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From: Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method Nucleic Acids Res. 2013;41(19):e179. doi:10.1093/nar/gkt699 Nucleic Acids Res | Published by Oxford University Press 2013. This work is written by US Government employees and is in the public domain in the US. From: Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method Nucleic Acids Res. 2013;41(19):e179. doi:10.1093/nar/gkt699 Nucleic Acids Res | Published by Oxford University Press 2013. This work is written by US Government employees and is in the public domain in the US. From: Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method Nucleic Acids Res. 2013;41(19):e179. doi:10.1093/nar/gkt699 Nucleic Acids Res | Published by Oxford University Press 2013. This work is written by US Government employees and is in the public domain in the US. From: Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method Nucleic Acids Res. 2013;41(19):e179. doi:10.1093/nar/gkt699 Nucleic Acids Res | Published by Oxford University Press 2013. This work is written by US Government employees and is in the public domain in the US.