Download Powerpoint - Blood Journal

Mammalian target of rapamycin regulates neutrophil
extracellular trap formation via induction of hypoxiainducible factor 1 α
by Alison M. McInturff, Mark J. Cody, Elizabeth A. Elliott, Jared W. Glenn, Jesse W.
Rowley, Matthew T. Rondina, and Christian C. Yost
Blood
Volume 120(15):3118-3125
October 11, 2012
©2012 by American Society of Hematology
Rapamycin inhibits NET formation by human PMNs. (A) NET formation was assessed by live cell
imaging with confocal microscopy (magnification, ×20).
Alison M. McInturff et al. Blood 2012;120:3118-3125
©2012 by American Society of Hematology
HIF-1α protein accumulation is posttranscriptionally regulated by mTOR in LPS-stimulated
human PMNs. (A) We assessed HIF-1α (top) and IL-8 (bottom) transcript expression with the use
of RNA-seq in control and stimulated human PMNs isolated from a single hea...
Alison M. McInturff et al. Blood 2012;120:3118-3125
©2012 by American Society of Hematology
Pharmacologic inhibition of HIF-1α activity decreases NET formation by LPS-stimulated human
PMNs. (A) HIF-1α protein expression and NET formation were simultaneously assessed with
immunocytochemistry and confocal microscopy (magnification, ×60) in LPS-stimu...
Alison M. McInturff et al. Blood 2012;120:3118-3125
©2012 by American Society of Hematology
Genetic inhibition of HIF-1α activity decreases NET formation by LPS-stimulated surrogate
PMNs. (A) Differentiated HL-60 surrogate PMNs were transduced with a lentivirus designed to
coexpress GFP (as a marker for transduction) and an shRNA targeting HIF-1α ...
Alison M. McInturff et al. Blood 2012;120:3118-3125
©2012 by American Society of Hematology
Pharmacologic and genetic inhibition of mTOR and HIF-1α signaling inhibit NET-mediated
extracellular bacterial killing.
Alison M. McInturff et al. Blood 2012;120:3118-3125
©2012 by American Society of Hematology