.Lysates were prepared from CMCs
derived from mice of the indicated genotypes and were blotted with the anti-KIT and anti-SgIGSF
antibodies.
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology
SgIGSF expression in various tissues of B6 tg/tg mice.(A) Expression of the SgIGSF protein in
testis, lung, spleen, and stomach tissues of B6+/+ and B6 tg/tg mice.
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology
Immunolocalization of SgIGSF in CMCs.Immunocytochemical analysis of B6+/+ (A) and B6 tg/tg
(B) CMCs. Cytospin preparations of CMCs were fixed with methanol, reacted with the antiSgIGSF antibody, and stained with FITC-labeled secondary antibody.
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology
Expression of the SgIGSF protein in B6 tg/tg and B6 mi/mi CMCs transfected with SgIGSF
cDNA.B6 tg/tg CMCs were infected with the empty retroviral vector (tg/tg CMCVector) or the
vector containing either SgIGSF cDNA (tg/tgCMCSgIGSF) or +-MITF cDNA (tg/tgCMCM...
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology
Phenotypes of B6 tg/tg CMCs transfected with SgIGSF or +-MITF.(A) Phase contrast image of B6
tg/tg CMCs transfected with SgIGSF cDNA.
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology
Growth kinetics of SgIGSF-transfected CMCs.CMCs (2.5 × 105) were suspended in 2 mL fresh
medium containing PWM-SCM and plated onto 35-mm culture dishes.
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology
Luciferase assay and EGMSA.(A) Transactivation of the SgIGSF promoter by +-MITF.
Akihiko Ito et al. Blood 2003;101:2601-2608
©2003 by American Society of Hematology