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IL-10 and TGF-β induce alloreactive CD4+CD25– T cells to acquire regulatory cell function by Zong-ming Chen, Matthew J. O'Shaughnessy, Irene Gramaglia, Angela Panoskaltsis-Mortari, William J. Murphy, Satwant Narula, Maria G. Roncarolo, and Bruce R. Blazar Blood Volume 101(12):5076-5083 June 15, 2003 ©2003 by American Society of Hematology In vitro inhibition of naive alloresponses by the addition of IL-10/TGF-β –tolerized cells. Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology Inhibition of cell division of naive CD4+ T cells cocultured with tolerized CD4+ cells. Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology In vivo inhibition of GVHD mortality by the co-infusion of tolerized cells. Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology IL-10/TGF-β–tolerized CD4+ T cells modestly down-regulate nonspecific responses. Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology CD4+CD25+ cells are not required for the induction of tolerance to alloantigen via 8-day MLR culture with IL-10 and TGF-β (A) Primary MLR consisted of 0.5 × 106/mL B6 CD4+CD25– T-cell responders (≥ 99.6% pure) and 0.5 × 106/mL bm12 splenic stimulators c... Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology CD4+CD25– cells acquire regulatory function that inhibits naive alloresponses via 8-day MLR culture with IL-10 and TGF-β CD4+CD25+ T cells are not required for acquisition of in vitro (A) or in vivo (B) inhibitory function. Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology IL-10–/–CD4+ T cells become tolerant and develop in vitro regulatory function during MLR culture with IL-10 and TGF-β (A) IL-10–/–CD4+ cells become tolerant after MLR culture with IL-10 and TGF-β. Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology IL-10 production by IL-10/TGF-β–tolerized cells is not required for in vivo inhibition of GVHD mortality. Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology Suppression of naive alloresponses by IL-10/TGF-β–treated cells is not dependent on IL-10 and TGF-β MLR culture consisted of 105 naive B6 CD4+ T-cell responders per well and 105 bm12 splenic stimulators per well plated with and without B6 CD4+ T cells (... Zong-ming Chen et al. Blood 2003;101:5076-5083 ©2003 by American Society of Hematology
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